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Article: Polymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study (in Chinese)

TitlePolymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study (in Chinese)
廣州生物庫隊列人群心腦血管病相關基因多態性研究
Authors
Issue Date2010
PublisherChinese Medical Association. The Journal's web site is located at http://zhyfyx.periodicals.net.cn/
Citation
中華預防醫學雜誌, 2010, v. 44 n. 1, p. 65-69 How to Cite?
Chinese Journal of Preventive Medicine, 2010, v. 44 n. 1, p. 65-69 How to Cite?
AbstractOBJECTIVE: To study the polymorphisms of cerebrovascular and cardiovascular disease genes using Taqman single nucleotide polymorphism (SNP) genotyping kits. METHODS: A total of 2000 subjects were recruited from the Guangzhou Biobank Cohort Study (GBCS), and 15 SNPs were detected using Taqman SNP genotyping kits and an ABI 7900HT real time PCR system. The data were tested for the Hardy-Weinberg equilibrium, and then compared with the data of the Chinese population from the International HapMap Project (HapMap_HCN). RESULTS: (1) All genotype data of the 15 SNPs were consistent with the Hardy-Weinberg rules. (2) The significant differences were observed among two SNPs, rs4220 and rs5368 and the HapMap_HCN (rs4220 28.2% vs 17.8%; chi(2) = 4.891, P = 0.028; rs5368 22.1% vs 32.2%, chi(2) = 5.137, P = 0.024). Comparing other gene bank data, such as AFD-CHN-PANEL, the Allele Frequency Database (ALFRED) and JBIC-allele, it would be most likely that our observations represent differences between the Northern and Southern populations in China. CONCLUSION: Such Biobank study provided a useful platform for the study of the role of genetic and environmental determinants on cerebrovascular and cardiovascular disease. 目的 應用Taqman單核苷酸多態性(SNP)分型法檢測廣州生物庫隊列人群心腦血管疾病相關基因的多態性.方法 從廣州生物庫隊列研究(GBCS)中選取2000名研究對象,應用ABI7900HT型熒光定最PCR儀并采用Taqman SNP分型法檢測該人群rs4220、rs5368及rs285等15個心腦血管疾病相關基因多態性.對檢測的基因分型數據采用遺傳平衡定律檢測,并與國際染色體單倍型計劃(HapMap)的中國人群數據進行比較.結果 (1)所有15個SNP的分型數據均滿足遺傳平衡定律;(2)有兩個SNP的分型數據與HapMap的中國人群數據差異有統計學意義(rs4220 28.2%對17.8%,X2=4.891,P=0.028;rs5368 22.1%對32.2%,X2=5.137,P=0.024).對比美國Perlegen公司人類遺傳變異研究(AFD-CHN-PANEL)、美國科學基金會等位基因頻率數據庫(ALFRED)、日本生物信息學協會(JBIC-allele)等多個基因庫數據,造成本研究SNP頻率不同于HapMup的中國人群數據的原因是華南人群在遺傳背景上與北方人群有一定的差異.結論本研究的數據可能更能反映南方人的特性,因此,建立大樣本的廣州人群心腦血管疾病相關基因多態性數據庫以研究基因與環境對心腦血管疾病的影響是必要的.
Persistent Identifierhttp://hdl.handle.net/10722/133334
ISSN
2015 SCImago Journal Rankings: 0.236

 

