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Article: Effects of n-alcohols on junctional coupling and amylase secretion of pancreatic acinar cells

TitleEffects of n-alcohols on junctional coupling and amylase secretion of pancreatic acinar cells
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1989
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 1989, v. 139 n. 1, p. 147-156 How to Cite?
AbstractWe have tested the effects of alcohols differing by their alkyl chain length on the membrane channels and amylase secretion of rat pancreatic acinar cells. In intact acini, alcohols with a chain of seven, eight, or nine carbons (C-7, C-8, and C-9) induced dye uncoupling and increased basal amylase release. These effects were readily reversible after alcohol removal. By contrast, an alcohol with a chain of 15 carbons (C-15) and several alcohols with chains of fewer than six carbons (C-2, C-4, and C-6) did not uncouple acinar cells and had no effects on amylase secretion. Neither did alkanes and oxidized derivatives of C-7 and C-8 alcohols did not affect dye coupling. Double patch-clamp experiments on pairs of acinar cells, under conditions of strong cytosolic Ca2+ and pH buffering, showed that C-7, C-8, and C-9 alcohols blocked completely and reversibly the electrical conductance of junctional channels. Furthermore, studies of single voltage-clamped acinar cells revealed that the uncoupling alcohols did not affect the resting nonjunctional membrane conductances. Thus the alcohols that did not affect acinar cell coupling did not affect amylase secretion, whereas the alcohols that caused uncoupling increased secretion. The latter effects was not mediated by changes in the conductance of nonjunctional membrane, cytosolic Ca2+, and pH and, as revealed by an immunological hemolytic plaque assay for amylase, had a time course consistent with the rapid (within 1 min) inhibition of coupling. These data provide new support for the view that the regulation of cell-to-cell communications is correlated with that of digestive enzyme secretion.
Persistent Identifierhttp://hdl.handle.net/10722/132774
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChanson men_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorBosco, Den_HK
dc.contributor.authorMeda, Pen_HK
dc.date.accessioned2011-03-28T09:28:53Z-
dc.date.available2011-03-28T09:28:53Z-
dc.date.issued1989en_HK
dc.identifier.citationJournal Of Cellular Physiology, 1989, v. 139 n. 1, p. 147-156en_HK
dc.identifier.issn0021-9541en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132774-
dc.description.abstractWe have tested the effects of alcohols differing by their alkyl chain length on the membrane channels and amylase secretion of rat pancreatic acinar cells. In intact acini, alcohols with a chain of seven, eight, or nine carbons (C-7, C-8, and C-9) induced dye uncoupling and increased basal amylase release. These effects were readily reversible after alcohol removal. By contrast, an alcohol with a chain of 15 carbons (C-15) and several alcohols with chains of fewer than six carbons (C-2, C-4, and C-6) did not uncouple acinar cells and had no effects on amylase secretion. Neither did alkanes and oxidized derivatives of C-7 and C-8 alcohols did not affect dye coupling. Double patch-clamp experiments on pairs of acinar cells, under conditions of strong cytosolic Ca2+ and pH buffering, showed that C-7, C-8, and C-9 alcohols blocked completely and reversibly the electrical conductance of junctional channels. Furthermore, studies of single voltage-clamped acinar cells revealed that the uncoupling alcohols did not affect the resting nonjunctional membrane conductances. Thus the alcohols that did not affect acinar cell coupling did not affect amylase secretion, whereas the alcohols that caused uncoupling increased secretion. The latter effects was not mediated by changes in the conductance of nonjunctional membrane, cytosolic Ca2+, and pH and, as revealed by an immunological hemolytic plaque assay for amylase, had a time course consistent with the rapid (within 1 min) inhibition of coupling. These data provide new support for the view that the regulation of cell-to-cell communications is correlated with that of digestive enzyme secretion.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_HK
dc.relation.ispartofJournal of Cellular Physiologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.titleEffects of n-alcohols on junctional coupling and amylase secretion of pancreatic acinar cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2468678-
dc.identifier.scopuseid_2-s2.0-0024557352en_HK
dc.identifier.volume139en_HK
dc.identifier.issue1en_HK
dc.identifier.spage147en_HK
dc.identifier.epage156en_HK
dc.identifier.isiWOS:A1989U346300020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChanson m=7004131733en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridBosco, D=7005328418en_HK
dc.identifier.scopusauthoridMeda, P=7005822187en_HK

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