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Article: Impaired ability of Cftr knockout mice to control lung infection with Pseudomonas aeruginosa

TitleImpaired ability of Cftr knockout mice to control lung infection with Pseudomonas aeruginosa
Authors
Issue Date1998
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
American Journal Of Respiratory And Critical Care Medicine, 1998, v. 157 n. 4 I, p. 1253-1262 How to Cite?
AbstractThe present study was aimed at investigating the innate susceptibility of C57BL/6-Cftr(unc)/Cftr(unc) knockout [B6-Cftr (-/-)] mice to pulmonary infection with Pseudomonas aeruginosa. Our results indicate that 58.4% of B6- Cftr (-/-) mice died within 6 d following lung infection with 105 P. aeruginosa entrapped in agar beads, whereas only 12.1% of B6-Cftr (+/+) mice died over the same period of time. Moreover, the number of bacteria recovered from the lungs of B6-Cftr (-/-) mice 3 and 6 d after infection was significantly higher than that observed in their littermate controls. No correlation was found between the weight or age of the animals and the number of viable bacteria recovered from the lungs of mice. Histopathological examination of lung sections from P. aeruginoso-infected mice revealed that the infection results in a severe bronchopneumonia. Both B6-Cftr (-/-) knockout mice and their littermate controls developed similar lung pathology during the course of infection. Overall, results reported in the present study suggest that a defect at the Cftr locus leads to an exacerbation of P. aeruginosa lung infection resulting in a dramatically increased mortality rate and higher bacterial load.
Persistent Identifierhttp://hdl.handle.net/10722/133887
ISSN
2015 Impact Factor: 13.118
2015 SCImago Journal Rankings: 5.832
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGosselin, Den_HK
dc.contributor.authorStevenson, MMen_HK
dc.contributor.authorCowley, EAen_HK
dc.contributor.authorGriesenbach, Uen_HK
dc.contributor.authorEidelman, DHen_HK
dc.contributor.authorBoule, Men_HK
dc.contributor.authorTam, MFen_HK
dc.contributor.authorKent, Gen_HK
dc.contributor.authorSkamene, Een_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRadzioch, Den_HK
dc.date.accessioned2011-06-03T06:29:37Z-
dc.date.available2011-06-03T06:29:37Z-
dc.date.issued1998en_HK
dc.identifier.citationAmerican Journal Of Respiratory And Critical Care Medicine, 1998, v. 157 n. 4 I, p. 1253-1262en_HK
dc.identifier.issn1073-449Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/133887-
dc.description.abstractThe present study was aimed at investigating the innate susceptibility of C57BL/6-Cftr(unc)/Cftr(unc) knockout [B6-Cftr (-/-)] mice to pulmonary infection with Pseudomonas aeruginosa. Our results indicate that 58.4% of B6- Cftr (-/-) mice died within 6 d following lung infection with 105 P. aeruginosa entrapped in agar beads, whereas only 12.1% of B6-Cftr (+/+) mice died over the same period of time. Moreover, the number of bacteria recovered from the lungs of B6-Cftr (-/-) mice 3 and 6 d after infection was significantly higher than that observed in their littermate controls. No correlation was found between the weight or age of the animals and the number of viable bacteria recovered from the lungs of mice. Histopathological examination of lung sections from P. aeruginoso-infected mice revealed that the infection results in a severe bronchopneumonia. Both B6-Cftr (-/-) knockout mice and their littermate controls developed similar lung pathology during the course of infection. Overall, results reported in the present study suggest that a defect at the Cftr locus leads to an exacerbation of P. aeruginosa lung infection resulting in a dramatically increased mortality rate and higher bacterial load.en_HK
dc.languageeng-
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.orgen_HK
dc.relation.ispartofAmerican Journal of Respiratory and Critical Care Medicineen_HK
dc.subject.meshBronchopneumonia - complications - microbiology - pathology-
dc.subject.meshCystic Fibrosis - complications-
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - genetics-
dc.subject.meshLung Diseases - complications - immunology - microbiology - pathology-
dc.subject.meshPseudomonas Infections - complications - immunology - microbiology - pathology-
dc.titleImpaired ability of Cftr knockout mice to control lung infection with Pseudomonas aeruginosaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1073-449X&volume=157&issue=4&spage=1253&epage=1262&date=1998&atitle=Impaired+ability+of+Cftr+knockout+mice+to+control+lung+infection+with+pseudomonas+aeruginosa-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid9563748-
dc.identifier.scopuseid_2-s2.0-0031979299en_HK
dc.identifier.hkuros121081-
dc.identifier.volume157en_HK
dc.identifier.issue4 Ien_HK
dc.identifier.spage1253en_HK
dc.identifier.epage1262en_HK
dc.identifier.isiWOS:000073024500038-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGosselin, D=7003373462en_HK
dc.identifier.scopusauthoridStevenson, MM=7201804305en_HK
dc.identifier.scopusauthoridCowley, EA=7003702485en_HK
dc.identifier.scopusauthoridGriesenbach, U=6701826068en_HK
dc.identifier.scopusauthoridEidelman, DH=7005951484en_HK
dc.identifier.scopusauthoridBoule, M=8530298000en_HK
dc.identifier.scopusauthoridTam, MF=7102267809en_HK
dc.identifier.scopusauthoridKent, G=34770664200en_HK
dc.identifier.scopusauthoridSkamene, E=7005192517en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRadzioch, D=7007012582en_HK

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