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Article: Generation of induced pluripotent stem cells from urine
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TitleGeneration of induced pluripotent stem cells from urine
 
AuthorsZhou, T4
Benda, C2 4
Duzinger, S2
Huang, Y4
Li, X4
Li, Y4
Guo, X4
Cao, G4
Chen, S4
Hao, L4
Chan, YC3
Ng, KM3
Ho, JC3
Wieser, M2 5
Wu, J4
Redl, H6
Tse, HF3
Grillari, J2 1
GrillariVoglauer, R2 1
Pei, D4
Esteban, MA4
 
Issue Date2011
 
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
 
CitationJournal Of The American Society Of Nephrology, 2011, v. 22 n. 7, p. 1221-1228 [How to Cite?]
DOI: http://dx.doi.org/10.1681/ASN.2011010106
 
AbstractForced expression of selected transcription factors can transform somatic cells into embryonic stem cell (ESC)-like cells, termed induced pluripotent stem cells (iPSCs). There is no consensus regarding the preferred tissue from which to harvest donor cells for reprogramming into iPSCs, and some donor cell types may be more prone than others to accumulation of epigenetic imprints and somatic cell mutations. Here, we present a simple, reproducible, noninvasive method for generating human iPSCs from renal tubular cells present in urine. This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs. Copyright © 2011 by the American Society of Nephrology.
 
DescriptionComment in J Am Soc Nephrol. 2011 Jul;22(7):1179-1180
 
ISSN1046-6673
2013 Impact Factor: 9.466
 
DOIhttp://dx.doi.org/10.1681/ASN.2011010106
 
PubMed Central IDPMC3137570
 
ISI Accession Number IDWOS:000293203400010
Funding AgencyGrant Number
Ministry of Science and Technology of China2011CB965200
Chinese Academy of SciencesKSCX2-YW-R244
KSCX2-YW-G-075 to 18
Ministry of Science and Technology China2007CB948002
Ministry of Science and Technology International Technology Cooperation Program2010DFB30430
Bureau of Science and Technology of Guangzhou Municipality, China2010U1-E00521
Austrian Science FundS93-06
Genome Research Austria GEN-AU820982
Funding Information:

This work was supported by grants from the 973 program of Ministry of Science and Technology of China (2011CB965200) and Chinese Academy of Sciences (KSCX2-YW-R244) to M.A.E.; by grants from the 973 Program of Ministry of Science and Technology China (2007CB948002), Ministry of Science and Technology International Technology Cooperation Program (2010DFB30430), Knowledge Innovation Project of The Chinese Academy of Sciences KSCX2-YW-G-075 to 18, and Bureau of Science and Technology of Guangzhou Municipality, China (2010U1-E00521) to D.P.; and by grants by the Austrian Science Fund (S93-06) and Genome Research Austria GEN-AU (820982) to J.G. and R.G.-V. We thank all members of our laboratories for helpful suggestions. J.G. and R.G.-V. are recent cofounders and directors of Evercyte.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhou, T
 
dc.contributor.authorBenda, C
 
dc.contributor.authorDuzinger, S
 
dc.contributor.authorHuang, Y
 
dc.contributor.authorLi, X
 
dc.contributor.authorLi, Y
 
dc.contributor.authorGuo, X
 
dc.contributor.authorCao, G
 
dc.contributor.authorChen, S
 
dc.contributor.authorHao, L
 
dc.contributor.authorChan, YC
 
dc.contributor.authorNg, KM
 
dc.contributor.authorHo, JC
 
dc.contributor.authorWieser, M
 
dc.contributor.authorWu, J
 
dc.contributor.authorRedl, H
 
dc.contributor.authorTse, HF
 
dc.contributor.authorGrillari, J
 
dc.contributor.authorGrillariVoglauer, R
 
dc.contributor.authorPei, D
 
dc.contributor.authorEsteban, MA
 
dc.date.accessioned2011-06-17T09:20:40Z
 
dc.date.available2011-06-17T09:20:40Z
 
dc.date.issued2011
 
dc.description.abstractForced expression of selected transcription factors can transform somatic cells into embryonic stem cell (ESC)-like cells, termed induced pluripotent stem cells (iPSCs). There is no consensus regarding the preferred tissue from which to harvest donor cells for reprogramming into iPSCs, and some donor cell types may be more prone than others to accumulation of epigenetic imprints and somatic cell mutations. Here, we present a simple, reproducible, noninvasive method for generating human iPSCs from renal tubular cells present in urine. This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs. Copyright © 2011 by the American Society of Nephrology.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionComment in J Am Soc Nephrol. 2011 Jul;22(7):1179-1180
 
dc.identifier.citationJournal Of The American Society Of Nephrology, 2011, v. 22 n. 7, p. 1221-1228 [How to Cite?]
DOI: http://dx.doi.org/10.1681/ASN.2011010106
 
dc.identifier.citeulike10689762
 
dc.identifier.doihttp://dx.doi.org/10.1681/ASN.2011010106
 
dc.identifier.epage1228
 
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dc.identifier.hkuros185836
 
dc.identifier.isiWOS:000293203400010
Funding AgencyGrant Number
Ministry of Science and Technology of China2011CB965200
Chinese Academy of SciencesKSCX2-YW-R244
KSCX2-YW-G-075 to 18
Ministry of Science and Technology China2007CB948002
Ministry of Science and Technology International Technology Cooperation Program2010DFB30430
Bureau of Science and Technology of Guangzhou Municipality, China2010U1-E00521
Austrian Science FundS93-06
Genome Research Austria GEN-AU820982
Funding Information:

This work was supported by grants from the 973 program of Ministry of Science and Technology of China (2011CB965200) and Chinese Academy of Sciences (KSCX2-YW-R244) to M.A.E.; by grants from the 973 Program of Ministry of Science and Technology China (2007CB948002), Ministry of Science and Technology International Technology Cooperation Program (2010DFB30430), Knowledge Innovation Project of The Chinese Academy of Sciences KSCX2-YW-G-075 to 18, and Bureau of Science and Technology of Guangzhou Municipality, China (2010U1-E00521) to D.P.; and by grants by the Austrian Science Fund (S93-06) and Genome Research Austria GEN-AU (820982) to J.G. and R.G.-V. We thank all members of our laboratories for helpful suggestions. J.G. and R.G.-V. are recent cofounders and directors of Evercyte.

 
dc.identifier.issn1046-6673
2013 Impact Factor: 9.466
 
dc.identifier.issue7
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC3137570
 
dc.identifier.pmid21636641
 
dc.identifier.scopuseid_2-s2.0-79960122427
 
dc.identifier.spage1221
 
dc.identifier.urihttp://hdl.handle.net/10722/134440
 
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dc.languageeng
 
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of the American Society of Nephrology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshGene Transfer Techniques
 
dc.subject.meshInduced Pluripotent Stem Cells
 
dc.subject.meshKidney Tubules - cytology
 
dc.subject.meshUrine - cytology
 
dc.titleGeneration of induced pluripotent stem cells from urine
 
dc.typeArticle
 
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Author Affiliations
  1. Evercyte GmbH
  2. Universitat fur Bodenkultur Wien
  3. The University of Hong Kong
  4. Chinese Academy of Sciences
  5. Austrian Centre of Industrial Biotechnology (ACIB GmbH)
  6. null