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Article: Determination of A 3,4-diaminopyridine in plasma by liquid chromatography with electrochemical detection using solid-phase extraction

TitleDetermination of A 3,4-diaminopyridine in plasma by liquid chromatography with electrochemical detection using solid-phase extraction
Authors
Keywords3,4-Diaminopyridine
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb
Citation
Journal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2004, v. 805 n. 2, p. 261-266 How to Cite?
AbstractIn order to quantify a small amount of a drug, 3,4-diaminopyridine (3,4-DAP), in animal plasma samples, an analytical method was developed. It involved an extraction of 3,4-DAP and phenylephrine, used as internal standard (IS), from plasma with solid-phase extraction (SPE) on C18 cartridges. This analytical method is a hyphenated technique based on high-performance liquid chromatography with electrochemical detection (HPLC-EC) whose purpose is to obtain first a sensitive method and second a satisfying separation between 3,4-DAP and phenylephrine. The analytical method is accurate, specific, and linear between 10 and 500 μg of 3,4-DAP per litre. The recovery of 3,4-DAP is estimated at 70.8% with a 95% confidence interval of (66.0-75.6%). Intermediate precision was evaluated on three quality control samples; the intra-day precision was estimated at 13.5, 9.1, 7.8% and the inter-day precision at 17.9, 8.4, 9.3%. The limit of quantification of the method was evaluated at 10 μg l-1. First toxicokinetic parameters determined on dogs plasma samples after one 3,4-DAP oral administration of 1 mg kg-1 were: C max=395.7 μg l-1; Tmax=15 min; t 1/2=113.6 min; Clearance/F=16.8 ml kg-1 min-1 and Vd/F=2.7 l kg-1. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/134254
ISSN
2015 Impact Factor: 2.687
2015 SCImago Journal Rankings: 0.947
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGoulayDufaÿ, Sen_HK
dc.contributor.authorDo, Ben_HK
dc.contributor.authorLe Hoang, MDen_HK
dc.contributor.authorRaust, JAen_HK
dc.contributor.authorGraffard, Hen_HK
dc.contributor.authorGuyon, Fen_HK
dc.contributor.authorPradeau, Den_HK
dc.date.accessioned2011-06-14T09:16:09Z-
dc.date.available2011-06-14T09:16:09Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2004, v. 805 n. 2, p. 261-266en_HK
dc.identifier.issn1570-0232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134254-
dc.description.abstractIn order to quantify a small amount of a drug, 3,4-diaminopyridine (3,4-DAP), in animal plasma samples, an analytical method was developed. It involved an extraction of 3,4-DAP and phenylephrine, used as internal standard (IS), from plasma with solid-phase extraction (SPE) on C18 cartridges. This analytical method is a hyphenated technique based on high-performance liquid chromatography with electrochemical detection (HPLC-EC) whose purpose is to obtain first a sensitive method and second a satisfying separation between 3,4-DAP and phenylephrine. The analytical method is accurate, specific, and linear between 10 and 500 μg of 3,4-DAP per litre. The recovery of 3,4-DAP is estimated at 70.8% with a 95% confidence interval of (66.0-75.6%). Intermediate precision was evaluated on three quality control samples; the intra-day precision was estimated at 13.5, 9.1, 7.8% and the inter-day precision at 17.9, 8.4, 9.3%. The limit of quantification of the method was evaluated at 10 μg l-1. First toxicokinetic parameters determined on dogs plasma samples after one 3,4-DAP oral administration of 1 mg kg-1 were: C max=395.7 μg l-1; Tmax=15 min; t 1/2=113.6 min; Clearance/F=16.8 ml kg-1 min-1 and Vd/F=2.7 l kg-1. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromben_HK
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_HK
dc.subject3,4-Diaminopyridineen_HK
dc.titleDetermination of A 3,4-diaminopyridine in plasma by liquid chromatography with electrochemical detection using solid-phase extractionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=15700232&volume=805&issue=2&spage=261&epage=266&date=2004&atitle=Determination+of+A+3,4-diaminopyridine+in+plasma+by+liquid+chromatography+with+electrochemical+detection+using+solid-phase+extraction-
dc.identifier.emailGoulayDufaÿ, S: sdufay@hku.hken_HK
dc.identifier.authorityGoulayDufaÿ, S=rp01491en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jchromb.2004.03.010en_HK
dc.identifier.pmid15135099-
dc.identifier.scopuseid_2-s2.0-2342513462en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2342513462&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume805en_HK
dc.identifier.issue2en_HK
dc.identifier.spage261en_HK
dc.identifier.epage266en_HK
dc.identifier.isiWOS:000221573700011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridGoulayDufaÿ, S=6507768346en_HK
dc.identifier.scopusauthoridDo, B=7003399176en_HK
dc.identifier.scopusauthoridLe Hoang, MD=6603079461en_HK
dc.identifier.scopusauthoridRaust, JA=6603461316en_HK
dc.identifier.scopusauthoridGraffard, H=6507325901en_HK
dc.identifier.scopusauthoridGuyon, F=7006952930en_HK
dc.identifier.scopusauthoridPradeau, D=7003279930en_HK

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