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Article: Visualization of amylase secretion from individual pancreatic acini

TitleVisualization of amylase secretion from individual pancreatic acini
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1988
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1988, v. 254 n. 5, p. 17/5 How to Cite?
AbstractTo assess the secretion of individual rat pancreatic acini, we developed a reverse hemolytic plaque assay that allows for a direct visualization of amylase release. This release was detected around secreting cells by the presence of hemolytic plaques that resulted from the complement-mediated lysis of red blood cells bearing amylase-antiamylase complexes bound to protein A. Controls showed that these plaques reflected specifically the active secretion of amylase. Quantitation of hemolytic plaques showed that after a 30-min incubation ~50% of the acini secreted under basal conditions. Stimulation of amylase release by increasing concentrations of carbamylcholine resulted in a dose-dependent recruitment of secreting acini as well as in a time-dependent enhancement in the response of individual acini. Under all conditions, the wide distribution of hemolytic plaque sizes indicated large differences in the secretory output of individual acini. Thus, using a new method to directly visualize and quantitate amylase secretion, we have provided evidence for a functional heterogeneity of pancreatic acini.
Persistent Identifierhttp://hdl.handle.net/10722/132780
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBosco, Den_HK
dc.contributor.authorChanson, Men_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorMeda, Pen_HK
dc.date.accessioned2011-03-28T09:28:55Z-
dc.date.available2011-03-28T09:28:55Z-
dc.date.issued1988en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1988, v. 254 n. 5, p. 17/5en_HK
dc.identifier.issn0002-9513en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132780-
dc.description.abstractTo assess the secretion of individual rat pancreatic acini, we developed a reverse hemolytic plaque assay that allows for a direct visualization of amylase release. This release was detected around secreting cells by the presence of hemolytic plaques that resulted from the complement-mediated lysis of red blood cells bearing amylase-antiamylase complexes bound to protein A. Controls showed that these plaques reflected specifically the active secretion of amylase. Quantitation of hemolytic plaques showed that after a 30-min incubation ~50% of the acini secreted under basal conditions. Stimulation of amylase release by increasing concentrations of carbamylcholine resulted in a dose-dependent recruitment of secreting acini as well as in a time-dependent enhancement in the response of individual acini. Under all conditions, the wide distribution of hemolytic plaque sizes indicated large differences in the secretory output of individual acini. Thus, using a new method to directly visualize and quantitate amylase secretion, we have provided evidence for a functional heterogeneity of pancreatic acini.en_HK
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.titleVisualization of amylase secretion from individual pancreatic acinien_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2452575-
dc.identifier.scopuseid_2-s2.0-0023914458en_HK
dc.identifier.volume254en_HK
dc.identifier.issue5en_HK
dc.identifier.spage17/5en_HK
dc.identifier.epage17/5en_HK
dc.identifier.isiWOS:A1988N390700042-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridBosco, D=7005328418en_HK
dc.identifier.scopusauthoridChanson, M=7004131733en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridMeda, P=7005822187en_HK

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