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Article: Transcriptional profiling of osseointegration in humans
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TitleTranscriptional profiling of osseointegration in humans
 
AuthorsIvanovski, S3
Hamlet, S3
Salvi, GE4
HuynhBa, G4
Bosshardt, DD4
Lang, NP2
Donos, N1
 
KeywordsGene expression
Human
Hydrophilic surface
Oral implants
Osseointegration
Transcriptome
 
Issue Date2011
 
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
 
CitationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 373-381 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0501.2010.02112.x
 
AbstractObjective: To determine the temporal gene expression profile associated with the early healing events during osseointegration in a human model. Material and methods: Nine solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with a chemically modified surface (SLActive) were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 4, 7 and 14. Results: Gene ontology (GO) analysis of the temporal transcriptional changes was characteristic of a maturing, osteogenic process over the course of the study (4-14 days). At day 4, a gene expression profile associated with proliferation and immuno-inflammatory processes was predominant. However, by day 14, by far the most predominant mechanisms were associated with skeletogenesis, with the GO categories of skeletal system development, bone development and ossification being predominant, with the majority of changes occurring between days 7 and 14. Furthermore, the biological processes of angiogenesis and neurogenesis were also predominant by day 14. In terms of signal transduction, I-κB kinase/NF-κB cascade was predominant at day 4, whereas TGF-β/BMP, Wnt and Notch signalling were all associated with the osteogenic process over the duration of the study. Furthermore, Ras and Rho protein signal transduction was regulated throughout the osseointegration process. Conclusion: The temporal transcriptional changes during osseointegration involve the expression of proliferation and immuno-inflammatory response associated genes during the early stages of osseointegration, which are ultimately replaced by genes associated with the biological processes of skeletogenesis, angiogenesis and neurogenesis. The early immuno-inflammatory changes appear to be regulated via the I-κB kinase/NF-κB cascade, whereas the later osteogenesis-related mechanisms are regulated by TGF-β/BMP, Notch and Wnt signaling. © 2011 John Wiley & Sons A/S.
 
ISSN0905-7161
2013 Impact Factor: 3.123
 
DOIhttp://dx.doi.org/10.1111/j.1600-0501.2010.02112.x
 
ISI Accession Number IDWOS:000288214300004
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study has been supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorIvanovski, S
 
dc.contributor.authorHamlet, S
 
dc.contributor.authorSalvi, GE
 
dc.contributor.authorHuynhBa, G
 
dc.contributor.authorBosshardt, DD
 
dc.contributor.authorLang, NP
 
dc.contributor.authorDonos, N
 
dc.date.accessioned2011-06-17T09:18:03Z
 
dc.date.available2011-06-17T09:18:03Z
 
dc.date.issued2011
 
dc.description.abstractObjective: To determine the temporal gene expression profile associated with the early healing events during osseointegration in a human model. Material and methods: Nine solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with a chemically modified surface (SLActive) were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 4, 7 and 14. Results: Gene ontology (GO) analysis of the temporal transcriptional changes was characteristic of a maturing, osteogenic process over the course of the study (4-14 days). At day 4, a gene expression profile associated with proliferation and immuno-inflammatory processes was predominant. However, by day 14, by far the most predominant mechanisms were associated with skeletogenesis, with the GO categories of skeletal system development, bone development and ossification being predominant, with the majority of changes occurring between days 7 and 14. Furthermore, the biological processes of angiogenesis and neurogenesis were also predominant by day 14. In terms of signal transduction, I-κB kinase/NF-κB cascade was predominant at day 4, whereas TGF-β/BMP, Wnt and Notch signalling were all associated with the osteogenic process over the duration of the study. Furthermore, Ras and Rho protein signal transduction was regulated throughout the osseointegration process. Conclusion: The temporal transcriptional changes during osseointegration involve the expression of proliferation and immuno-inflammatory response associated genes during the early stages of osseointegration, which are ultimately replaced by genes associated with the biological processes of skeletogenesis, angiogenesis and neurogenesis. The early immuno-inflammatory changes appear to be regulated via the I-κB kinase/NF-κB cascade, whereas the later osteogenesis-related mechanisms are regulated by TGF-β/BMP, Notch and Wnt signaling. © 2011 John Wiley & Sons A/S.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 373-381 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0501.2010.02112.x
 
dc.identifier.citeulike8993411
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0501.2010.02112.x
 
dc.identifier.epage381
 
dc.identifier.hkuros185685
 
dc.identifier.isiWOS:000288214300004
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study has been supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

 
dc.identifier.issn0905-7161
2013 Impact Factor: 3.123
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid21561479
 
dc.identifier.scopuseid_2-s2.0-79952508777
 
dc.identifier.spage373
 
dc.identifier.urihttp://hdl.handle.net/10722/134339
 
dc.identifier.volume22
 
dc.languageeng
 
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical Oral Implants Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe definitive version is available at www3.interscience.wiley.com
 
dc.subjectGene expression
 
dc.subjectHuman
 
dc.subjectHydrophilic surface
 
dc.subjectOral implants
 
dc.subjectOsseointegration
 
dc.subjectTranscriptome
 
dc.titleTranscriptional profiling of osseointegration in humans
 
dc.typeArticle
 
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Author Affiliations
  1. UCL Eastman Dental Institute
  2. The University of Hong Kong
  3. Griffith University
  4. Universität Bern