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Conference Paper: Effects of Chinese green tea on cigarette smoke-induced lung inflammation, oxidative stress and protease activity in rats

TitleEffects of Chinese green tea on cigarette smoke-induced lung inflammation, oxidative stress and protease activity in rats
Authors
KeywordsMedical sciences
Respiratory diseases
Issue Date2010
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
International Conference of the American Thoracic Society (ATS) 2009, San Diego, CA., 15-20 May 2010. In American Journal of Respiratory and Critical Care Medicine, 2010, v. 181 (Meeting abstracts), no. A5062 How to Cite?
AbstractINTRODUCTION: Chronic obstructive pulmonary disease (COPD) is a progressively destructive airway disease mainly caused by smoking. Systemic and lung inflammation persists in COPD patients even after smoking cessation. Chronic inflammation and/or oxidative stress cause lung damage due to the release of destructive proteases such as matrix metalloproteinases (MMPs)-2/9/12. Chinese green tea is thought to have both anti-inflammatory and anti-oxidative effects in different systems. This study is to address whether cigarette smoke (CS) exposure would induce lung inflammation, oxidative stress and MMPs activity, and whether Chinese green tea would reverse such effects. METHODS: Sprague-Dawley rats were divided into four groups, i.e. sham air (SA), 4% CS, 2% Lung Chen tea plus SA or 4% CS. Exposure to sham air or 4% CS was performed for 1h/day for 56 days in ventilated smoking chambers. Lung tissues and bronchoalveolar lavage fluid (BALF) were obtained from rats sacrificed 24h after last CS exposure. The protein levels of pro-inflammatory cytokines/chemokines were determined by ELISA. The MMP activity was assessed by gelatin and casein zymography respectively. Local oxidative damage was determined by staining lung sections with anti-8-oxo-dG antibody and the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) in lung homogenates. RESULTS: Elevation of interleukin (IL)-1b/-6, interferon (IFN)-g and cytokine induced neutrophil chemoattractant (CINC)-1 was observed in lung after CS exposure. MMP-12 activity was increased in BALF in CS-exposed rats. There were no differences between SA or CS exposure in MMP-2/-9 activities in BALF. Pretreatment of Chinese green tea reversed local DNA oxidation and IL-6 elevation (1.48 ± 0.25 vs 0.67 ± 0.14 ng/mg protein, for CS and Tea/CS respectively; p < 0.01) but did not affect other pro-inflammatory cytokines/chemokines and MMP-12 activity. CONCLUSION: Our data demonstrated that cigarette smoke exposure led to lung inflammation, local oxidative stress and elevation of protease activity in our rat model. These results support that Chinese green tea might be beneficial to smoke-induced local oxidative damage and inflammation.
DescriptionSession - C67 Emphysema: Animal Models and Treatment Modalities
Persistent Identifierhttp://hdl.handle.net/10722/133306
ISSN
2015 Impact Factor: 13.118
2015 SCImago Journal Rankings: 5.832

 

DC FieldValueLanguage
dc.contributor.authorChan, KH-
dc.contributor.authorYeung, SC-
dc.contributor.authorIp, MSM-
dc.contributor.authorMan, RYK-
dc.contributor.authorMak, JCW-
dc.date.accessioned2011-05-11T07:18:37Z-
dc.date.available2011-05-11T07:18:37Z-
dc.date.issued2010-
dc.identifier.citationInternational Conference of the American Thoracic Society (ATS) 2009, San Diego, CA., 15-20 May 2010. In American Journal of Respiratory and Critical Care Medicine, 2010, v. 181 (Meeting abstracts), no. A5062-
dc.identifier.issn1073-449X-
dc.identifier.urihttp://hdl.handle.net/10722/133306-
dc.descriptionSession - C67 Emphysema: Animal Models and Treatment Modalities-
dc.description.abstractINTRODUCTION: Chronic obstructive pulmonary disease (COPD) is a progressively destructive airway disease mainly caused by smoking. Systemic and lung inflammation persists in COPD patients even after smoking cessation. Chronic inflammation and/or oxidative stress cause lung damage due to the release of destructive proteases such as matrix metalloproteinases (MMPs)-2/9/12. Chinese green tea is thought to have both anti-inflammatory and anti-oxidative effects in different systems. This study is to address whether cigarette smoke (CS) exposure would induce lung inflammation, oxidative stress and MMPs activity, and whether Chinese green tea would reverse such effects. METHODS: Sprague-Dawley rats were divided into four groups, i.e. sham air (SA), 4% CS, 2% Lung Chen tea plus SA or 4% CS. Exposure to sham air or 4% CS was performed for 1h/day for 56 days in ventilated smoking chambers. Lung tissues and bronchoalveolar lavage fluid (BALF) were obtained from rats sacrificed 24h after last CS exposure. The protein levels of pro-inflammatory cytokines/chemokines were determined by ELISA. The MMP activity was assessed by gelatin and casein zymography respectively. Local oxidative damage was determined by staining lung sections with anti-8-oxo-dG antibody and the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) in lung homogenates. RESULTS: Elevation of interleukin (IL)-1b/-6, interferon (IFN)-g and cytokine induced neutrophil chemoattractant (CINC)-1 was observed in lung after CS exposure. MMP-12 activity was increased in BALF in CS-exposed rats. There were no differences between SA or CS exposure in MMP-2/-9 activities in BALF. Pretreatment of Chinese green tea reversed local DNA oxidation and IL-6 elevation (1.48 ± 0.25 vs 0.67 ± 0.14 ng/mg protein, for CS and Tea/CS respectively; p < 0.01) but did not affect other pro-inflammatory cytokines/chemokines and MMP-12 activity. CONCLUSION: Our data demonstrated that cigarette smoke exposure led to lung inflammation, local oxidative stress and elevation of protease activity in our rat model. These results support that Chinese green tea might be beneficial to smoke-induced local oxidative damage and inflammation.-
dc.languageeng-
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org-
dc.relation.ispartofAmerican Journal of Respiratory and Critical Care Medicine-
dc.subjectMedical sciences-
dc.subjectRespiratory diseases-
dc.titleEffects of Chinese green tea on cigarette smoke-induced lung inflammation, oxidative stress and protease activity in ratsen_US
dc.typeConference_Paperen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1073-449X&volume=181: A5062&spage=&epage=&date=2010&atitle=Effects+of+Chinese+green+tea+on+cigarette+smoke-induced+lung+inflammation,+oxidative+stress+and+protease+activity+in+rats-
dc.identifier.emailChan, KH: b01006975@gmail.com-
dc.identifier.emailYeung, SC: yeungsc@hkucc.hku.hk-
dc.identifier.emailIp, MSM: msmip@hku.hk-
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hk-
dc.identifier.emailMak, JCW: judymak@HKUCC.hku.hk-
dc.identifier.hkuros171677-
dc.identifier.volume181-
dc.identifier.issueMeeting abstracts-
dc.description.otherInternational Conference of the American Thoracic Society (ATS) 2009, San Diego, CA., 15-20 May 2010. In American Journal of Respiratory and Critical Care Medicine, 2010, v. 181 (Meeting abstracts), no. A5062-

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