Article: Screening for PS1 mutations in a referral-based series of AD cases: 21 Novel mutations
| Title | Screening for PS1 mutations in a referral-based series of AD cases: 21 Novel mutations |
|---|---|
| Authors | Rogaeva, EA3 Fafel, KC4 Song, YQ3 Medeiros, H3 Sato, C3 Liang, Y3 Richard, E3 Rogaev, EI2 3 Frommelt, P5 Sadovnick, AD6 Meschino, W7 Rockwood, K8 Boss, MA4 Mayeux, R1 St GeorgeHyslop, P3 |
| Issue Date | 2001 |
| Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.neurology.org |
| Citation | Neurology, 2001, v. 57 n. 4, p. 621-625 [How to Cite?] |
| Abstract | Background: Mutations in the presenilin-1 gene (PS1) account for a majority of patients with early onset familial AD. However, the clinical indications and algorithms for genetic testing in dementia are still evolving. Methods: The entire open reading frame of the PS1 gene was sequenced in a series of 414 consecutive patients referred for diagnostic testing, including 372 patients with AD and 42 asymptomatic persons with a strong family history of AD. Results: Forty-eight independent patients screened had a PS1 mutation including 21 novel mutations. In addition, 3% of subjects (11/413) had a known polymorphism, the Glu318Gly substitution. The majority of the mutations were missense substitutions but there were three insertions and Δexon 10 mutation. With six exceptions (codons 35, 178, 352, 354, 358, and 365) most of the mutations occurred at residues conserved in the homologous PS2 gene or in PS1 of other species. Conclusions: Eleven percent of a referral-based series of patients with AD can be explained by coding sequence mutations in the PS1 gene. The high frequency of PS1 mutations in this study indicates that screening for PS1 mutations in AD is likely to be successful, especially when directed at patients with a positive family history with onset before 60 years (90% of those with PS1 mutations were affected by age 60 years). This will also have significance for the secondary identification of at-risk relatives who might be candidates for future prophylactic therapies for AD. |
| ISSN | 0028-3878 2011 Impact Factor: 8.312 2011 SCImago Journal Rankings: 0.534 |
| ISI Accession Number ID | WOS:000170623900009 |
| References | References in Scopus |
| dc.contributor.author | Rogaeva, EA |
|---|---|
| dc.contributor.author | Fafel, KC |
| dc.contributor.author | Song, YQ |
| dc.contributor.author | Medeiros, H |
| dc.contributor.author | Sato, C |
| dc.contributor.author | Liang, Y |
| dc.contributor.author | Richard, E |
| dc.contributor.author | Rogaev, EI |
| dc.contributor.author | Frommelt, P |
| dc.contributor.author | Sadovnick, AD |
| dc.contributor.author | Meschino, W |
| dc.contributor.author | Rockwood, K |
| dc.contributor.author | Boss, MA |
| dc.contributor.author | Mayeux, R |
| dc.contributor.author | St GeorgeHyslop, P |
| dc.date.accessioned | 2011-07-14T07:02:29Z |
| dc.date.available | 2011-07-14T07:02:29Z |
| dc.date.issued | 2001 |
| dc.description.abstract | Background: Mutations in the presenilin-1 gene (PS1) account for a majority of patients with early onset familial AD. However, the clinical indications and algorithms for genetic testing in dementia are still evolving. Methods: The entire open reading frame of the PS1 gene was sequenced in a series of 414 consecutive patients referred for diagnostic testing, including 372 patients with AD and 42 asymptomatic persons with a strong family history of AD. Results: Forty-eight independent patients screened had a PS1 mutation including 21 novel mutations. In addition, 3% of subjects (11/413) had a known polymorphism, the Glu318Gly substitution. The majority of the mutations were missense substitutions but there were three insertions and Δexon 10 mutation. With six exceptions (codons 35, 178, 352, 354, 358, and 365) most of the mutations occurred at residues conserved in the homologous PS2 gene or in PS1 of other species. Conclusions: Eleven percent of a referral-based series of patients with AD can be explained by coding sequence mutations in the PS1 gene. The high frequency of PS1 mutations in this study indicates that screening for PS1 mutations in AD is likely to be successful, especially when directed at patients with a positive family history with onset before 60 years (90% of those with PS1 mutations were affected by age 60 years). This will also have significance for the secondary identification of at-risk relatives who might be candidates for future prophylactic therapies for AD. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Neurology, 2001, v. 57 n. 4, p. 621-625 [How to Cite?] |
| dc.identifier.epage | 625 |
| dc.identifier.isi | WOS:000170623900009 |
| dc.identifier.issn | 0028-3878 2011 Impact Factor: 8.312 2011 SCImago Journal Rankings: 0.534 |
| dc.identifier.issue | 4 |
| dc.identifier.pmid | 11524469 |
| dc.identifier.scopus | eid_2-s2.0-0035964209 |
| dc.identifier.spage | 621 |
| dc.identifier.uri | http://hdl.handle.net/10722/134745 |
| dc.identifier.volume | 57 |
| dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.neurology.org |
| dc.publisher.place | United States |
| dc.relation.ispartof | Neurology |
| dc.relation.references | References in Scopus |
| dc.subject.mesh | Adult |
| dc.subject.mesh | Aged |
| dc.subject.mesh | Aged, 80 and over |
| dc.subject.mesh | Alzheimer Disease/diagnosis/*genetics |
| dc.subject.mesh | Genetic Testing/*methods |
| dc.subject.mesh | Humans |
| dc.subject.mesh | Membrane Proteins/*genetics |
| dc.subject.mesh | Middle Aged |
| dc.subject.mesh | Mutation/*genetics |
| dc.subject.mesh | Presenilin-1 |
| dc.subject.mesh | Referral and Consultation |
| dc.subject.mesh | Survival Analysis |
| dc.title | Screening for PS1 mutations in a referral-based series of AD cases: 21 Novel mutations |
| dc.type | Article |
Author Affiliations
- null
- Russian Academy of Medical Sciences
- University Health Network
- Athena Diagnostics, Inc.
- Asklepios Klinik Schaufling
- The University of British Columbia
- North York General Hospital
- Dalhousie University

