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Article: Analysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage

TitleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
Authors
Issue Date1989
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
Citation
Development, 1989, v. 106 n. 3, p. 457-463 How to Cite?
Abstract
Transgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.
Persistent Identifierhttp://hdl.handle.net/10722/133867
ISSN
2013 Impact Factor: 6.273
ISI Accession Number ID

 

Author Affiliations
  1. Mount Sinai Hospital, Toronto
DC FieldValueLanguage
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorBryce, DMen_HK
dc.contributor.authorGiddens, Een_HK
dc.contributor.authorClapoff, Sen_HK
dc.contributor.authorGoring, Den_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorKlintworth, GKen_HK
dc.contributor.authorBernstein, Aen_HK
dc.date.accessioned2011-06-01T08:20:15Z-
dc.date.available2011-06-01T08:20:15Z-
dc.date.issued1989en_HK
dc.identifier.citationDevelopment, 1989, v. 106 n. 3, p. 457-463en_HK
dc.identifier.issn0950-1991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/133867-
dc.description.abstractTransgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.en_HK
dc.languageeng-
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.orgen_HK
dc.relation.ispartofDevelopmenten_HK
dc.subject.meshCrystallins - genetics-
dc.subject.meshDNA - genetics-
dc.subject.meshGene Expression-
dc.subject.meshLens, Crystalline - abnormalities - metabolism - pathology-
dc.subject.meshMicrophthalmos - genetics-
dc.titleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineageen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-1991&volume=106&issue=3&spage=457&epage=463&date=1989&atitle=Analysis+of+lens+cell+fate+and+eye+morphogenesis+in+transgenic+mice+ablated+for+cells+of+the+lens+lineage-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid2598819en_HK
dc.identifier.scopuseid_2-s2.0-0024362876en_HK
dc.identifier.volume106en_HK
dc.identifier.issue3en_HK
dc.identifier.spage457en_HK
dc.identifier.epage463en_HK
dc.identifier.isiWOS:A1989AF20300005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK
dc.identifier.scopusauthoridBryce, DM=7006138663en_HK
dc.identifier.scopusauthoridGiddens, E=6603893129en_HK
dc.identifier.scopusauthoridClapoff, S=6508066159en_HK
dc.identifier.scopusauthoridGoring, D=7005468517en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridKlintworth, GK=7006444158en_HK
dc.identifier.scopusauthoridBernstein, A=35463110000en_HK

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