File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Virtual cloning, functional expression, and gating analysis of human connexin31.9

TitleVirtual cloning, functional expression, and gating analysis of human connexin31.9
Authors
KeywordsChannel
Expression
Gap junction
Gene family
Issue Date2002
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 2002, v. 283 n. 3 52-3, p. C960-C970 How to Cite?
AbstractWe have identified a novel gap junction gene by searching the human genome sequence database that encodes a protein designated as connexin31.9 (Cx31.9). Cx31.9 was most homologous to human Cx32.4 and did not cluster with either the purported α- or β-connexin subfamilies. Expression of Cx31.9 was detected by RT-PCR in human mRNA from several tissues including cerebral cortex, heart, liver, lung, kidney, spleen, and testis. A partial Cx31.9 sequence was also represented in the human Expressed Sequence Tag database. Cx31.9 formed intercellular channels in both paired Xenopus oocytes and transfected neuroblastoma N2A cells that were distinguished by an apparent low unitary conductance (12-15 pS) and a remarkable insensitivity to transjunctional voltage. In contrast, Cx31.9 channels were gated by cytoplasmic acidification or exposure to halothane like other connexins. Cx31.9 was able to form heterotypic channels with the highly voltage-sensitive Xenopus Cx38 (XenCx38), which provides an opportunity to study gating in heterotypic channels formed by hemichannels (connexons) composed of connexins with widely divergent properties. Thus Cx31.9 is a novel human connexin that forms channels with unique functional properties.
Persistent Identifierhttp://hdl.handle.net/10722/132710
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWhite, TWen_HK
dc.contributor.authorSrinivas, Men_HK
dc.contributor.authorRipps, Hen_HK
dc.contributor.authorTrovatoSalinaro, Aen_HK
dc.contributor.authorCondorelli, DFen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.date.accessioned2011-03-28T09:28:27Z-
dc.date.available2011-03-28T09:28:27Z-
dc.date.issued2002en_HK
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 2002, v. 283 n. 3 52-3, p. C960-C970en_HK
dc.identifier.issn0363-6143en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132710-
dc.description.abstractWe have identified a novel gap junction gene by searching the human genome sequence database that encodes a protein designated as connexin31.9 (Cx31.9). Cx31.9 was most homologous to human Cx32.4 and did not cluster with either the purported α- or β-connexin subfamilies. Expression of Cx31.9 was detected by RT-PCR in human mRNA from several tissues including cerebral cortex, heart, liver, lung, kidney, spleen, and testis. A partial Cx31.9 sequence was also represented in the human Expressed Sequence Tag database. Cx31.9 formed intercellular channels in both paired Xenopus oocytes and transfected neuroblastoma N2A cells that were distinguished by an apparent low unitary conductance (12-15 pS) and a remarkable insensitivity to transjunctional voltage. In contrast, Cx31.9 channels were gated by cytoplasmic acidification or exposure to halothane like other connexins. Cx31.9 was able to form heterotypic channels with the highly voltage-sensitive Xenopus Cx38 (XenCx38), which provides an opportunity to study gating in heterotypic channels formed by hemichannels (connexons) composed of connexins with widely divergent properties. Thus Cx31.9 is a novel human connexin that forms channels with unique functional properties.en_HK
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_HK
dc.subjectChannelen_HK
dc.subjectExpressionen_HK
dc.subjectGap junctionen_HK
dc.subjectGene familyen_HK
dc.titleVirtual cloning, functional expression, and gating analysis of human connexin31.9en_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12176752-
dc.identifier.scopuseid_2-s2.0-0036720805en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036720805&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume283en_HK
dc.identifier.issue3 52-3en_HK
dc.identifier.spageC960en_HK
dc.identifier.epageC970en_HK
dc.identifier.isiWOS:000177573500030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWhite, TW=35499703300en_HK
dc.identifier.scopusauthoridSrinivas, M=35496858800en_HK
dc.identifier.scopusauthoridRipps, H=7005410758en_HK
dc.identifier.scopusauthoridTrovatoSalinaro, A=6603265494en_HK
dc.identifier.scopusauthoridCondorelli, DF=7005993790en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats