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Article: Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family

TitleCloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family
Authors
Keywordsγ connexin subgroup
Channels
Cloning
Gap junctions
Intercellular communication
Neurons
Oocytes
Retinal connexins
Issue Date1998
PublisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.org
Citation
Journal Of Neuroscience, 1998, v. 18 n. 19, p. 7625-7637 How to Cite?
AbstractWe have cloned cDNAs for two closely related connexins (Cx), Cx35 and Cx34.7, from a perch retinal cDNA library. Sequencing of PCR products from genomic DNA revealed that both connexins have an intron 71 bp after the translation initiation site; in Cx35, the intron is 900 bp in length, whereas in Cx34.7 it is ~20 kb. Southern blots of genomic DNA suggest that the two connexins represent independent single copy genes. In Northern blots, Cx35 and Cx34.7 transcripts were detected in retina and brain; Cx34.7 also showed a weak signal in smooth muscle (gut) RNA. Antibodies against Cx35 labeled a 30 kDa band on a Western blot of retinal membranes, and in histological sections, the pattern of antibody recognition was consistent with labeling of bipolar cells and unidentified processes in the inner plexiform and nerve fiber layers. When expressed in Xenopus oocytes, Cx35 and Cx34.7 formed homotypic gap junctions, but the junctional conductance between paired oocytes expressing Cx35 was 10-fold greater than that recorded for gap junctional channels formed by Cx34.7. The homotypic gap-junctional channels were closed in a voltage-dependent manner but with relatively weak voltage sensitivity. Heterotypic gap junctions formed by Cx35 and Cx34.7 displayed junctional conductances similar to those of Cx34.7 homotypic pairs and showed a slightly asymmetric current-voltage relationship; the side expressing Cx35 exhibited a higher sensitivity to transjunctional potentials. An analysis of the sequence and gene structure of the connexin family revealed that perch Cx35 and Cx34.7, skate Cx35, and mouse Cx36 constitute a novel γ subgroup.
Persistent Identifierhttp://hdl.handle.net/10722/132733
ISSN
2015 Impact Factor: 5.924
2015 SCImago Journal Rankings: 5.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorO'Brien, Jen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorWhite, TWen_HK
dc.contributor.authorAlUbaidi, MRen_HK
dc.contributor.authorRipps, Hen_HK
dc.date.accessioned2011-03-28T09:28:38Z-
dc.date.available2011-03-28T09:28:38Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Neuroscience, 1998, v. 18 n. 19, p. 7625-7637en_HK
dc.identifier.issn0270-6474en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132733-
dc.description.abstractWe have cloned cDNAs for two closely related connexins (Cx), Cx35 and Cx34.7, from a perch retinal cDNA library. Sequencing of PCR products from genomic DNA revealed that both connexins have an intron 71 bp after the translation initiation site; in Cx35, the intron is 900 bp in length, whereas in Cx34.7 it is ~20 kb. Southern blots of genomic DNA suggest that the two connexins represent independent single copy genes. In Northern blots, Cx35 and Cx34.7 transcripts were detected in retina and brain; Cx34.7 also showed a weak signal in smooth muscle (gut) RNA. Antibodies against Cx35 labeled a 30 kDa band on a Western blot of retinal membranes, and in histological sections, the pattern of antibody recognition was consistent with labeling of bipolar cells and unidentified processes in the inner plexiform and nerve fiber layers. When expressed in Xenopus oocytes, Cx35 and Cx34.7 formed homotypic gap junctions, but the junctional conductance between paired oocytes expressing Cx35 was 10-fold greater than that recorded for gap junctional channels formed by Cx34.7. The homotypic gap-junctional channels were closed in a voltage-dependent manner but with relatively weak voltage sensitivity. Heterotypic gap junctions formed by Cx35 and Cx34.7 displayed junctional conductances similar to those of Cx34.7 homotypic pairs and showed a slightly asymmetric current-voltage relationship; the side expressing Cx35 exhibited a higher sensitivity to transjunctional potentials. An analysis of the sequence and gene structure of the connexin family revealed that perch Cx35 and Cx34.7, skate Cx35, and mouse Cx36 constitute a novel γ subgroup.en_HK
dc.languageengen_US
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.orgen_HK
dc.relation.ispartofJournal of Neuroscienceen_HK
dc.subjectγ connexin subgroupen_HK
dc.subjectChannelsen_HK
dc.subjectCloningen_HK
dc.subjectGap junctionsen_HK
dc.subjectIntercellular communicationen_HK
dc.subjectNeuronsen_HK
dc.subjectOocytesen_HK
dc.subjectRetinal connexinsen_HK
dc.titleCloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin familyen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9742134-
dc.identifier.scopuseid_2-s2.0-0032190086en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032190086&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue19en_HK
dc.identifier.spage7625en_HK
dc.identifier.epage7637en_HK
dc.identifier.isiWOS:000076060100002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridO'Brien, J=7403406771en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridWhite, TW=35499703300en_HK
dc.identifier.scopusauthoridAlUbaidi, MR=7004717462en_HK
dc.identifier.scopusauthoridRipps, H=7005410758en_HK

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