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Article: High-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay

TitleHigh-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assay
Authors
KeywordsCocktail library
High-throughput screening
HIV
HTS
NNRTI
Pseudotyped lentiviral particles
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/antiviral
Citation
Antiviral Research, 2009, v. 81 n. 3, p. 239-247 How to Cite?
AbstractTwo decades after its discovery the human immunodeficiency virus (HIV) is still spreading worldwide and killing millions. There are 25 drugs formally approved for HIV currently on the market, but side effects as well as the emergence of HIV strains showing single or multiple resistances to current drug-therapy are causes for concern. Furthermore, these drugs target only 4 steps of the viral cycle, hence the urgent need for new drugs and also new targets. In order to tackle this problem, we have devised a cell-based assay using lentiviral particles to look for post-entry inhibitors of HIV-1. We report here the assay development, validation as well as confirmation of the hits using both wild-type and drug-resistant HIV-1 viruses. The screening was performed on an original library, rich in natural compounds and pure molecules from Traditional Chinese Medicine pharmacopoeia, which had never been screened for anti-HIV activity. The identified hits belong to four chemical sub-families that appear to be all non-nucleoside reverse transcriptase inhibitors (NNRTIs). Secondary tests with live viruses showed that there was good agreement with pseudotyped particles, confirming the validity of this approach for high-throughput drug screens. This assay will be a useful tool that can be easily adapted to screen for inhibitors of viral entry. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/132751
ISSN
2015 Impact Factor: 4.909
2015 SCImago Journal Rankings: 2.080
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorGao, Aen_HK
dc.contributor.authorHe, PLen_HK
dc.contributor.authorChoi, Jen_HK
dc.contributor.authorTang, Wen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorSchwartz, Oen_HK
dc.contributor.authorNaya, Hen_HK
dc.contributor.authorNan, FJen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorAltmeyer, Ren_HK
dc.contributor.authorZuo, JPen_HK
dc.date.accessioned2011-03-28T09:28:44Z-
dc.date.available2011-03-28T09:28:44Z-
dc.date.issued2009en_HK
dc.identifier.citationAntiviral Research, 2009, v. 81 n. 3, p. 239-247en_HK
dc.identifier.issn0166-3542en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132751-
dc.description.abstractTwo decades after its discovery the human immunodeficiency virus (HIV) is still spreading worldwide and killing millions. There are 25 drugs formally approved for HIV currently on the market, but side effects as well as the emergence of HIV strains showing single or multiple resistances to current drug-therapy are causes for concern. Furthermore, these drugs target only 4 steps of the viral cycle, hence the urgent need for new drugs and also new targets. In order to tackle this problem, we have devised a cell-based assay using lentiviral particles to look for post-entry inhibitors of HIV-1. We report here the assay development, validation as well as confirmation of the hits using both wild-type and drug-resistant HIV-1 viruses. The screening was performed on an original library, rich in natural compounds and pure molecules from Traditional Chinese Medicine pharmacopoeia, which had never been screened for anti-HIV activity. The identified hits belong to four chemical sub-families that appear to be all non-nucleoside reverse transcriptase inhibitors (NNRTIs). Secondary tests with live viruses showed that there was good agreement with pseudotyped particles, confirming the validity of this approach for high-throughput drug screens. This assay will be a useful tool that can be easily adapted to screen for inhibitors of viral entry. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/antiviralen_HK
dc.relation.ispartofAntiviral Researchen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Antiviral Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Antiviral Research, 2009, v. 81 n. 3, p. 239-247. DOI: 10.1016/j.antiviral.2008.12.004-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCocktail libraryen_HK
dc.subjectHigh-throughput screeningen_HK
dc.subjectHIVen_HK
dc.subjectHTSen_HK
dc.subjectNNRTIen_HK
dc.subjectPseudotyped lentiviral particlesen_HK
dc.subject.meshAnti-HIV Agents - pharmacology-
dc.subject.meshCell Line-
dc.subject.meshDrug Evaluation, Preclinical - methods-
dc.subject.meshHIV-1 - drug effects - genetics-
dc.subject.meshVirus Replication - drug effects-
dc.titleHigh-throughput screening using pseudotyped lentiviral particles: A strategy for the identification of HIV-1 inhibitors in a cell-based assayen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturepostprinten_US
dc.identifier.doi10.1016/j.antiviral.2008.12.004en_HK
dc.identifier.pmid19118579-
dc.identifier.scopuseid_2-s2.0-60249084658en_HK
dc.identifier.hkuros162412-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60249084658&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume81en_HK
dc.identifier.issue3en_HK
dc.identifier.spage239en_HK
dc.identifier.epage247en_HK
dc.identifier.isiWOS:000264278400007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridGao, A=26422900700en_HK
dc.identifier.scopusauthoridHe, PL=9245945200en_HK
dc.identifier.scopusauthoridChoi, J=37107042500en_HK
dc.identifier.scopusauthoridTang, W=53982170400en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridSchwartz, O=7006284121en_HK
dc.identifier.scopusauthoridNaya, H=6603463277en_HK
dc.identifier.scopusauthoridNan, FJ=7003880780en_HK
dc.identifier.scopusauthoridLi, J=25921542100en_HK
dc.identifier.scopusauthoridAltmeyer, R=7003677186en_HK
dc.identifier.scopusauthoridZuo, JP=7202871921en_HK

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