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Article: Molecular dissection of the pathogen-inducible 3-deoxyanthocyanidin biosynthesis pathway in sorghum

TitleMolecular dissection of the pathogen-inducible 3-deoxyanthocyanidin biosynthesis pathway in sorghum
Authors
Keywords3-Deoxyanthocyanidins
Anthocyanin
Dihydroflavonol 4-reductase
Jasmonic acid
Phytoalexin
Sorghum
Issue Date2010
PublisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/
Citation
Plant And Cell Physiology, 2010, v. 51 n. 7, p. 1173-1185 How to Cite?
Abstract3-Deoxyanthocyanidins are the unique phytoalexins synthesized by sorghum in response to fungal inoculation. They are structurally related to anthocyanins but the final steps of their pathogen-inducible biosynthesis are not fully understood. We have identified new flavonoid structural genes from the recently completed sorghum BTx623 genome sequence. The biochemical functions of the different expressed sorghum genes were established in planta by complementation in the appropriate Arabidopsis transparent testa mutants. There is a family of nine chalcone synthase genes which are all inducible by fungal inoculation in sorghum seedlings. Specific dihydroflavonol 4-reductase (DFR) genes responsive to conditions which stimulated anthocyanin accumulation (SbDFR1) or 3-deoxyanthocyanidin production (SbDFR3) were identified. Recombinant SbDFR1 and SbDFR3 were found to function as typical DFRs by accepting dihydroflavonol substrates. On the other hand, both DFRs showed substantially lower but detectable NADPH-dependent activities toward flavanones. Reduction of flavanones to flavan-4-ols is a reaction step required for 3-deoxyanthocyanidin production. Flavanone 3-hydroxylase (F3H) converts flavanones to dihydroflavonols for anthocyanin biosynthesis. In sorghum seedlings, expression of two F3H genes was either absent or strongly suppressed during the accumulation of 3-deoxyanthocyanidins. Under such conditions, most flavanones are expected to be reduced by the pathogen-induced SbDFR3 for the formation of flavan-4-ols. Our work also revealed that 3-deoxyanthocyanidin accumulation and SbDFR3 expression were induced by methyl jasmonate treatment in sorghum roots but the stimulation effects were antagonized by salicylic acid. © 2010 The Author.
Persistent Identifierhttp://hdl.handle.net/10722/134901
ISSN
2015 Impact Factor: 4.319
2015 SCImago Journal Rankings: 2.301
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaHKU7527/06M
HKU 3/07C
HKU200911159094
Funding Information:

This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China [grant Nos. HKU7527/06M and HKU 3/07C]; the HKU Seed Funding Program [200911159094].

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorDu, Yen_HK
dc.contributor.authorChu, Hen_HK
dc.contributor.authorShih, CHen_HK
dc.contributor.authorWong, YWen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorTao, Yen_HK
dc.contributor.authorLo, Cen_HK
dc.date.accessioned2011-07-25T04:41:52Z-
dc.date.available2011-07-25T04:41:52Z-
dc.date.issued2010en_HK
dc.identifier.citationPlant And Cell Physiology, 2010, v. 51 n. 7, p. 1173-1185en_HK
dc.identifier.issn0032-0781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134901-
dc.description.abstract3-Deoxyanthocyanidins are the unique phytoalexins synthesized by sorghum in response to fungal inoculation. They are structurally related to anthocyanins but the final steps of their pathogen-inducible biosynthesis are not fully understood. We have identified new flavonoid structural genes from the recently completed sorghum BTx623 genome sequence. The biochemical functions of the different expressed sorghum genes were established in planta by complementation in the appropriate Arabidopsis transparent testa mutants. There is a family of nine chalcone synthase genes which are all inducible by fungal inoculation in sorghum seedlings. Specific dihydroflavonol 4-reductase (DFR) genes responsive to conditions which stimulated anthocyanin accumulation (SbDFR1) or 3-deoxyanthocyanidin production (SbDFR3) were identified. Recombinant SbDFR1 and SbDFR3 were found to function as typical DFRs by accepting dihydroflavonol substrates. On the other hand, both DFRs showed substantially lower but detectable NADPH-dependent activities toward flavanones. Reduction of flavanones to flavan-4-ols is a reaction step required for 3-deoxyanthocyanidin production. Flavanone 3-hydroxylase (F3H) converts flavanones to dihydroflavonols for anthocyanin biosynthesis. In sorghum seedlings, expression of two F3H genes was either absent or strongly suppressed during the accumulation of 3-deoxyanthocyanidins. Under such conditions, most flavanones are expected to be reduced by the pathogen-induced SbDFR3 for the formation of flavan-4-ols. Our work also revealed that 3-deoxyanthocyanidin accumulation and SbDFR3 expression were induced by methyl jasmonate treatment in sorghum roots but the stimulation effects were antagonized by salicylic acid. © 2010 The Author.en_HK
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/en_HK
dc.relation.ispartofPlant and Cell Physiologyen_HK
dc.subject3-Deoxyanthocyanidinsen_HK
dc.subjectAnthocyaninen_HK
dc.subjectDihydroflavonol 4-reductaseen_HK
dc.subjectJasmonic aciden_HK
dc.subjectPhytoalexinen_HK
dc.subjectSorghumen_HK
dc.subject.meshAlcohol Oxidoreductases - genetics - metabolism-
dc.subject.meshAnthocyanins - biosynthesis-
dc.subject.meshMixed Function Oxygenases - genetics - metabolism-
dc.subject.meshSesquiterpenes - metabolism-
dc.subject.meshSorghum - enzymology - genetics - microbiology-
dc.titleMolecular dissection of the pathogen-inducible 3-deoxyanthocyanidin biosynthesis pathway in sorghumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0781&volume=51&issue=7&spage=1173&epage=1185&date=2010&atitle=Molecular+dissection+of+the+pathogen-inducible+3-deoxyanthocyanidin+biosynthesis+pathway+in+sorghum-
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailChu, IK: ivankchu@hku.hken_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/pcp/pcq080en_HK
dc.identifier.pmid20529887-
dc.identifier.scopuseid_2-s2.0-77955011990en_HK
dc.identifier.hkuros186357-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955011990&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1173en_HK
dc.identifier.epage1185en_HK
dc.identifier.eissn1471-9053-
dc.identifier.isiWOS:000280318500010-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectAging: epigenetic regulation, cell cycle checkpoint control and stem cell potency-
dc.identifier.scopusauthoridLiu, H=36170607300en_HK
dc.identifier.scopusauthoridDu, Y=35725386400en_HK
dc.identifier.scopusauthoridChu, H=36870371300en_HK
dc.identifier.scopusauthoridShih, CH=55233136000en_HK
dc.identifier.scopusauthoridWong, YW=36605448800en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridTao, Y=35786636800en_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK
dc.identifier.citeulike7721651-

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