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Article: Molecular detection of anaerobic ammonium-oxidizing (Anammox) bacteria in high-temperature petroleum reservoirs

TitleMolecular detection of anaerobic ammonium-oxidizing (Anammox) bacteria in high-temperature petroleum reservoirs
Authors
Issue Date2010
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htm
Citation
Microbial Ecology, 2010, v. 60 n. 4, p. 771-783 How to Cite?
AbstractAnaerobic ammonium-oxidizing (anammox) process plays an important role in the nitrogen cycle of the worldwide anoxic and mesophilic habitats. Recently, the existence and activity of anammox bacteria have been detected in some thermophilic environments, but their existence in the geothermal subterranean oil reservoirs is still not reported. This study investigated the abundance, distribution and functional diversity of anammox bacteria in nine out of 17 high-temperature oil reservoirs by molecular ecology analysis. High concentration (5.31-39.2 mg l-1) of ammonium was detected in the production water from these oilfields with temperatures between 55°C and 75°C. Both 16S rRNA and hzo molecular biomarkers indicated the occurrence of anammox bacteria in nine out of 17 samples. Most of 16S rRNA gene phylotypes are closely related to the known anammox bacterial genera Candidatus Brocadia, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia, while hzo gene phylotypes are closely related to the genera Candidatus Anammoxoglobus, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia. The total bacterial and anammox bacterial densities were 6.4 ± 0.5 × 103 to 2.0 ± 0.18 × 106 cells ml-1 and 6.6 ± 0.51 × 102 to 4.9 ± 0.36 × 104 cell ml-1, respectively. The cluster I of 16S rRNA gene sequences showed distant identity (<92%) to the known Candidatus Scalindua species, inferring this cluster of anammox bacteria to be a new species, and a tentative name Candidatus "Scalindua sinooilfield" was proposed. The results extended the existence of anammox bacteria to the high-temperature oil reservoirs. © 2010 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/133816
ISSN
2015 Impact Factor: 3.232
2015 SCImago Journal Rankings: 1.346
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Environmental Toxicology Education Fund
National Natural Science Foundation of China50744016
National High Technology Research and Development Program of China2009AA063503
China Postdoctoral Science Foundation20080440082
200902223
Shanghai Municipal Science and Technology Commission071607014
Shanghai Postdoctoral Scientific Program09R21411900
Funding Information:

This study was supported by Environmental Toxicology Education Fund (to J-D G), National Natural Science Foundation of China (50744016), National High Technology Research and Development Program of China (2009AA063503), China Postdoctoral Science Foundation funded project (20080440082, 200902223), Shanghai Municipal Science and Technology Commission (071607014), and Shanghai Postdoctoral Scientific Program (09R21411900). We thank Jessie Lai for laboratory assistance and Meng Li for useful discussion.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_HK
dc.contributor.authorChen, Sen_HK
dc.contributor.authorMu, BZen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2011-05-31T07:11:24Z-
dc.date.available2011-05-31T07:11:24Z-
dc.date.issued2010en_HK
dc.identifier.citationMicrobial Ecology, 2010, v. 60 n. 4, p. 771-783en_HK
dc.identifier.issn0095-3628en_HK
dc.identifier.urihttp://hdl.handle.net/10722/133816-
dc.description.abstractAnaerobic ammonium-oxidizing (anammox) process plays an important role in the nitrogen cycle of the worldwide anoxic and mesophilic habitats. Recently, the existence and activity of anammox bacteria have been detected in some thermophilic environments, but their existence in the geothermal subterranean oil reservoirs is still not reported. This study investigated the abundance, distribution and functional diversity of anammox bacteria in nine out of 17 high-temperature oil reservoirs by molecular ecology analysis. High concentration (5.31-39.2 mg l-1) of ammonium was detected in the production water from these oilfields with temperatures between 55°C and 75°C. Both 16S rRNA and hzo molecular biomarkers indicated the occurrence of anammox bacteria in nine out of 17 samples. Most of 16S rRNA gene phylotypes are closely related to the known anammox bacterial genera Candidatus Brocadia, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia, while hzo gene phylotypes are closely related to the genera Candidatus Anammoxoglobus, Candidatus Kuenenia, Candidatus Scalindua, and Candidatus Jettenia. The total bacterial and anammox bacterial densities were 6.4 ± 0.5 × 103 to 2.0 ± 0.18 × 106 cells ml-1 and 6.6 ± 0.51 × 102 to 4.9 ± 0.36 × 104 cell ml-1, respectively. The cluster I of 16S rRNA gene sequences showed distant identity (<92%) to the known Candidatus Scalindua species, inferring this cluster of anammox bacteria to be a new species, and a tentative name Candidatus "Scalindua sinooilfield" was proposed. The results extended the existence of anammox bacteria to the high-temperature oil reservoirs. © 2010 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htmen_HK
dc.relation.ispartofMicrobial Ecologyen_HK
dc.rightsThe original publication is available at www.springerlink.comen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacteria - classification - genetics - isolation and purification - metabolism-
dc.subject.meshBiodiversity-
dc.subject.meshDNA, Bacterial - genetics-
dc.subject.meshPetroleum - microbiology-
dc.subject.meshQuaternary Ammonium Compounds - metabolism-
dc.titleMolecular detection of anaerobic ammonium-oxidizing (Anammox) bacteria in high-temperature petroleum reservoirsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-3628&volume=60&issue=4&spage=771&epage=783&date=2010&atitle=Molecular+detection+of+Anaerobic+ammonium-oxidizing+(anammox)+bacteria+in+high+temperature+petroleum+reservoirs-
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1007/s00248-010-9733-3en_HK
dc.identifier.pmid20740282-
dc.identifier.pmcidPMC2974184-
dc.identifier.scopuseid_2-s2.0-78149471109en_HK
dc.identifier.hkuros194832-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78149471109&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue4en_HK
dc.identifier.spage771en_HK
dc.identifier.epage783en_HK
dc.identifier.eissn1432-184Xen_US
dc.identifier.isiWOS:000284255700008-
dc.publisher.placeUnited Statesen_HK
dc.description.otherSpringer Open Choice, 31 May 2011en_US
dc.identifier.scopusauthoridLi, H=36077120500en_HK
dc.identifier.scopusauthoridChen, S=37037039300en_HK
dc.identifier.scopusauthoridMu, BZ=7004585949en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.citeulike7773707-

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