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Article: ROCK inhibition facilitates the generation of human-induced pluripotent stem cells in a defined, feeder-, and serum-free system

TitleROCK inhibition facilitates the generation of human-induced pluripotent stem cells in a defined, feeder-, and serum-free system
Authors
KeywordsSpecies Index: Animalia
Mus
Issue Date2010
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/clo
Citation
Cellular Reprogramming, 2010, v. 12 n. 6, p. 641-653 How to Cite?
AbstractHuman-induced pluripotent stem cells (iPSCs) generated from human adult somatic cells through reprogramming hold great promises for future regenerative medicine. However, exposure of human iPSCs to animal feeder and serum in the process of their generation and maintenance imposes risk of transmitting animal pathogens to human subjects, thus hindering the potential therapeutic applications. Here, we report the successful generation of human iPSCs in a feeder-independent culture system with defined factors. Two stable human iPSC lines were established from primary human dermal fibroblasts of two healthy volunteers. These human iPSCs expressed a panel of pluripotency markers including stage-specific embryonic antigen (SSEA)-4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, and alkaline phosphatase, while maintaining normal karyotypes and the exogenous reprogramming factors being silenced. In addition, these human iPSCs can differentiate along lineages representative of the three embryonic germ layers upon formation of embryoid bodies, indicating their pluripotency. Furthermore, subcutaneous transplantation of these cells into immunodeficient mice resulted in teratoma formation in 6 to 8 weeks. Our findings are an important step toward generating patient-specific iPSCs in a more clinically compliant manner by eliminating the need of animal feeder cells and animal serum. © 2010 Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/134669
ISSN
2015 Impact Factor: 1.462
2015 SCImago Journal Rankings: 0.681
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU8CRF09
HKU 780110M
Funding Information:

This work was supported by the Hong Kong Research Grant Council (HKU8CRF09 to Prof. Tse and Dr. Siu, and HKU 780110M to Prof. Tse).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLai, WHen_HK
dc.contributor.authorHo, JCYen_HK
dc.contributor.authorLee, YKen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorAu, KWen_HK
dc.contributor.authorChan, YCen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorSiu, CWen_HK
dc.date.accessioned2011-07-05T08:23:58Z-
dc.date.available2011-07-05T08:23:58Z-
dc.date.issued2010en_HK
dc.identifier.citationCellular Reprogramming, 2010, v. 12 n. 6, p. 641-653en_HK
dc.identifier.issn2152-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134669-
dc.description.abstractHuman-induced pluripotent stem cells (iPSCs) generated from human adult somatic cells through reprogramming hold great promises for future regenerative medicine. However, exposure of human iPSCs to animal feeder and serum in the process of their generation and maintenance imposes risk of transmitting animal pathogens to human subjects, thus hindering the potential therapeutic applications. Here, we report the successful generation of human iPSCs in a feeder-independent culture system with defined factors. Two stable human iPSC lines were established from primary human dermal fibroblasts of two healthy volunteers. These human iPSCs expressed a panel of pluripotency markers including stage-specific embryonic antigen (SSEA)-4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, and alkaline phosphatase, while maintaining normal karyotypes and the exogenous reprogramming factors being silenced. In addition, these human iPSCs can differentiate along lineages representative of the three embryonic germ layers upon formation of embryoid bodies, indicating their pluripotency. Furthermore, subcutaneous transplantation of these cells into immunodeficient mice resulted in teratoma formation in 6 to 8 weeks. Our findings are an important step toward generating patient-specific iPSCs in a more clinically compliant manner by eliminating the need of animal feeder cells and animal serum. © 2010 Mary Ann Liebert, Inc.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/cloen_HK
dc.relation.ispartofCellular Reprogrammingen_HK
dc.subjectSpecies Index: Animaliaen_US
dc.subjectMusen_US
dc.titleROCK inhibition facilitates the generation of human-induced pluripotent stem cells in a defined, feeder-, and serum-free systemen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, KM: skykmng@hkucc.hku.hken_HK
dc.identifier.emailChan, YC: ycchan09@hku.hken_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hken_HK
dc.identifier.authorityNg, KM=rp01670en_HK
dc.identifier.authorityChan, YC=rp01502en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1089/cell.2010.0051en_HK
dc.identifier.pmid20858051-
dc.identifier.pmcidPMC2993021-
dc.identifier.scopuseid_2-s2.0-78649507841en_HK
dc.identifier.hkuros169709-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649507841&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue6en_HK
dc.identifier.spage641en_HK
dc.identifier.epage653en_HK
dc.identifier.eissn2152-4998-
dc.identifier.isiWOS:000284619400002-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectAutologous Induced Pluripotent Stem Cells Derived Cardiomyocytes for Cardiac Repair in Porcine Ischemic Cardiomyopathy-
dc.identifier.scopusauthoridLai, WH=18434390500en_HK
dc.identifier.scopusauthoridHo, JCY=7402650173en_HK
dc.identifier.scopusauthoridLee, YK=25958641200en_HK
dc.identifier.scopusauthoridNg, KM=25122990200en_HK
dc.identifier.scopusauthoridAu, KW=9738204200en_HK
dc.identifier.scopusauthoridChan, YC=7403676116en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK

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