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Article: Novel immunomodulatory effects of adiponectin on dendritic cell functions
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TitleNovel immunomodulatory effects of adiponectin on dendritic cell functions
 
AuthorsTsang, JYS1
Li, D1
Ho, D1
Peng, J1
Xu, A1
Lamb, J2
Chen, Y1
Tam, PKH1
 
KeywordsAdiponectin
Dendritic cell
Immunomodulation
Programmed death-1 ligand
 
Issue Date2011
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimp
 
CitationInternational Immunopharmacology, 2011, v. 11 n. 5, p. 604-609 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.intimp.2010.11.009
 
AbstractAdiponectin (ADN) is an adipocytokine with anti-inflammatory properties. Although it has been reported that ADN can inhibit the immunostimulatory function of monocytes and macrophages, little is known of its effect on dendritic cells (DC). Recent data suggest that ADN can regulate immune responses. DCs are uniquely specialised antigen presenting cells that play a central role in the initiation of immunity and tolerance. In this study, we have investigated the immuno- modulatory effects of ADN on DC functions. We found that ADN has only moderate effect on the differentiation of murine bone marrow (BM) derived DCs but altered the phenotype of DCs. The expression of major histocompatibilty complex class II (MHCII), CD80 and CD86 on ADN conditioned DCs (ADN-DCs) was lower than that on untreated cells. The production of IL-12p40 was also suppressed in ADN-DCs. Interestingly, ADN treated DCs showed an increase in the expression of the inhibitory molecule, programmed death-1 ligand (PDL-1) compared to untreated cells. In vitro co-culture of ADN-DCs with allogeneic T cells led to a decrease in T cell proliferation and reduction of IL-2 production. Concomitant with that, a higher percentage of CD4 +CD25 +Foxp3 + regulatory T cells (Tregs) was detected in co-cultures of T cells and ADN-DCs. Blocking PD-1/PDL-1 pathway could partially restore T cell function. These findings suggest that the immunomodulatory effect of ADN on immune responses could be at least partially be mediated by its ability to alter DC function. The PD-1/PDL-1 pathway and the enhancement of Treg expansion are implicated in the immunomodulatory mechanisms. © 2010 Elsevier B.V.
 
ISSN1567-5769
2013 Impact Factor: 2.711
2013 SCImago Journal Rankings: 0.970
 
DOIhttp://dx.doi.org/10.1016/j.intimp.2010.11.009
 
ISI Accession Number IDWOS:000291069700012
Funding AgencyGrant Number
HKU762108M
Funding Information:

This work is supported by research grant from the Hong Kong General Research Fund (HKU 762108M).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTsang, JYS
 
dc.contributor.authorLi, D
 
dc.contributor.authorHo, D
 
dc.contributor.authorPeng, J
 
dc.contributor.authorXu, A
 
dc.contributor.authorLamb, J
 
dc.contributor.authorChen, Y
 
dc.contributor.authorTam, PKH
 
dc.date.accessioned2011-05-24T02:13:40Z
 
dc.date.available2011-05-24T02:13:40Z
 
dc.date.issued2011
 
dc.description.abstractAdiponectin (ADN) is an adipocytokine with anti-inflammatory properties. Although it has been reported that ADN can inhibit the immunostimulatory function of monocytes and macrophages, little is known of its effect on dendritic cells (DC). Recent data suggest that ADN can regulate immune responses. DCs are uniquely specialised antigen presenting cells that play a central role in the initiation of immunity and tolerance. In this study, we have investigated the immuno- modulatory effects of ADN on DC functions. We found that ADN has only moderate effect on the differentiation of murine bone marrow (BM) derived DCs but altered the phenotype of DCs. The expression of major histocompatibilty complex class II (MHCII), CD80 and CD86 on ADN conditioned DCs (ADN-DCs) was lower than that on untreated cells. The production of IL-12p40 was also suppressed in ADN-DCs. Interestingly, ADN treated DCs showed an increase in the expression of the inhibitory molecule, programmed death-1 ligand (PDL-1) compared to untreated cells. In vitro co-culture of ADN-DCs with allogeneic T cells led to a decrease in T cell proliferation and reduction of IL-2 production. Concomitant with that, a higher percentage of CD4 +CD25 +Foxp3 + regulatory T cells (Tregs) was detected in co-cultures of T cells and ADN-DCs. Blocking PD-1/PDL-1 pathway could partially restore T cell function. These findings suggest that the immunomodulatory effect of ADN on immune responses could be at least partially be mediated by its ability to alter DC function. The PD-1/PDL-1 pathway and the enhancement of Treg expansion are implicated in the immunomodulatory mechanisms. © 2010 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationInternational Immunopharmacology, 2011, v. 11 n. 5, p. 604-609 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.intimp.2010.11.009
 
dc.identifier.citeulike8366256
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.intimp.2010.11.009
 
dc.identifier.epage609
 
dc.identifier.hkuros185390
 
dc.identifier.isiWOS:000291069700012
Funding AgencyGrant Number
HKU762108M
Funding Information:

This work is supported by research grant from the Hong Kong General Research Fund (HKU 762108M).

 
dc.identifier.issn1567-5769
2013 Impact Factor: 2.711
2013 SCImago Journal Rankings: 0.970
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmid21094289
 
dc.identifier.scopuseid_2-s2.0-79954631240
 
dc.identifier.spage604
 
dc.identifier.urihttp://hdl.handle.net/10722/133655
 
dc.identifier.volume11
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimp
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofInternational Immunopharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAdiponectin
 
dc.subjectDendritic cell
 
dc.subjectImmunomodulation
 
dc.subjectProgrammed death-1 ligand
 
dc.titleNovel immunomodulatory effects of adiponectin on dendritic cell functions
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Imperial College London