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Article: Transcriptional profiling of osseointegration in humans

TitleTranscriptional profiling of osseointegration in humans
Authors
KeywordsGene expression
Human
Hydrophilic surface
Oral implants
Osseointegration
Transcriptome
Issue Date2011
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
Citation
Clinical Oral Implants Research, 2011, v. 22 n. 4, p. 373-381 How to Cite?
Abstract
Objective: To determine the temporal gene expression profile associated with the early healing events during osseointegration in a human model. Material and methods: Nine solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with a chemically modified surface (SLActive) were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 4, 7 and 14. Results: Gene ontology (GO) analysis of the temporal transcriptional changes was characteristic of a maturing, osteogenic process over the course of the study (4-14 days). At day 4, a gene expression profile associated with proliferation and immuno-inflammatory processes was predominant. However, by day 14, by far the most predominant mechanisms were associated with skeletogenesis, with the GO categories of skeletal system development, bone development and ossification being predominant, with the majority of changes occurring between days 7 and 14. Furthermore, the biological processes of angiogenesis and neurogenesis were also predominant by day 14. In terms of signal transduction, I-κB kinase/NF-κB cascade was predominant at day 4, whereas TGF-β/BMP, Wnt and Notch signalling were all associated with the osteogenic process over the duration of the study. Furthermore, Ras and Rho protein signal transduction was regulated throughout the osseointegration process. Conclusion: The temporal transcriptional changes during osseointegration involve the expression of proliferation and immuno-inflammatory response associated genes during the early stages of osseointegration, which are ultimately replaced by genes associated with the biological processes of skeletogenesis, angiogenesis and neurogenesis. The early immuno-inflammatory changes appear to be regulated via the I-κB kinase/NF-κB cascade, whereas the later osteogenesis-related mechanisms are regulated by TGF-β/BMP, Notch and Wnt signaling. © 2011 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/134339
ISSN
2013 Impact Factor: 3.123
ISI Accession Number ID
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study has been supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

References

 

DC FieldValueLanguage
dc.contributor.authorIvanovski, Sen_HK
dc.contributor.authorHamlet, Sen_HK
dc.contributor.authorSalvi, GEen_HK
dc.contributor.authorHuynhBa, Gen_HK
dc.contributor.authorBosshardt, DDen_HK
dc.contributor.authorLang, NPen_HK
dc.contributor.authorDonos, Nen_HK
dc.date.accessioned2011-06-17T09:18:03Z-
dc.date.available2011-06-17T09:18:03Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 373-381en_HK
dc.identifier.issn0905-7161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134339-
dc.description.abstractObjective: To determine the temporal gene expression profile associated with the early healing events during osseointegration in a human model. Material and methods: Nine solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with a chemically modified surface (SLActive) were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 4, 7 and 14. Results: Gene ontology (GO) analysis of the temporal transcriptional changes was characteristic of a maturing, osteogenic process over the course of the study (4-14 days). At day 4, a gene expression profile associated with proliferation and immuno-inflammatory processes was predominant. However, by day 14, by far the most predominant mechanisms were associated with skeletogenesis, with the GO categories of skeletal system development, bone development and ossification being predominant, with the majority of changes occurring between days 7 and 14. Furthermore, the biological processes of angiogenesis and neurogenesis were also predominant by day 14. In terms of signal transduction, I-κB kinase/NF-κB cascade was predominant at day 4, whereas TGF-β/BMP, Wnt and Notch signalling were all associated with the osteogenic process over the duration of the study. Furthermore, Ras and Rho protein signal transduction was regulated throughout the osseointegration process. Conclusion: The temporal transcriptional changes during osseointegration involve the expression of proliferation and immuno-inflammatory response associated genes during the early stages of osseointegration, which are ultimately replaced by genes associated with the biological processes of skeletogenesis, angiogenesis and neurogenesis. The early immuno-inflammatory changes appear to be regulated via the I-κB kinase/NF-κB cascade, whereas the later osteogenesis-related mechanisms are regulated by TGF-β/BMP, Notch and Wnt signaling. © 2011 John Wiley & Sons A/S.en_HK
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLRen_HK
dc.relation.ispartofClinical Oral Implants Researchen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectGene expressionen_HK
dc.subjectHumanen_HK
dc.subjectHydrophilic surfaceen_HK
dc.subjectOral implantsen_HK
dc.subjectOsseointegrationen_HK
dc.subjectTranscriptomeen_HK
dc.titleTranscriptional profiling of osseointegration in humansen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0905-7161&volume=22&issue=4&spage=373&epage=381&date=2011&atitle=Transcriptional+profiling+of+osseointegration+in+humans-
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_HK
dc.identifier.authorityLang, NP=rp00031en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0501.2010.02112.xen_HK
dc.identifier.pmid21561479en_HK
dc.identifier.scopuseid_2-s2.0-79952508777en_HK
dc.identifier.hkuros185685en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952508777&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue4en_HK
dc.identifier.spage373en_HK
dc.identifier.epage381en_HK
dc.identifier.isiWOS:000288214300004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridIvanovski, S=6601979085en_HK
dc.identifier.scopusauthoridHamlet, S=7006308569en_HK
dc.identifier.scopusauthoridSalvi, GE=35600695300en_HK
dc.identifier.scopusauthoridHuynhBa, G=25825004200en_HK
dc.identifier.scopusauthoridBosshardt, DD=6603806230en_HK
dc.identifier.scopusauthoridLang, NP=7201577367en_HK
dc.identifier.scopusauthoridDonos, N=7004314492en_HK
dc.identifier.citeulike8993411-

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