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Article: Expression of a dominant negative inhibitor of intercellular communication in the early Xenopus embryo causes delamination and extrusion of cells

TitleExpression of a dominant negative inhibitor of intercellular communication in the early Xenopus embryo causes delamination and extrusion of cells
Authors
KeywordsConnexin
Dominant negative mutation
Gap junction
Intercellular communication
Issue Date1995
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
Citation
Development, 1995, v. 121 n. 2, p. 371-381 How to Cite?
AbstractA chimeric construct, termed 3243H7, composed of fused portions of the rat gap junction proteins connexin32 (Cx32) and connexin43 (Cx43) has been shown to have selective dominant inhibitory activity when tested in the Xenopus oocyte pair system. Co-injection of mRNA coding for 3243H7 together with mRNAs coding for Cx32 or Cx43 completely blocked the development of channel conductances, while the construct was ineffective at blocking intercellular channel assembly when coinjected with rat connexin37 (Cx37). Injection of 3243H7 into the right anterodorsal blastomere of 8-cell-stage Xenopus embryos resulted in disadhesion and delamination of the resultant clone of cells evident by embryonic stage 8; a substantial number, although not all, of the progeny of the injected cell were eliminated from the embryo by stage 12. A second construct, 3243H8, differing from 3243H7 in the relative position of the middle splice, had no dominant negative activity in the oocyte pair assay, nor any detectable effects on Xenopus development, even when injected at four-fold higher concentrations. The 3243H7-induced embryonic defects could be rescued by coinjection of Cx37 with 3243H7. A blastomere reaggregation assay was used to demonstrate that a depression of dye-transfer could be detected in 3243H7-injected cells as early as stage 7; Lucifer yellow injections into single cells also demonstrated that injection of 3243H7 resulted in a block of intercellular communication. These experiments indicate that maintenance of embryonic cell adhesion with concomitant positional information requires gap junction-mediated intercellular communication.
Persistent Identifierhttp://hdl.handle.net/10722/132753
ISSN
2015 Impact Factor: 6.059
2015 SCImago Journal Rankings: 5.239
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPaul, DLen_HK
dc.contributor.authorYu, Ken_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorGimlich, RLen_HK
dc.contributor.authorGoodenough, DAen_HK
dc.date.accessioned2011-03-28T09:28:45Z-
dc.date.available2011-03-28T09:28:45Z-
dc.date.issued1995en_HK
dc.identifier.citationDevelopment, 1995, v. 121 n. 2, p. 371-381en_HK
dc.identifier.issn0950-1991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132753-
dc.description.abstractA chimeric construct, termed 3243H7, composed of fused portions of the rat gap junction proteins connexin32 (Cx32) and connexin43 (Cx43) has been shown to have selective dominant inhibitory activity when tested in the Xenopus oocyte pair system. Co-injection of mRNA coding for 3243H7 together with mRNAs coding for Cx32 or Cx43 completely blocked the development of channel conductances, while the construct was ineffective at blocking intercellular channel assembly when coinjected with rat connexin37 (Cx37). Injection of 3243H7 into the right anterodorsal blastomere of 8-cell-stage Xenopus embryos resulted in disadhesion and delamination of the resultant clone of cells evident by embryonic stage 8; a substantial number, although not all, of the progeny of the injected cell were eliminated from the embryo by stage 12. A second construct, 3243H8, differing from 3243H7 in the relative position of the middle splice, had no dominant negative activity in the oocyte pair assay, nor any detectable effects on Xenopus development, even when injected at four-fold higher concentrations. The 3243H7-induced embryonic defects could be rescued by coinjection of Cx37 with 3243H7. A blastomere reaggregation assay was used to demonstrate that a depression of dye-transfer could be detected in 3243H7-injected cells as early as stage 7; Lucifer yellow injections into single cells also demonstrated that injection of 3243H7 resulted in a block of intercellular communication. These experiments indicate that maintenance of embryonic cell adhesion with concomitant positional information requires gap junction-mediated intercellular communication.en_HK
dc.languageengen_US
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.orgen_HK
dc.relation.ispartofDevelopmenten_HK
dc.subjectConnexinen_HK
dc.subjectDominant negative mutationen_HK
dc.subjectGap junctionen_HK
dc.subjectIntercellular communicationen_HK
dc.titleExpression of a dominant negative inhibitor of intercellular communication in the early Xenopus embryo causes delamination and extrusion of cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7768179-
dc.identifier.scopuseid_2-s2.0-0028921640en_HK
dc.identifier.volume121en_HK
dc.identifier.issue2en_HK
dc.identifier.spage371en_HK
dc.identifier.epage381en_HK
dc.identifier.isiWOS:A1995QK89600010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPaul, DL=7401667165en_HK
dc.identifier.scopusauthoridYu, K=7403385940en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridGimlich, RL=6602847860en_HK
dc.identifier.scopusauthoridGoodenough, DA=7102378382en_HK

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