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Article: Gene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface

TitleGene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
Authors
KeywordsGene expression
Human
Hydrophilic surface
Oral implants
Osseointegration
Surface roughness
Transcriptome
Issue Date2011
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
Citation
Clinical Oral Implants Research, 2011, v. 22 n. 4, p. 365-372 How to Cite?
AbstractObjectives: To compare the gene expression profile of osseointegration associated with a moderately rough and a chemically modified hydrophilic moderately rough surface in a human model. Material and methods: Eighteen solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with either a moderately rough (SLA) or a chemically modified moderately rough (SLActive) surface were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between the SLA and SLActive surfaces at days 4, 7 and 14. Results: There were no functionally relevant gene ontology categories that were over-represented in the list of genes that were differentially expressed at day 4. However, by day 7, osteogenesis- and angiogenesis-associated gene expression were up-regulated on the SLActive surface. Osteogenesis and angiogenesis appeared to be regulated by BMP and VEGF signalling, respectively. By day 14, VEGF signalling remains up-regulated on the SLActive surface, while BMP signalling was up-regulated on the SLA surface in what appeared to be a delayed compensatory response. Furthermore, neurogenesis was a prominent biological process within the list of differentially expressed genes, and it was influenced by both surfaces. Conclusions: Compared with SLA, SLActive exerts a pro-osteogenic and pro-angiogenic influence on gene expression at day 7 following implant insertion, which may be responsible for the superior osseointegrative properties of this surface. © 2011 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/134338
ISSN
2014 Impact Factor: 3.889
2014 SCImago Journal Rankings: 1.290
ISI Accession Number ID
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study was supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

References

 

DC FieldValueLanguage
dc.contributor.authorDonos, Nen_HK
dc.contributor.authorHamlet, Sen_HK
dc.contributor.authorLang, NPen_HK
dc.contributor.authorSalvi, GEen_HK
dc.contributor.authorHuynhBa, Gen_HK
dc.contributor.authorBosshardt, DDen_HK
dc.contributor.authorIvanovski, Sen_HK
dc.date.accessioned2011-06-17T09:18:03Z-
dc.date.available2011-06-17T09:18:03Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 365-372en_HK
dc.identifier.issn0905-7161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134338-
dc.description.abstractObjectives: To compare the gene expression profile of osseointegration associated with a moderately rough and a chemically modified hydrophilic moderately rough surface in a human model. Material and methods: Eighteen solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with either a moderately rough (SLA) or a chemically modified moderately rough (SLActive) surface were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between the SLA and SLActive surfaces at days 4, 7 and 14. Results: There were no functionally relevant gene ontology categories that were over-represented in the list of genes that were differentially expressed at day 4. However, by day 7, osteogenesis- and angiogenesis-associated gene expression were up-regulated on the SLActive surface. Osteogenesis and angiogenesis appeared to be regulated by BMP and VEGF signalling, respectively. By day 14, VEGF signalling remains up-regulated on the SLActive surface, while BMP signalling was up-regulated on the SLA surface in what appeared to be a delayed compensatory response. Furthermore, neurogenesis was a prominent biological process within the list of differentially expressed genes, and it was influenced by both surfaces. Conclusions: Compared with SLA, SLActive exerts a pro-osteogenic and pro-angiogenic influence on gene expression at day 7 following implant insertion, which may be responsible for the superior osseointegrative properties of this surface. © 2011 John Wiley & Sons A/S.en_HK
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLRen_HK
dc.relation.ispartofClinical Oral Implants Researchen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectGene expressionen_HK
dc.subjectHumanen_HK
dc.subjectHydrophilic surfaceen_HK
dc.subjectOral implantsen_HK
dc.subjectOsseointegrationen_HK
dc.subjectSurface roughnessen_HK
dc.subjectTranscriptomeen_HK
dc.titleGene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surfaceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0905-7161&volume=22&issue=4&spage=365&epage=372&date=2011&atitle=Gene+expression+profile+of+osseointegration+of+a+hydrophilic+compared+with+a+hydrophobic+microrough+implant+surface-
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_HK
dc.identifier.authorityLang, NP=rp00031en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0501.2010.02113.xen_HK
dc.identifier.pmid21561478-
dc.identifier.scopuseid_2-s2.0-79952506850en_HK
dc.identifier.hkuros185682en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952506850&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue4en_HK
dc.identifier.spage365en_HK
dc.identifier.epage372en_HK
dc.identifier.isiWOS:000288214300003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDonos, N=7004314492en_HK
dc.identifier.scopusauthoridHamlet, S=7006308569en_HK
dc.identifier.scopusauthoridLang, NP=7201577367en_HK
dc.identifier.scopusauthoridSalvi, GE=35600695300en_HK
dc.identifier.scopusauthoridHuynhBa, G=25825004200en_HK
dc.identifier.scopusauthoridBosshardt, DD=6603806230en_HK
dc.identifier.scopusauthoridIvanovski, S=6601979085en_HK
dc.identifier.citeulike8993412-

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