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Article: Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells

TitleLong-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells
Authors
Issue Date2011
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2011, v. 52 n. 3, p. 1539-1547 How to Cite?
AbstractPURPOSE. To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter. METHODS. A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degeneration, identify the morphologic predictors for cell survival, and estimate the rate of dendritic shrinkage. RESULTS. RGC damage was observed prospectively to begin with progressive dendritic shrinkage, followed by loss of the axon and the cell body. In a small proportion of RGCs, progressive axonal changes including fragmentation, beading, retraction, and bulb formation were also observed. RGCs with a larger dendritic field and a longer total dendritic branch length in general have a better survival probability. The rate of dendritic shrinkage was variable with a slower rate observed in cells having a larger dendritic field, a longer total dendritic branch length, and a greater distance from the optic disc. CONCLUSIONS. Estimating the probability of RGC survival and measuring the rate of dendritic shrinkage could become a new paradigm for investigating neuronal degeneration and evaluating the response of neuroprotective treatment. © 2011 The Association for Research in Vision and Ophthalmology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/134849
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
Funding AgencyGrant Number
CUHK
Glaucoma Foundation
Funding Information:

Supported by a CUHK research grant and the Glaucoma Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorLeung, CKSen_HK
dc.contributor.authorWeinreb, RNen_HK
dc.contributor.authorLi, ZWen_HK
dc.contributor.authorLiu, Sen_HK
dc.contributor.authorLindsey, JDen_HK
dc.contributor.authorChoi, Nen_HK
dc.contributor.authorLiu, Len_HK
dc.contributor.authorCheung, CYLen_HK
dc.contributor.authorYe, Cen_HK
dc.contributor.authorQiu, Ken_HK
dc.contributor.authorChen, LJen_HK
dc.contributor.authorYung, WHen_HK
dc.contributor.authorCrowston, JGen_HK
dc.contributor.authorPu, Men_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorPang, CPen_HK
dc.contributor.authorLam, DSCen_HK
dc.date.accessioned2011-07-21T06:28:24Z-
dc.date.available2011-07-21T06:28:24Z-
dc.date.issued2011en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2011, v. 52 n. 3, p. 1539-1547en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134849-
dc.description.abstractPURPOSE. To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter. METHODS. A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degeneration, identify the morphologic predictors for cell survival, and estimate the rate of dendritic shrinkage. RESULTS. RGC damage was observed prospectively to begin with progressive dendritic shrinkage, followed by loss of the axon and the cell body. In a small proportion of RGCs, progressive axonal changes including fragmentation, beading, retraction, and bulb formation were also observed. RGCs with a larger dendritic field and a longer total dendritic branch length in general have a better survival probability. The rate of dendritic shrinkage was variable with a slower rate observed in cells having a larger dendritic field, a longer total dendritic branch length, and a greater distance from the optic disc. CONCLUSIONS. Estimating the probability of RGC survival and measuring the rate of dendritic shrinkage could become a new paradigm for investigating neuronal degeneration and evaluating the response of neuroprotective treatment. © 2011 The Association for Research in Vision and Ophthalmology, Inc.en_HK
dc.languageeng-
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAxons - pathology-
dc.subject.meshDendrites - pathology-
dc.subject.meshNerve Degeneration - diagnosis-
dc.subject.meshOptic Nerve Injuries - diagnosis-
dc.subject.meshRetinal Ganglion Cells - pathology-
dc.titleLong-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-0404&volume=52&issue=3&spage=1539&epage=1547&date=2011&atitle=Long-term+in+vivo+imaging+and+measurement+of+dendritic+shrinkage+of+retinal+ganglion+cells-
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1167/iovs.10-6012en_HK
dc.identifier.pmid21245394-
dc.identifier.scopuseid_2-s2.0-79955933489en_HK
dc.identifier.hkuros186266-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955933489&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1539en_HK
dc.identifier.epage1547en_HK
dc.identifier.isiWOS:000288965300041-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, CKS=8834590400en_HK
dc.identifier.scopusauthoridWeinreb, RN=35380128600en_HK
dc.identifier.scopusauthoridLi, ZW=39061857200en_HK
dc.identifier.scopusauthoridLiu, S=35277464600en_HK
dc.identifier.scopusauthoridLindsey, JD=7201528916en_HK
dc.identifier.scopusauthoridChoi, N=37030738600en_HK
dc.identifier.scopusauthoridLiu, L=36170705900en_HK
dc.identifier.scopusauthoridCheung, CYL=35276903300en_HK
dc.identifier.scopusauthoridYe, C=36106620900en_HK
dc.identifier.scopusauthoridQiu, K=34877307500en_HK
dc.identifier.scopusauthoridChen, LJ=35321846100en_HK
dc.identifier.scopusauthoridYung, WH=7103137893en_HK
dc.identifier.scopusauthoridCrowston, JG=26643353200en_HK
dc.identifier.scopusauthoridPu, M=16073321400en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridPang, CP=24423687500en_HK
dc.identifier.scopusauthoridLam, DSC=35500200200en_HK

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