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Article: Molecular and functional diversity of neural connexins in the retina

TitleMolecular and functional diversity of neural connexins in the retina
Authors
KeywordsCloning
Electrical synapses
Functional diversity
Gap junctions
Retina
Zebrafish connexins
Issue Date2000
PublisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.org
Citation
Journal Of Neuroscience, 2000, v. 20 n. 22, p. 8331-8343 How to Cite?
AbstractElectrical synapses (gap junctions) in neuronal circuits have become a major focus in the study of network properties such as synchronization and oscillation (Galarreta and Hestrin, 1999; Gibson et al., 1999). Despite the recent progress made in unraveling the contribution of gap junctions to network behavior, little is known about the molecular composition of the junctional constituents. By cloning gap junction proteins [connexins (Cxs)] from zebrafish retina and through functional expression, we demonstrate that the retina possesses a high degree of connexin diversity, which may account for differential functional properties of electrical synapses. Three new Cxs, designated as zebrafish Cx27.5 (zfCx27.5), zfCx44.1, and zfCx55.5, and the carp ortholog of mammalian Cx43 were cloned. By in situ hybridization and in situ RT-PCR, we demonstrate that the four fish connexin mRNAs show differential localization in the retina. Transient functional expression in paired Xenopus oocytes and in the neuroblastoma N2A cell line indicate an extreme range of electrophysiological properties of these connexins in terms of voltage dependence and unitary conductance. For instance, the new zfCx44.1 exhibited high sensitivity to voltage-induced closure with currents decaying rapidly for transjunctional potentials >10 mV, whereas zfCx55.5 channels showed an opposite voltage dependence in response to voltage steps of either polarity. Moreover, although zfCx44.1 channels showed unitary conductance as high as any previously reported for junctional channels (nearly 300 pS), zfCx55.5 and zfCx27.5 exhibited much lower unitary conductances (<60 pS).
Persistent Identifierhttp://hdl.handle.net/10722/132722
ISSN
2015 Impact Factor: 5.924
2015 SCImago Journal Rankings: 5.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDermietzel, Ren_HK
dc.contributor.authorKremer, Men_HK
dc.contributor.authorPaputsoglu, Gen_HK
dc.contributor.authorStang, Aen_HK
dc.contributor.authorSkerrett, IMen_HK
dc.contributor.authorGomes, Den_HK
dc.contributor.authorSrinivas, Men_HK
dc.contributor.authorJanssenBienhold, Uen_HK
dc.contributor.authorWeiler, Ren_HK
dc.contributor.authorNicholson, BJen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorSpray, DCen_HK
dc.date.accessioned2011-03-28T09:28:33Z-
dc.date.available2011-03-28T09:28:33Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Neuroscience, 2000, v. 20 n. 22, p. 8331-8343en_HK
dc.identifier.issn0270-6474en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132722-
dc.description.abstractElectrical synapses (gap junctions) in neuronal circuits have become a major focus in the study of network properties such as synchronization and oscillation (Galarreta and Hestrin, 1999; Gibson et al., 1999). Despite the recent progress made in unraveling the contribution of gap junctions to network behavior, little is known about the molecular composition of the junctional constituents. By cloning gap junction proteins [connexins (Cxs)] from zebrafish retina and through functional expression, we demonstrate that the retina possesses a high degree of connexin diversity, which may account for differential functional properties of electrical synapses. Three new Cxs, designated as zebrafish Cx27.5 (zfCx27.5), zfCx44.1, and zfCx55.5, and the carp ortholog of mammalian Cx43 were cloned. By in situ hybridization and in situ RT-PCR, we demonstrate that the four fish connexin mRNAs show differential localization in the retina. Transient functional expression in paired Xenopus oocytes and in the neuroblastoma N2A cell line indicate an extreme range of electrophysiological properties of these connexins in terms of voltage dependence and unitary conductance. For instance, the new zfCx44.1 exhibited high sensitivity to voltage-induced closure with currents decaying rapidly for transjunctional potentials >10 mV, whereas zfCx55.5 channels showed an opposite voltage dependence in response to voltage steps of either polarity. Moreover, although zfCx44.1 channels showed unitary conductance as high as any previously reported for junctional channels (nearly 300 pS), zfCx55.5 and zfCx27.5 exhibited much lower unitary conductances (<60 pS).en_HK
dc.languageengen_US
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.orgen_HK
dc.relation.ispartofJournal of Neuroscienceen_HK
dc.subjectCloningen_HK
dc.subjectElectrical synapsesen_HK
dc.subjectFunctional diversityen_HK
dc.subjectGap junctionsen_HK
dc.subjectRetinaen_HK
dc.subjectZebrafish connexinsen_HK
dc.titleMolecular and functional diversity of neural connexins in the retinaen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11069940-
dc.identifier.scopuseid_2-s2.0-0034668904en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034668904&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue22en_HK
dc.identifier.spage8331en_HK
dc.identifier.epage8343en_HK
dc.identifier.isiWOS:000165131500018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDermietzel, R=7005778543en_HK
dc.identifier.scopusauthoridKremer, M=7101664224en_HK
dc.identifier.scopusauthoridPaputsoglu, G=6506735759en_HK
dc.identifier.scopusauthoridStang, A=7006324389en_HK
dc.identifier.scopusauthoridSkerrett, IM=6506087208en_HK
dc.identifier.scopusauthoridGomes, D=7005538633en_HK
dc.identifier.scopusauthoridSrinivas, M=35496858800en_HK
dc.identifier.scopusauthoridJanssenBienhold, U=6603040192en_HK
dc.identifier.scopusauthoridWeiler, R=7005537169en_HK
dc.identifier.scopusauthoridNicholson, BJ=7103204557en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridSpray, DC=7102905269en_HK

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