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Article: Connexin-dependent inter-cellular communication increases invasion and dissemination of Shigella in epithelial cells

TitleConnexin-dependent inter-cellular communication increases invasion and dissemination of Shigella in epithelial cells
Authors
KeywordsSpecies Index: Animalia
Bacteria (Microorganisms)
Shigella
Shigella Flexneri
Issue Date2003
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiology
Citation
Nature Cell Biology, 2003, v. 5 n. 8, p. 720-726 How to Cite?
AbstractShigella flexneri, the causative agent of bacillar dystentery, invades the colonic mucosa where it elicits an intense inflammatory reaction responsible for destruction of the epithelium1,2. During cell invasion, contact with host cells activates the type-III secretion of the Shigella IpaB and IpaC proteins3,4. IpaB and IpaC are inserted into host cell plasma membranes and trigger initial signals that result in actin polymerization, while allowing cytosolic access of other bacterial effectors that further reorganize the cytoskeleton5,6. After internalization, Shigella moves intracellularly and forms protrusions that infect neighbouring cells, promoting bacterial dissemination across the epithelium2,6. Here, we show that during cell invasion, Shigella induces transient peaks in intracellular calcium concentration that are dependent on a functional type-III secretory apparatus. In addition, Shigella invasion induces the opening of Connexin 26 (Cx26) hemichannels in an actin- and phospholipase-C-dependent manner, allowing release of ATP into the medium. The released ATP, in turn, increases bacterial invasion and spreading, as well as calcium signalling induced by Shigella. These results provide evidence that pathogen-induced opening of connexin channels promotes signalling events that favour bacterial invasion and dissemination.
Persistent Identifierhttp://hdl.handle.net/10722/132705
ISSN
2015 Impact Factor: 18.699
2015 SCImago Journal Rankings: 14.131
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorVan Nhieu, GTen_HK
dc.contributor.authorClair, Cen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorMesnil, Men_HK
dc.contributor.authorSansonetti, Pen_HK
dc.contributor.authorCombettes, Len_HK
dc.date.accessioned2011-03-28T09:28:24Z-
dc.date.available2011-03-28T09:28:24Z-
dc.date.issued2003en_HK
dc.identifier.citationNature Cell Biology, 2003, v. 5 n. 8, p. 720-726en_HK
dc.identifier.issn1465-7392en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132705-
dc.description.abstractShigella flexneri, the causative agent of bacillar dystentery, invades the colonic mucosa where it elicits an intense inflammatory reaction responsible for destruction of the epithelium1,2. During cell invasion, contact with host cells activates the type-III secretion of the Shigella IpaB and IpaC proteins3,4. IpaB and IpaC are inserted into host cell plasma membranes and trigger initial signals that result in actin polymerization, while allowing cytosolic access of other bacterial effectors that further reorganize the cytoskeleton5,6. After internalization, Shigella moves intracellularly and forms protrusions that infect neighbouring cells, promoting bacterial dissemination across the epithelium2,6. Here, we show that during cell invasion, Shigella induces transient peaks in intracellular calcium concentration that are dependent on a functional type-III secretory apparatus. In addition, Shigella invasion induces the opening of Connexin 26 (Cx26) hemichannels in an actin- and phospholipase-C-dependent manner, allowing release of ATP into the medium. The released ATP, in turn, increases bacterial invasion and spreading, as well as calcium signalling induced by Shigella. These results provide evidence that pathogen-induced opening of connexin channels promotes signalling events that favour bacterial invasion and dissemination.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiologyen_HK
dc.relation.ispartofNature Cell Biologyen_HK
dc.subjectSpecies Index: Animaliaen_US
dc.subjectBacteria (Microorganisms)en_US
dc.subjectShigellaen_US
dc.subjectShigella Flexnerien_US
dc.titleConnexin-dependent inter-cellular communication increases invasion and dissemination of Shigella in epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/ncb1021en_HK
dc.identifier.pmid12844145-
dc.identifier.scopuseid_2-s2.0-0043198046en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0043198046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue8en_HK
dc.identifier.spage720en_HK
dc.identifier.epage726en_HK
dc.identifier.isiWOS:000184687300013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.f10001015346-
dc.identifier.scopusauthoridVan Nhieu, GT=6701455661en_HK
dc.identifier.scopusauthoridClair, C=6604048074en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridMesnil, M=7003333102en_HK
dc.identifier.scopusauthoridSansonetti, P=7103154727en_HK
dc.identifier.scopusauthoridCombettes, L=7004200927en_HK

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