File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Development and characterization of genetic mapping resources for the turkey (Meleagris gallopavo)

TitleDevelopment and characterization of genetic mapping resources for the turkey (Meleagris gallopavo)
Authors
Issue Date1999
PublisherOxford University Press. The Journal's web site is located at http://jhered.oxfordjournals.org/
Citation
Journal Of Heredity, 1999, v. 90 n. 1, p. 240-242 How to Cite?
AbstractThe development and partial characterization of turkey genomic libraries enriched for TG, GAT, and CCT simple sequence repeats (SSR) are described. The primary library, established using conventional methods, was enriched for each of the three SSR by single-primer polymerase chain reaction (PCR). The three enriched libraries were screened by standard hybridization and washing protocols under moderate to high stringency conditions. The utility of a fraction of the markers was evaluated based on the polymorphism of PCR- amplified products in a backcross reference DNA panel. The panel consisted of genomic DNA samples from three back-crossed families developed from a cross of a wild male turkey to three inbred Orlopp line C females. A total of 181 sequences from positive clones have been characterized and deposited in GenBank. About 60% of the 60 primer pairs designed from SSR-containing sequences detected polymorphism in the reference DNA panel. The turkey genomic DNA reference panel, the enriched libraries, and the markers described here provide an opportunity to begin to characterize the turkey genome and to develop a useful public genetic map for this economically important species.
Persistent Identifierhttp://hdl.handle.net/10722/134755
ISSN
2015 Impact Factor: 2.075
2015 SCImago Journal Rankings: 1.024
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, HBen_HK
dc.contributor.authorSong, YQen_HK
dc.contributor.authorHsei, Men_HK
dc.contributor.authorZahorchak, Ren_HK
dc.contributor.authorChiu, Jen_HK
dc.contributor.authorTeuscher, Cen_HK
dc.contributor.authorSmith, EJen_HK
dc.date.accessioned2011-07-14T07:02:47Z-
dc.date.available2011-07-14T07:02:47Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Heredity, 1999, v. 90 n. 1, p. 240-242en_HK
dc.identifier.issn0022-1503en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134755-
dc.description.abstractThe development and partial characterization of turkey genomic libraries enriched for TG, GAT, and CCT simple sequence repeats (SSR) are described. The primary library, established using conventional methods, was enriched for each of the three SSR by single-primer polymerase chain reaction (PCR). The three enriched libraries were screened by standard hybridization and washing protocols under moderate to high stringency conditions. The utility of a fraction of the markers was evaluated based on the polymorphism of PCR- amplified products in a backcross reference DNA panel. The panel consisted of genomic DNA samples from three back-crossed families developed from a cross of a wild male turkey to three inbred Orlopp line C females. A total of 181 sequences from positive clones have been characterized and deposited in GenBank. About 60% of the 60 primer pairs designed from SSR-containing sequences detected polymorphism in the reference DNA panel. The turkey genomic DNA reference panel, the enriched libraries, and the markers described here provide an opportunity to begin to characterize the turkey genome and to develop a useful public genetic map for this economically important species.en_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jhered.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Heredityen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosome Mapping/*veterinaryen_US
dc.subject.meshDNA Probesen_US
dc.subject.meshFemaleen_US
dc.subject.mesh*Genomic Libraryen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reaction/veterinaryen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshTurkeys/*geneticsen_US
dc.titleDevelopment and characterization of genetic mapping resources for the turkey (Meleagris gallopavo)en_HK
dc.typeArticleen_HK
dc.identifier.emailSong, YQ:songy@hkucc.hku.hken_HK
dc.identifier.authoritySong, YQ=rp00488en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jhered/90.1.240en_HK
dc.identifier.pmid9987934-
dc.identifier.scopuseid_2-s2.0-0032928288en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032928288&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue1en_HK
dc.identifier.spage240en_HK
dc.identifier.epage242en_HK
dc.identifier.isiWOS:000078225400046-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHuang, HB=20134378500en_HK
dc.identifier.scopusauthoridSong, YQ=7404921212en_HK
dc.identifier.scopusauthoridHsei, M=6508050759en_HK
dc.identifier.scopusauthoridZahorchak, R=6602900265en_HK
dc.identifier.scopusauthoridChiu, J=55137328500en_HK
dc.identifier.scopusauthoridTeuscher, C=7005902859en_HK
dc.identifier.scopusauthoridSmith, EJ=7408614063en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats