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Article: Cell coupling and Cx43 expression in embryonic mouse neural progenitor cells

TitleCell coupling and Cx43 expression in embryonic mouse neural progenitor cells
Authors
KeywordsAstrocyte
CNS
Connexin
Gap junction
Neuron
Oligodendrocyte
Issue Date2002
PublisherThe Company of Biologists Ltd
Citation
Journal Of Cell Science, 2002, v. 115 n. 16, p. 3241-3251 How to Cite?
AbstractEmbryonic neural progenitors isolated from the mouse striatal germinal zone grow in vitro as floating cell aggregates called neurospheres, which, upon adhesion, can be induced to differentiate into the three main cell types of the central nervous system (CNS), that is, astrocytes, neurons and oligodendrocytes. To study the possible role of connexins and junctional communication during differentiation of neural progenitors, we assessed cell-to-cell communication by microinjecting Lucifer Yellow into neurospheres at various times after adhesion. Cells located in neurospheres were strongly coupled, regardless of the differentiation time. Microinjections performed on the cell layers formed by differentiated cells migrating out of the neurosphere established that only astrocytes were coupled. These observations suggest the existence of at least three distinct communication compartments: coupled proliferating cells located in the sphere, uncoupled cells undergoing neuronal or oligodendrocytic differentiation and coupled differentiating astrocytes. A blockade of junctional communication by 18-β-glycyrrhetinic acid (βGA) reduced, in a concentration-dependent manner, the viability of undifferentiated neural progenitor cells. This effect appeared to be specific, inasmuch as it was reversible and that cell survival was not affected in the presence of the inactive analog glycyrrhyzic acid. Addition of βGA to adherent neurospheres also decreased cell density and altered the morphology of differentiated cells. Cx43 was strongly expressed in either undifferentiated or differentiated neurospheres, where it was found both within the sphere and in astrocytes, the two cell populations that were dye coupled. Western blot analysis further showed that Cx43 phosphorylation was strongly increased in adherent neurospheres, suggesting a post-translational regulation during differentiation. These results point to a major role of cell-to-cell communication and Cx43 during the differentiation of neural progenitor cells in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/132711
ISSN
2015 Impact Factor: 4.706
2015 SCImago Journal Rankings: 3.501
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDuval, Nen_HK
dc.contributor.authorGomès, Den_HK
dc.contributor.authorCalaora, Ven_HK
dc.contributor.authorCalabrese, Aen_HK
dc.contributor.authorMeda, Pen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.date.accessioned2011-03-28T09:28:27Z-
dc.date.available2011-03-28T09:28:27Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Cell Science, 2002, v. 115 n. 16, p. 3241-3251en_HK
dc.identifier.issn0021-9533en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132711-
dc.description.abstractEmbryonic neural progenitors isolated from the mouse striatal germinal zone grow in vitro as floating cell aggregates called neurospheres, which, upon adhesion, can be induced to differentiate into the three main cell types of the central nervous system (CNS), that is, astrocytes, neurons and oligodendrocytes. To study the possible role of connexins and junctional communication during differentiation of neural progenitors, we assessed cell-to-cell communication by microinjecting Lucifer Yellow into neurospheres at various times after adhesion. Cells located in neurospheres were strongly coupled, regardless of the differentiation time. Microinjections performed on the cell layers formed by differentiated cells migrating out of the neurosphere established that only astrocytes were coupled. These observations suggest the existence of at least three distinct communication compartments: coupled proliferating cells located in the sphere, uncoupled cells undergoing neuronal or oligodendrocytic differentiation and coupled differentiating astrocytes. A blockade of junctional communication by 18-β-glycyrrhetinic acid (βGA) reduced, in a concentration-dependent manner, the viability of undifferentiated neural progenitor cells. This effect appeared to be specific, inasmuch as it was reversible and that cell survival was not affected in the presence of the inactive analog glycyrrhyzic acid. Addition of βGA to adherent neurospheres also decreased cell density and altered the morphology of differentiated cells. Cx43 was strongly expressed in either undifferentiated or differentiated neurospheres, where it was found both within the sphere and in astrocytes, the two cell populations that were dye coupled. Western blot analysis further showed that Cx43 phosphorylation was strongly increased in adherent neurospheres, suggesting a post-translational regulation during differentiation. These results point to a major role of cell-to-cell communication and Cx43 during the differentiation of neural progenitor cells in vitro.en_HK
dc.languageengen_US
dc.publisherThe Company of Biologists Ltden_US
dc.relation.ispartofJournal of Cell Scienceen_HK
dc.subjectAstrocyteen_HK
dc.subjectCNSen_HK
dc.subjectConnexinen_HK
dc.subjectGap junctionen_HK
dc.subjectNeuronen_HK
dc.subjectOligodendrocyteen_HK
dc.titleCell coupling and Cx43 expression in embryonic mouse neural progenitor cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12140256-
dc.identifier.scopuseid_2-s2.0-0037101947en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037101947&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue16en_HK
dc.identifier.spage3241en_HK
dc.identifier.epage3251en_HK
dc.identifier.isiWOS:000177825800003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDuval, N=7003504203en_HK
dc.identifier.scopusauthoridGomès, D=7005538633en_HK
dc.identifier.scopusauthoridCalaora, V=7801665223en_HK
dc.identifier.scopusauthoridCalabrese, A=7006480705en_HK
dc.identifier.scopusauthoridMeda, P=7005822187en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK

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