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Article: Sensitive quantification of diphemanil methyl sulphate in human plasma by liquid chromatography-tandem mass spectrometry

TitleSensitive quantification of diphemanil methyl sulphate in human plasma by liquid chromatography-tandem mass spectrometry
Authors
KeywordsDiphemanil methylsulphate
HPLC
Plasma
Quaternary ammonium
Tandem mass spectrometry
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb
Citation
Journal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2007, v. 845 n. 1, p. 104-108 How to Cite?
AbstractA simple detection system with a high-performance liquid chromatography (HPLC) with positive ionisation-tandem mass spectrometry (ESI-MS/MS) for determining diphemanil methylsulphate (DMS) levels in human plasma using 4-diphemanylmethylene,1-methylpiperidine as an internal standard (I.S.), is proposed. The acquisition was performed with the multiple reactional monitoring (MRM) mode, by monitoring the transitions: m/z 278 > 262 for DMS and m/z 263 > 247 for the I.S. The method involved a simple single-step deproteinisation with acetonitrile. The analyte was chromatographed on a Zorbax® C18 reversed-phase chromatographic column by isocratic elution with 10-3 M ammonium acetate and 10-3 M hexafluorobutyric acid, adjusted to pH 7.0 with ammoniac/acetonitrile (40/60, v/v). The results were linear over the studied range (0.5-50.0 ng mL-1) and the total analysis time for each run was 10 min. The mean extraction apparent recoveries expressed at the 95% intervals of confidence were 94-104% for DMS and 92-106% for the I.S. The intra- and inter-assay precisions were 4.6-8.4% and 2.9-10.6%, respectively. The limit of quantification was 0.15 ng mL-1. The devised assay was successfully applied to the residual concentrations monitoring in infant. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/134252
ISSN
2015 Impact Factor: 2.687
2015 SCImago Journal Rankings: 0.947
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDo, Ben_HK
dc.contributor.authorGoulayDufaÿ, Sen_HK
dc.contributor.authorLe Hoang, MDen_HK
dc.contributor.authorAdoui, Nen_HK
dc.contributor.authorGraffard, Hen_HK
dc.contributor.authorGuyon, Fen_HK
dc.contributor.authorPradeau, Den_HK
dc.date.accessioned2011-06-14T09:01:58Z-
dc.date.available2011-06-14T09:01:58Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2007, v. 845 n. 1, p. 104-108en_HK
dc.identifier.issn1570-0232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134252-
dc.description.abstractA simple detection system with a high-performance liquid chromatography (HPLC) with positive ionisation-tandem mass spectrometry (ESI-MS/MS) for determining diphemanil methylsulphate (DMS) levels in human plasma using 4-diphemanylmethylene,1-methylpiperidine as an internal standard (I.S.), is proposed. The acquisition was performed with the multiple reactional monitoring (MRM) mode, by monitoring the transitions: m/z 278 > 262 for DMS and m/z 263 > 247 for the I.S. The method involved a simple single-step deproteinisation with acetonitrile. The analyte was chromatographed on a Zorbax® C18 reversed-phase chromatographic column by isocratic elution with 10-3 M ammonium acetate and 10-3 M hexafluorobutyric acid, adjusted to pH 7.0 with ammoniac/acetonitrile (40/60, v/v). The results were linear over the studied range (0.5-50.0 ng mL-1) and the total analysis time for each run was 10 min. The mean extraction apparent recoveries expressed at the 95% intervals of confidence were 94-104% for DMS and 92-106% for the I.S. The intra- and inter-assay precisions were 4.6-8.4% and 2.9-10.6%, respectively. The limit of quantification was 0.15 ng mL-1. The devised assay was successfully applied to the residual concentrations monitoring in infant. © 2006 Elsevier B.V. All rights reserved.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromben_HK
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_HK
dc.subjectDiphemanil methylsulphateen_HK
dc.subjectHPLCen_HK
dc.subjectPlasmaen_HK
dc.subjectQuaternary ammoniumen_HK
dc.subjectTandem mass spectrometryen_HK
dc.titleSensitive quantification of diphemanil methyl sulphate in human plasma by liquid chromatography-tandem mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=15700232&volume=845&issue=1&spage=104&epage=108&date=2007&atitle=Sensitive+quantification+of+diphemanil+methyl+sulphate+in+human+plasma+by+liquid+chromatography-tandem+mass+spectrometry-
dc.identifier.emailGoulayDufaÿ, S: sdufay@hku.hken_HK
dc.identifier.authorityGoulayDufaÿ, S=rp01491en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jchromb.2006.07.057en_HK
dc.identifier.pmid16962397-
dc.identifier.scopuseid_2-s2.0-33751514570en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33751514570&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume845en_HK
dc.identifier.issue1en_HK
dc.identifier.spage104en_HK
dc.identifier.epage108en_HK
dc.identifier.isiWOS:000243704200011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridDo, B=7003399176en_HK
dc.identifier.scopusauthoridGoulayDufaÿ, S=6507768346en_HK
dc.identifier.scopusauthoridLe Hoang, MD=6603079461en_HK
dc.identifier.scopusauthoridAdoui, N=15130602300en_HK
dc.identifier.scopusauthoridGraffard, H=6507325901en_HK
dc.identifier.scopusauthoridGuyon, F=7006952930en_HK
dc.identifier.scopusauthoridPradeau, D=7003279930en_HK

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