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Article: Cell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue

TitleCell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue
Authors
Issue Date1988
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1988, v. 255 n. 5, p. 24/5 How to Cite?
AbstractSecond messengers have been implicated in the control of communication between cells of various tissues and of a number of cell lines. To assess whether protein kinase C (PKC) is involved in the regulation of gap junctions between primary differentiated cells, we studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on PKC translocation and junctional conductance of rat pancreatic exocrine cells. Our results show that although TPA induced the translocation of PKC from a 'cytosolic' to a 'microsomal' fraction within minutes, it failed to block the junctional conductance of acinar cell pairs up to 30 min after application. By contrast, analogous experiments on a liver-derived cell line (WB cells) showed that TPA-induced PKC translocation was paralleled by a marked and irreversible inhibition of intercellular coupling. These results indicate that, in contrast to the effects on transformed or dedifferentiated permanent cell lines, PKC is not involved in gating gap junctional channels between primary differentiated secretory cells of the pancreas.
Persistent Identifierhttp://hdl.handle.net/10722/132777
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChanson, Men_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorSpray, DCen_HK
dc.contributor.authorRegazzi, Ren_HK
dc.contributor.authorMeda, Pen_HK
dc.date.accessioned2011-03-28T09:28:54Z-
dc.date.available2011-03-28T09:28:54Z-
dc.date.issued1988en_HK
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1988, v. 255 n. 5, p. 24/5en_HK
dc.identifier.issn0002-9513en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132777-
dc.description.abstractSecond messengers have been implicated in the control of communication between cells of various tissues and of a number of cell lines. To assess whether protein kinase C (PKC) is involved in the regulation of gap junctions between primary differentiated cells, we studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on PKC translocation and junctional conductance of rat pancreatic exocrine cells. Our results show that although TPA induced the translocation of PKC from a 'cytosolic' to a 'microsomal' fraction within minutes, it failed to block the junctional conductance of acinar cell pairs up to 30 min after application. By contrast, analogous experiments on a liver-derived cell line (WB cells) showed that TPA-induced PKC translocation was paralleled by a marked and irreversible inhibition of intercellular coupling. These results indicate that, in contrast to the effects on transformed or dedifferentiated permanent cell lines, PKC is not involved in gating gap junctional channels between primary differentiated secretory cells of the pancreas.en_HK
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_HK
dc.titleCell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissueen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3189535-
dc.identifier.scopuseid_2-s2.0-0024256129en_HK
dc.identifier.volume255en_HK
dc.identifier.issue5en_HK
dc.identifier.spage24/5en_HK
dc.identifier.epage24/5en_HK
dc.identifier.isiWOS:A1988Q957300016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChanson, M=7004131733en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridSpray, DC=7102905269en_HK
dc.identifier.scopusauthoridRegazzi, R=7004590576en_HK
dc.identifier.scopusauthoridMeda, P=7005822187en_HK

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