DC FieldValueLanguage
dc.contributor.authorLiu, Ben_US
dc.contributor.authorJiang, CQen_US
dc.contributor.authorThomas, GNen_US
dc.contributor.authorLao, Xen_US
dc.contributor.authorLin, JMen_US
dc.contributor.authorYue, XJen_US
dc.contributor.authorZhang, WSen_US
dc.contributor.authorZhu, Ten_US
dc.contributor.authorLam, THen_US
dc.date.accessioned2011-05-11T08:32:18Z-
dc.date.available2011-05-11T08:32:18Z-
dc.date.issued2010en_US
dc.identifier.citation中華預防醫學雜誌, 2010, v. 44 n. 1, p. 65-69zh_HK
dc.identifier.citationChinese Journal of Preventive Medicine, 2010, v. 44 n. 1, p. 65-69-
dc.identifier.issn0253-9624-
dc.identifier.urihttp://hdl.handle.net/10722/133334-
dc.description.abstractOBJECTIVE: To study the polymorphisms of cerebrovascular and cardiovascular disease genes using Taqman single nucleotide polymorphism (SNP) genotyping kits. METHODS: A total of 2000 subjects were recruited from the Guangzhou Biobank Cohort Study (GBCS), and 15 SNPs were detected using Taqman SNP genotyping kits and an ABI 7900HT real time PCR system. The data were tested for the Hardy-Weinberg equilibrium, and then compared with the data of the Chinese population from the International HapMap Project (HapMap_HCN). RESULTS: (1) All genotype data of the 15 SNPs were consistent with the Hardy-Weinberg rules. (2) The significant differences were observed among two SNPs, rs4220 and rs5368 and the HapMap_HCN (rs4220 28.2% vs 17.8%; chi(2) = 4.891, P = 0.028; rs5368 22.1% vs 32.2%, chi(2) = 5.137, P = 0.024). Comparing other gene bank data, such as AFD-CHN-PANEL, the Allele Frequency Database (ALFRED) and JBIC-allele, it would be most likely that our observations represent differences between the Northern and Southern populations in China. CONCLUSION: Such Biobank study provided a useful platform for the study of the role of genetic and environmental determinants on cerebrovascular and cardiovascular disease. 目的 應用Taqman單核苷酸多態性(SNP)分型法檢測廣州生物庫隊列人群心腦血管疾病相關基因的多態性.方法 從廣州生物庫隊列研究(GBCS)中選取2000名研究對象,應用ABI7900HT型熒光定最PCR儀并采用Taqman SNP分型法檢測該人群rs4220、rs5368及rs285等15個心腦血管疾病相關基因多態性.對檢測的基因分型數據采用遺傳平衡定律檢測,并與國際染色體單倍型計劃(HapMap)的中國人群數據進行比較.結果 (1)所有15個SNP的分型數據均滿足遺傳平衡定律;(2)有兩個SNP的分型數據與HapMap的中國人群數據差異有統計學意義(rs4220 28.2%對17.8%,X2=4.891,P=0.028;rs5368 22.1%對32.2%,X2=5.137,P=0.024).對比美國Perlegen公司人類遺傳變異研究(AFD-CHN-PANEL)、美國科學基金會等位基因頻率數據庫(ALFRED)、日本生物信息學協會(JBIC-allele)等多個基因庫數據,造成本研究SNP頻率不同于HapMup的中國人群數據的原因是華南人群在遺傳背景上與北方人群有一定的差異.結論本研究的數據可能更能反映南方人的特性,因此,建立大樣本的廣州人群心腦血管疾病相關基因多態性數據庫以研究基因與環境對心腦血管疾病的影響是必要的.zh_HK
dc.languagechien_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://zhyfyx.periodicals.net.cn/-
dc.relation.ispartof中華預防醫學雜誌zh_HK
dc.relation.ispartofChinese Journal of Preventive Medicine-
dc.subject.meshBiological Specimen Banks - statistics and numerical data-
dc.subject.meshBrain Diseases - epidemiology - genetics-
dc.subject.meshCardiovascular Diseases - epidemiology - genetics-
dc.subject.meshChina - epidemiology-
dc.subject.meshPolymorphism, Single Nucleotide-
dc.titlePolymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study (in Chinese)en_US
dc.title廣州生物庫隊列人群心腦血管病相關基因多態性研究zh_HK
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0253-9624&volume=44&issue=1&spage=65&epage=69&date=2010&atitle=Polymorphisms+of+vascular+diseases-related+genes+in+Guangzhou+Biobank+Cohort+Study+(in+Chinese)-
dc.identifier.emailJiang, CQ: cqjiang@HKUCC.hku.hken_US
dc.identifier.emailThomas, GN: neilt@HKUCC.hku.hken_US
dc.identifier.emailLao, X: xqlao@hkucc.hku.hken_US
dc.identifier.emailZhang, WS: zhangws9@HKUCC-COM.hku.hken_US
dc.identifier.emailLam, TH: hrmrlth@hkucc.hku.hken_US
dc.identifier.authorityLam, TH=rp00326en_US
dc.identifier.doi10.3760/cma.j.issn.0253-9624.2010.01.016-
dc.identifier.pmid20388367-
dc.identifier.hkuros184771en_US
dc.identifier.volume44en_US
dc.identifier.issue1en_US
dc.identifier.spage65en_US
dc.identifier.epage69en_US

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