Article: Analysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage

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TitleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
AuthorsBreitman, ML1
Bryce, DM1
Giddens, E1
Clapoff, S1
Goring, D1
Tsui, LC1
Klintworth, GK1
Bernstein, A1
Issue Date1989
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
CitationDevelopment, 1989, v. 106 n. 3, p. 457-463 [How to Cite?]
AbstractTransgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.
ISSN0950-1991
2011 Impact Factor: 6.596
2011 SCImago Journal Rankings: 1.626
ISI Accession Number IDWOS:A1989AF20300005
DC Field
Value
dc.contributor.authorBreitman, ML
dc.contributor.authorBryce, DM
dc.contributor.authorGiddens, E
dc.contributor.authorClapoff, S
dc.contributor.authorGoring, D
dc.contributor.authorTsui, LC
dc.contributor.authorKlintworth, GK
dc.contributor.authorBernstein, A
dc.date.accessioned2011-06-01T08:20:15Z
dc.date.available2011-06-01T08:20:15Z
dc.date.issued1989
dc.description.abstractTransgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationDevelopment, 1989, v. 106 n. 3, p. 457-463 [How to Cite?]
dc.identifier.epage463
dc.identifier.isiWOS:A1989AF20300005
dc.identifier.issn0950-1991
2011 Impact Factor: 6.596
2011 SCImago Journal Rankings: 1.626
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid2598819
dc.identifier.scopuseid_2-s2.0-0024362876
dc.identifier.spage457
dc.identifier.urihttp://hdl.handle.net/10722/133867
dc.identifier.volume106
dc.languageeng
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
dc.publisher.placeUnited Kingdom
dc.relation.ispartofDevelopment
dc.subject.meshCrystallins - genetics
dc.subject.meshDNA - genetics
dc.subject.meshGene Expression
dc.subject.meshLens, Crystalline - abnormalities - metabolism - pathology
dc.subject.meshMicrophthalmos - genetics
dc.titleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
dc.typeArticle
Author Affiliations
  1. Mount Sinai Hospital, Toronto