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Article: Gene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
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TitleGene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
 
AuthorsDonos, N1
Hamlet, S3
Lang, NP2
Salvi, GE4
HuynhBa, G4
Bosshardt, DD4
Ivanovski, S3
 
KeywordsGene expression
Human
Hydrophilic surface
Oral implants
Osseointegration
Surface roughness
Transcriptome
 
Issue Date2011
 
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
 
CitationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 365-372 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0501.2010.02113.x
 
AbstractObjectives: To compare the gene expression profile of osseointegration associated with a moderately rough and a chemically modified hydrophilic moderately rough surface in a human model. Material and methods: Eighteen solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with either a moderately rough (SLA) or a chemically modified moderately rough (SLActive) surface were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between the SLA and SLActive surfaces at days 4, 7 and 14. Results: There were no functionally relevant gene ontology categories that were over-represented in the list of genes that were differentially expressed at day 4. However, by day 7, osteogenesis- and angiogenesis-associated gene expression were up-regulated on the SLActive surface. Osteogenesis and angiogenesis appeared to be regulated by BMP and VEGF signalling, respectively. By day 14, VEGF signalling remains up-regulated on the SLActive surface, while BMP signalling was up-regulated on the SLA surface in what appeared to be a delayed compensatory response. Furthermore, neurogenesis was a prominent biological process within the list of differentially expressed genes, and it was influenced by both surfaces. Conclusions: Compared with SLA, SLActive exerts a pro-osteogenic and pro-angiogenic influence on gene expression at day 7 following implant insertion, which may be responsible for the superior osseointegrative properties of this surface. © 2011 John Wiley & Sons A/S.
 
ISSN0905-7161
2012 Impact Factor: 3.433
2012 SCImago Journal Rankings: 1.154
 
DOIhttp://dx.doi.org/10.1111/j.1600-0501.2010.02113.x
 
ISI Accession Number IDWOS:000288214300003
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study was supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDonos, N
 
dc.contributor.authorHamlet, S
 
dc.contributor.authorLang, NP
 
dc.contributor.authorSalvi, GE
 
dc.contributor.authorHuynhBa, G
 
dc.contributor.authorBosshardt, DD
 
dc.contributor.authorIvanovski, S
 
dc.date.accessioned2011-06-17T09:18:03Z
 
dc.date.available2011-06-17T09:18:03Z
 
dc.date.issued2011
 
dc.description.abstractObjectives: To compare the gene expression profile of osseointegration associated with a moderately rough and a chemically modified hydrophilic moderately rough surface in a human model. Material and methods: Eighteen solid screw-type cylindrical titanium implants, 4mm long and 2.8mm wide, with either a moderately rough (SLA) or a chemically modified moderately rough (SLActive) surface were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between the SLA and SLActive surfaces at days 4, 7 and 14. Results: There were no functionally relevant gene ontology categories that were over-represented in the list of genes that were differentially expressed at day 4. However, by day 7, osteogenesis- and angiogenesis-associated gene expression were up-regulated on the SLActive surface. Osteogenesis and angiogenesis appeared to be regulated by BMP and VEGF signalling, respectively. By day 14, VEGF signalling remains up-regulated on the SLActive surface, while BMP signalling was up-regulated on the SLA surface in what appeared to be a delayed compensatory response. Furthermore, neurogenesis was a prominent biological process within the list of differentially expressed genes, and it was influenced by both surfaces. Conclusions: Compared with SLA, SLActive exerts a pro-osteogenic and pro-angiogenic influence on gene expression at day 7 following implant insertion, which may be responsible for the superior osseointegrative properties of this surface. © 2011 John Wiley & Sons A/S.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical Oral Implants Research, 2011, v. 22 n. 4, p. 365-372 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0501.2010.02113.x
 
dc.identifier.citeulike8993412
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0501.2010.02113.x
 
dc.identifier.epage372
 
dc.identifier.hkuros185682
 
dc.identifier.isiWOS:000288214300003
Funding AgencyGrant Number
ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland)371/04
Funding Information:

This study was supported by a grant from the ITI Foundation for the Promotion of Implantology (ITI Basel, Switzerland) Nr. 371/04.

 
dc.identifier.issn0905-7161
2012 Impact Factor: 3.433
2012 SCImago Journal Rankings: 1.154
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid21561478
 
dc.identifier.scopuseid_2-s2.0-79952506850
 
dc.identifier.spage365
 
dc.identifier.urihttp://hdl.handle.net/10722/134338
 
dc.identifier.volume22
 
dc.languageeng
 
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical Oral Implants Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe definitive version is available at www3.interscience.wiley.com
 
dc.subjectGene expression
 
dc.subjectHuman
 
dc.subjectHydrophilic surface
 
dc.subjectOral implants
 
dc.subjectOsseointegration
 
dc.subjectSurface roughness
 
dc.subjectTranscriptome
 
dc.titleGene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
 
dc.typeArticle
 
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Author Affiliations
  1. UCL Eastman Dental Institute
  2. The University of Hong Kong
  3. Griffith University
  4. Universität Bern