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Article: Analysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
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TitleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
 
AuthorsBreitman, ML1
Bryce, DM1
Giddens, E1
Clapoff, S1
Goring, D1
Tsui, LC1
Klintworth, GK1
Bernstein, A1
 
Issue Date1989
 
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
 
CitationDevelopment, 1989, v. 106 n. 3, p. 457-463 [How to Cite?]
 
AbstractTransgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.
 
ISSN0950-1991
2013 Impact Factor: 6.273
 
ISI Accession Number IDWOS:A1989AF20300005
 
DC FieldValue
dc.contributor.authorBreitman, ML
 
dc.contributor.authorBryce, DM
 
dc.contributor.authorGiddens, E
 
dc.contributor.authorClapoff, S
 
dc.contributor.authorGoring, D
 
dc.contributor.authorTsui, LC
 
dc.contributor.authorKlintworth, GK
 
dc.contributor.authorBernstein, A
 
dc.date.accessioned2011-06-01T08:20:15Z
 
dc.date.available2011-06-01T08:20:15Z
 
dc.date.issued1989
 
dc.description.abstractTransgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical γ2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of γ-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationDevelopment, 1989, v. 106 n. 3, p. 457-463 [How to Cite?]
 
dc.identifier.epage463
 
dc.identifier.isiWOS:A1989AF20300005
 
dc.identifier.issn0950-1991
2013 Impact Factor: 6.273
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid2598819
 
dc.identifier.scopuseid_2-s2.0-0024362876
 
dc.identifier.spage457
 
dc.identifier.urihttp://hdl.handle.net/10722/133867
 
dc.identifier.volume106
 
dc.languageeng
 
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofDevelopment
 
dc.subject.meshCrystallins - genetics
 
dc.subject.meshDNA - genetics
 
dc.subject.meshGene Expression
 
dc.subject.meshLens, Crystalline - abnormalities - metabolism - pathology
 
dc.subject.meshMicrophthalmos - genetics
 
dc.titleAnalysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage
 
dc.typeArticle
 
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<contributor.author>Clapoff, S</contributor.author>
<contributor.author>Goring, D</contributor.author>
<contributor.author>Tsui, LC</contributor.author>
<contributor.author>Klintworth, GK</contributor.author>
<contributor.author>Bernstein, A</contributor.author>
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<description.abstract>Transgenic mice carrying the diphtheria toxin A gene driven by mouse &#947;2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical &#947;2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of &#947;-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contribution of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.</description.abstract>
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Author Affiliations
  1. Mount Sinai Hospital, Toronto