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Article: Angiotensin II induces interleukin-6 synthesis in osteoblasts through ERK1/2 pathway via AT1 receptor
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TitleAngiotensin II induces interleukin-6 synthesis in osteoblasts through ERK1/2 pathway via AT1 receptor
 
AuthorsGuo, L2
Wang, M2
Zhang, ZY2
Hao, L2
Lou, BY2
Li, XY2
Loo, WTY1
Jin, L1
Cheung, MNB1
 
KeywordsAngiotensin II
Extracellular signal-regulated kinase
Interleukin-6
Osteoblast
 
Issue Date2011
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
 
CitationArchives Of Oral Biology, 2011, v. 56 n. 3, p. 205-211 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.archoralbio.2010.09.016
 
AbstractBackground: Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating endothelia cells, vascular smooth muscle cells (VSMC) and monocytes. However, of the mechanism by which Ang II regulates IL-6 expression in osteoblasts is unknown. Aims: The present study was designed to investigate the effect of Ang II on IL-6 expression in osteoblasts isolated from mice. The receptor(s) required and the potential role of extracellular signal-regulated kinase 1/2 (ERK1/2) activation in Ang II-induced IL-6 synthesis was also examined in these cells. Methods: The osteoblasts were isolated from the calvaria of mice and cultured in α-MEM medium. IL-6 mRNA expression and protein synthesis was determined by qPCR and ELISA analyses. ERK1/2 kinase activation was determined by western blot. Results: The results indicate that Ang II induced IL-6 mRNA expression and protein synthesis in cultured osteoblasts. However, these effects were abolished by pre-treatment with Ang II type 1 (AT1) receptor antagonist, losartan, and the ERK1/2 inhibitor, U0126, inhibited Ang II-mediated IL-6 expression and the phosphorylation of ERK1/2. Conclusion: Ang II induces the synthesis of IL-6 in osteoblasts through activation of the ERK1/2 pathway via the AT1 receptor. Crown Copyright © 2010 Published by Elsevier Ltd. All rights reserved.
 
ISSN0003-9969
2012 Impact Factor: 1.549
2012 SCImago Journal Rankings: 0.475
 
DOIhttp://dx.doi.org/10.1016/j.archoralbio.2010.09.016
 
ISI Accession Number IDWOS:000288474400001
Funding AgencyGrant Number
Public Welfare Foundation of the Sichuan Science and Technology Department of China2008FN0174
Funding Information:

This study was supported by the Public Welfare Foundation of the Sichuan Science and Technology Department of China (No. 2008FN0174).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGuo, L
 
dc.contributor.authorWang, M
 
dc.contributor.authorZhang, ZY
 
dc.contributor.authorHao, L
 
dc.contributor.authorLou, BY
 
dc.contributor.authorLi, XY
 
dc.contributor.authorLoo, WTY
 
dc.contributor.authorJin, L
 
dc.contributor.authorCheung, MNB
 
dc.date.accessioned2011-05-24T02:10:46Z
 
dc.date.available2011-05-24T02:10:46Z
 
dc.date.issued2011
 
dc.description.abstractBackground: Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating endothelia cells, vascular smooth muscle cells (VSMC) and monocytes. However, of the mechanism by which Ang II regulates IL-6 expression in osteoblasts is unknown. Aims: The present study was designed to investigate the effect of Ang II on IL-6 expression in osteoblasts isolated from mice. The receptor(s) required and the potential role of extracellular signal-regulated kinase 1/2 (ERK1/2) activation in Ang II-induced IL-6 synthesis was also examined in these cells. Methods: The osteoblasts were isolated from the calvaria of mice and cultured in α-MEM medium. IL-6 mRNA expression and protein synthesis was determined by qPCR and ELISA analyses. ERK1/2 kinase activation was determined by western blot. Results: The results indicate that Ang II induced IL-6 mRNA expression and protein synthesis in cultured osteoblasts. However, these effects were abolished by pre-treatment with Ang II type 1 (AT1) receptor antagonist, losartan, and the ERK1/2 inhibitor, U0126, inhibited Ang II-mediated IL-6 expression and the phosphorylation of ERK1/2. Conclusion: Ang II induces the synthesis of IL-6 in osteoblasts through activation of the ERK1/2 pathway via the AT1 receptor. Crown Copyright © 2010 Published by Elsevier Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationArchives Of Oral Biology, 2011, v. 56 n. 3, p. 205-211 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.archoralbio.2010.09.016
 
dc.identifier.citeulike8366099
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.archoralbio.2010.09.016
 
dc.identifier.epage211
 
dc.identifier.hkuros185238
 
dc.identifier.isiWOS:000288474400001
Funding AgencyGrant Number
Public Welfare Foundation of the Sichuan Science and Technology Department of China2008FN0174
Funding Information:

This study was supported by the Public Welfare Foundation of the Sichuan Science and Technology Department of China (No. 2008FN0174).

 
dc.identifier.issn0003-9969
2012 Impact Factor: 1.549
2012 SCImago Journal Rankings: 0.475
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid21109230
 
dc.identifier.scopuseid_2-s2.0-79951855395
 
dc.identifier.spage205
 
dc.identifier.urihttp://hdl.handle.net/10722/133570
 
dc.identifier.volume56
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofArchives of Oral Biology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAngiotensin II - physiology
 
dc.subject.meshInterleukin-6 - biosynthesis - secretion
 
dc.subject.meshMitogen-Activated Protein Kinase 1 - antagonists and inhibitors - metabolism
 
dc.subject.meshMitogen-Activated Protein Kinase 3 - antagonists and inhibitors - metabolism
 
dc.subject.meshOsteoblasts - metabolism
 
dc.subjectAngiotensin II
 
dc.subjectExtracellular signal-regulated kinase
 
dc.subjectInterleukin-6
 
dc.subjectOsteoblast
 
dc.titleAngiotensin II induces interleukin-6 synthesis in osteoblasts through ERK1/2 pathway via AT1 receptor
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Sichuan University