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Article: Isolation of P1 bacteriophage clones containing large contiguous segments of the human and mouse loci for the T-cell coreceptor molecule CD8

TitleIsolation of P1 bacteriophage clones containing large contiguous segments of the human and mouse loci for the T-cell coreceptor molecule CD8
Authors
Issue Date1994
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneanabioeng
Citation
Genetic Analysis Techniques And Applications, 1994, v. 11 n. 5-6, p. 129-139 How to Cite?
AbstractThe T-lymphocyte coreceptor molecules CD8 (composed of CD8α and CD8β chains) and CD4 undergo a complex pattern of regulated expression during T- cell maturation. In the thymus, the most immature cells progress from expressing neither molecule (the double-negative [DN] stage) to an intermediate stage at which both are coexpressed (the double-positive [DP] stage). As a result of thymic selection and further differentiation, DP cells give rise to the most mature thymic cells and peripheral T cells that express either CD8 or CD4 (the single-positive [SP] stage). Our previous studies of the transcriptional regulatory mechanisms controlling CD8α expression during the DN → DP and DP → SP transitions suggest the existence of important cis- acting elements located a considerable distance from the CD8α gene and that these elements might serve to regulate both CD8α and CD8β. While both genes and intergenic DNA span ~60 kb in the mouse, the relevant cis elements could lie either within or beyond this region. As a result, we sought to isolate large contiguous segments of DNA in P1 bacteriophage that covered at least this region from the mouse and human CD8 locus. Our initial physical characterization of these clones demonstrates the value of the P1 system as all isolated clones were found to contain single contiguous 85- to 95-kb segments of DNA that are faithful replicas of the chromosomal locus. The presence of abundant native flanking DNA both upstream and downstream of the intact coding regions will make these clones extremely useful for identifying physiological CD8 cis-active regulatory elements by virtue of their ability to direct appropriate lineage- and stage-specific expression in transfected and transgenic T cells.
Persistent Identifierhttp://hdl.handle.net/10722/133888
ISSN
2001 Impact Factor: 2.94
2002 SCImago Journal Rankings: 0.830
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, XLen_HK
dc.contributor.authorKui Lai Leeen_HK
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorParnes, JRen_HK
dc.contributor.authorShepherd, NSen_HK
dc.contributor.authorChamberlain, JWen_HK
dc.date.accessioned2011-06-03T07:19:23Z-
dc.date.available2011-06-03T07:19:23Z-
dc.date.issued1994en_HK
dc.identifier.citationGenetic Analysis Techniques And Applications, 1994, v. 11 n. 5-6, p. 129-139en_HK
dc.identifier.issn1050-3862en_HK
dc.identifier.urihttp://hdl.handle.net/10722/133888-
dc.description.abstractThe T-lymphocyte coreceptor molecules CD8 (composed of CD8α and CD8β chains) and CD4 undergo a complex pattern of regulated expression during T- cell maturation. In the thymus, the most immature cells progress from expressing neither molecule (the double-negative [DN] stage) to an intermediate stage at which both are coexpressed (the double-positive [DP] stage). As a result of thymic selection and further differentiation, DP cells give rise to the most mature thymic cells and peripheral T cells that express either CD8 or CD4 (the single-positive [SP] stage). Our previous studies of the transcriptional regulatory mechanisms controlling CD8α expression during the DN → DP and DP → SP transitions suggest the existence of important cis- acting elements located a considerable distance from the CD8α gene and that these elements might serve to regulate both CD8α and CD8β. While both genes and intergenic DNA span ~60 kb in the mouse, the relevant cis elements could lie either within or beyond this region. As a result, we sought to isolate large contiguous segments of DNA in P1 bacteriophage that covered at least this region from the mouse and human CD8 locus. Our initial physical characterization of these clones demonstrates the value of the P1 system as all isolated clones were found to contain single contiguous 85- to 95-kb segments of DNA that are faithful replicas of the chromosomal locus. The presence of abundant native flanking DNA both upstream and downstream of the intact coding regions will make these clones extremely useful for identifying physiological CD8 cis-active regulatory elements by virtue of their ability to direct appropriate lineage- and stage-specific expression in transfected and transgenic T cells.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneanabioengen_HK
dc.relation.ispartofGenetic Analysis Techniques and Applicationsen_HK
dc.rightsAppropriate Bibliographic Citation:Authors posting Accepted Author Manuscript online should later add a citation for the Published Journal Article indicating that the Article was subsequently published, and may mention the journal title provided that they add the following text at the beginning of the document: “NOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#”-
dc.subject.meshAntigens, CD8 - genetics-
dc.subject.meshAntigens, Ly - genetics-
dc.subject.meshBacteriophage P1 - genetics-
dc.subject.meshChromosome Mapping-
dc.subject.meshGenetic Vectors - genetics-
dc.titleIsolation of P1 bacteriophage clones containing large contiguous segments of the human and mouse loci for the T-cell coreceptor molecule CD8en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1050-3862&volume=11&issue=5-6&spage=129&epage=139&date=1994&atitle=Isolation+of+P1+bacteriophage+clones+containing+large+contiguous+segments+of+the+human+and+mouse+loci+for+the+T-cell+coreceptor+molecule+CD8-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/1050-3862(94)90033-7en_HK
dc.identifier.pmid7710778en_HK
dc.identifier.scopuseid_2-s2.0-43949152220en_HK
dc.identifier.hkuros120994-
dc.identifier.volume11en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage129en_HK
dc.identifier.epage139en_HK
dc.identifier.isiWOS:A1994QA79400002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, XL=35546346800en_HK
dc.identifier.scopusauthoridKui Lai Lee=16170915900en_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridParnes, JR=7006506147en_HK
dc.identifier.scopusauthoridShepherd, NS=7102245384en_HK
dc.identifier.scopusauthoridChamberlain, JW=7202327071en_HK

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