File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of the chicken galanin type I receptor (GalR1) and a novel GalR1-like receptor (GalR1-L)

TitleCharacterization of the chicken galanin type I receptor (GalR1) and a novel GalR1-like receptor (GalR1-L)
Authors
KeywordsChicken
G protein-coupled receptor
Galanin
Galanin type I receptor
Galanin type I-like receptor
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2011, v. 170 n. 2, p. 391-400 How to Cite?
AbstractGalanin is a multi-functional neuropeptide that is widely distributed in the mammalian central nervous system and peripheral tissues. It exerts multiple physiological functions through interaction with 3 known G protein-coupled receptors (GPCR), namely, galanin type I, II and III (GalR1, 2 and 3) receptors, which have only been identified in mammals. In this study, we reported the cloning and characterization of chicken galanin type I receptor (GalR1) and a novel galanin receptor with considerable homology to chicken GalR1, which herein is designated as galanin type I-like receptor (GalR1-L). Chicken GalR1 and GalR1-L full-length cDNAs were cloned from chicken brain and small intestine tissue, respectively. The former encodes a protein of 357 amino acids that shares 84-86% amino acid sequence identities with its mammalian counterparts, whereas the latter encodes a 363-amino acid protein with comparatively lower identities (55-56%) to the mammalian GalR1. Using reverse transcription (RT)-PCR assays, we examined the expression of both receptors in adult chicken tissues. Both receptors were found to be widely distributed in the tissues examined, including brain, small intestine, kidney, ovary, pancreas, pituitary and spleen. Interestingly, cGalR1 expression was detected in different regions of chicken oviduct, while cGalR1-L expression was restricted to the vagina. Using a pGL3-CRE luciferase reporter system, chicken galanin peptide (1-29) was demonstrated to inhibit both basal and forskolin-stimulated luciferase activities, in dose-dependent manners, through the cAMP-mediated signaling pathway in Chinese hamster ovary (CHO) cells expressing either cGalR1 or cGalR1-L, thus suggesting the functional couplings of both receptors to G i proteins. Together, the characterization of chicken GalR1 and GalR1-L provides a better understanding of the physiological roles of galanin in avian species. © 2010.
Persistent Identifierhttp://hdl.handle.net/10722/134726
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of the Hong Kong GovernmentHKU7345/03M
National Natural Science Foundation of China30771171
30871338
National High Technology Research and Development Program of China2007AA10Z163
Funding Information:

This work was supported by Grants from the Research Grant Council of the Hong Kong Government (HKU7345/03M), the National Natural Science Foundation of China (30771171, 30871338), and the National High Technology Research and Development Program of China (2007AA10Z163).

References

 

DC FieldValueLanguage
dc.contributor.authorHo, JCWen_HK
dc.contributor.authorKwok, AHYen_HK
dc.contributor.authorZhao, Den_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2011-07-12T08:11:32Z-
dc.date.available2011-07-12T08:11:32Z-
dc.date.issued2011en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 2011, v. 170 n. 2, p. 391-400en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134726-
dc.description.abstractGalanin is a multi-functional neuropeptide that is widely distributed in the mammalian central nervous system and peripheral tissues. It exerts multiple physiological functions through interaction with 3 known G protein-coupled receptors (GPCR), namely, galanin type I, II and III (GalR1, 2 and 3) receptors, which have only been identified in mammals. In this study, we reported the cloning and characterization of chicken galanin type I receptor (GalR1) and a novel galanin receptor with considerable homology to chicken GalR1, which herein is designated as galanin type I-like receptor (GalR1-L). Chicken GalR1 and GalR1-L full-length cDNAs were cloned from chicken brain and small intestine tissue, respectively. The former encodes a protein of 357 amino acids that shares 84-86% amino acid sequence identities with its mammalian counterparts, whereas the latter encodes a 363-amino acid protein with comparatively lower identities (55-56%) to the mammalian GalR1. Using reverse transcription (RT)-PCR assays, we examined the expression of both receptors in adult chicken tissues. Both receptors were found to be widely distributed in the tissues examined, including brain, small intestine, kidney, ovary, pancreas, pituitary and spleen. Interestingly, cGalR1 expression was detected in different regions of chicken oviduct, while cGalR1-L expression was restricted to the vagina. Using a pGL3-CRE luciferase reporter system, chicken galanin peptide (1-29) was demonstrated to inhibit both basal and forskolin-stimulated luciferase activities, in dose-dependent manners, through the cAMP-mediated signaling pathway in Chinese hamster ovary (CHO) cells expressing either cGalR1 or cGalR1-L, thus suggesting the functional couplings of both receptors to G i proteins. Together, the characterization of chicken GalR1 and GalR1-L provides a better understanding of the physiological roles of galanin in avian species. © 2010.en_HK
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectChickenen_HK
dc.subjectG protein-coupled receptoren_HK
dc.subjectGalaninen_HK
dc.subjectGalanin type I receptoren_HK
dc.subjectGalanin type I-like receptoren_HK
dc.titleCharacterization of the chicken galanin type I receptor (GalR1) and a novel GalR1-like receptor (GalR1-L)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-6480&volume=170&issue=2&spage=391&epage=400&date=2011&atitle=Characterization+of+the+chicken+galanin+type+I+receptor+(GalR1)+and+a+novel+GalR1-like+receptor+(GalR1-L)-
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygcen.2010.10.011en_HK
dc.identifier.pmid20977910-
dc.identifier.scopuseid_2-s2.0-78650678777en_HK
dc.identifier.hkuros186073-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650678777&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume170en_HK
dc.identifier.issue2en_HK
dc.identifier.spage391en_HK
dc.identifier.epage400en_HK
dc.identifier.isiWOS:000286367600019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, JCW=37761650300en_HK
dc.identifier.scopusauthoridKwok, AHY=27168105100en_HK
dc.identifier.scopusauthoridZhao, D=55475680100en_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.citeulike8135074-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats