Article: Novel immunomodulatory effects of adiponectin on dendritic cell functions

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TitleNovel immunomodulatory effects of adiponectin on dendritic cell functions
AuthorsTsang, JYS1
Li, D1
Ho, D1
Peng, J1
Xu, A1
Lamb, J2
Chen, Y1
Tam, PKH1
KeywordsAdiponectin
Dendritic cell
Immunomodulation
Programmed death-1 ligand
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimp
CitationInternational Immunopharmacology, 2011, v. 11 n. 5, p. 604-609 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.intimp.2010.11.009
AbstractAdiponectin (ADN) is an adipocytokine with anti-inflammatory properties. Although it has been reported that ADN can inhibit the immunostimulatory function of monocytes and macrophages, little is known of its effect on dendritic cells (DC). Recent data suggest that ADN can regulate immune responses. DCs are uniquely specialised antigen presenting cells that play a central role in the initiation of immunity and tolerance. In this study, we have investigated the immuno- modulatory effects of ADN on DC functions. We found that ADN has only moderate effect on the differentiation of murine bone marrow (BM) derived DCs but altered the phenotype of DCs. The expression of major histocompatibilty complex class II (MHCII), CD80 and CD86 on ADN conditioned DCs (ADN-DCs) was lower than that on untreated cells. The production of IL-12p40 was also suppressed in ADN-DCs. Interestingly, ADN treated DCs showed an increase in the expression of the inhibitory molecule, programmed death-1 ligand (PDL-1) compared to untreated cells. In vitro co-culture of ADN-DCs with allogeneic T cells led to a decrease in T cell proliferation and reduction of IL-2 production. Concomitant with that, a higher percentage of CD4 +CD25 +Foxp3 + regulatory T cells (Tregs) was detected in co-cultures of T cells and ADN-DCs. Blocking PD-1/PDL-1 pathway could partially restore T cell function. These findings suggest that the immunomodulatory effect of ADN on immune responses could be at least partially be mediated by its ability to alter DC function. The PD-1/PDL-1 pathway and the enhancement of Treg expansion are implicated in the immunomodulatory mechanisms. © 2010 Elsevier B.V.
ISSN1567-5769
2011 Impact Factor: 2.376
2011 SCImago Journal Rankings: 0.188
DOIhttp://dx.doi.org/10.1016/j.intimp.2010.11.009
ISI Accession Number IDWOS:000291069700012
Funding AgencyGrant Number
HKU762108M
Funding Information:

This work is supported by research grant from the Hong Kong General Research Fund (HKU 762108M).

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTsang, JYS
dc.contributor.authorLi, D
dc.contributor.authorHo, D
dc.contributor.authorPeng, J
dc.contributor.authorXu, A
dc.contributor.authorLamb, J
dc.contributor.authorChen, Y
dc.contributor.authorTam, PKH
dc.date.accessioned2011-05-24T02:13:40Z
dc.date.available2011-05-24T02:13:40Z
dc.date.issued2011
dc.description.abstractAdiponectin (ADN) is an adipocytokine with anti-inflammatory properties. Although it has been reported that ADN can inhibit the immunostimulatory function of monocytes and macrophages, little is known of its effect on dendritic cells (DC). Recent data suggest that ADN can regulate immune responses. DCs are uniquely specialised antigen presenting cells that play a central role in the initiation of immunity and tolerance. In this study, we have investigated the immuno- modulatory effects of ADN on DC functions. We found that ADN has only moderate effect on the differentiation of murine bone marrow (BM) derived DCs but altered the phenotype of DCs. The expression of major histocompatibilty complex class II (MHCII), CD80 and CD86 on ADN conditioned DCs (ADN-DCs) was lower than that on untreated cells. The production of IL-12p40 was also suppressed in ADN-DCs. Interestingly, ADN treated DCs showed an increase in the expression of the inhibitory molecule, programmed death-1 ligand (PDL-1) compared to untreated cells. In vitro co-culture of ADN-DCs with allogeneic T cells led to a decrease in T cell proliferation and reduction of IL-2 production. Concomitant with that, a higher percentage of CD4 +CD25 +Foxp3 + regulatory T cells (Tregs) was detected in co-cultures of T cells and ADN-DCs. Blocking PD-1/PDL-1 pathway could partially restore T cell function. These findings suggest that the immunomodulatory effect of ADN on immune responses could be at least partially be mediated by its ability to alter DC function. The PD-1/PDL-1 pathway and the enhancement of Treg expansion are implicated in the immunomodulatory mechanisms. © 2010 Elsevier B.V.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationInternational Immunopharmacology, 2011, v. 11 n. 5, p. 604-609 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.intimp.2010.11.009
dc.identifier.citeulike8366256
dc.identifier.doihttp://dx.doi.org/10.1016/j.intimp.2010.11.009
dc.identifier.epage609
dc.identifier.hkuros185390
dc.identifier.isiWOS:000291069700012
Funding AgencyGrant Number
HKU762108M
Funding Information:

This work is supported by research grant from the Hong Kong General Research Fund (HKU 762108M).

dc.identifier.issn1567-5769
2011 Impact Factor: 2.376
2011 SCImago Journal Rankings: 0.188
dc.identifier.issue5
dc.identifier.openurl
dc.identifier.pmid21094289
dc.identifier.scopuseid_2-s2.0-79954631240
dc.identifier.spage604
dc.identifier.urihttp://hdl.handle.net/10722/133655
dc.identifier.volume11
dc.languageeng
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimp
dc.publisher.placeUnited Kingdom
dc.relation.ispartofInternational Immunopharmacology
dc.relation.referencesReferences in Scopus
dc.subjectAdiponectin
dc.subjectDendritic cell
dc.subjectImmunomodulation
dc.subjectProgrammed death-1 ligand
dc.titleNovel immunomodulatory effects of adiponectin on dendritic cell functions
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Imperial College London