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Article: Molecular cloning and functional expression of zfCx52.6: A novel connexin with hemichannel-forming properties expressed in horizontal cells of the zebrafish retina

TitleMolecular cloning and functional expression of zfCx52.6: A novel connexin with hemichannel-forming properties expressed in horizontal cells of the zebrafish retina
Authors
KeywordsSpecies Index: Animalia
Danio Rerio
Vertebrata
Issue Date2004
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2004, v. 279 n. 4, p. 2913-2921 How to Cite?
AbstractGap junction-mediated electrical coupling contributes to synchronous oscillatory activities of neurons, and considerable progress has been made in defining the molecular composition of gap junction channels. In particular, cloning and functional expression of gap junction proteins (connexins (Cx)) from zebrafish retina have shown that this part of the brain possesses a high degree of connexin diversity that may account for differential functional properties of electrical synapses. Here, we report the cloning and functional characterization of a new connexin, designated zebrafish Cx52.6 (zfCx52.6). This connexin shows little similarity to known connexins from fish and higher vertebrates. By combining in situ hybridization with Laser Capture Microdissection and RT-PCR, we found that this novel fish connexin is expressed in horizontal cells in the inner nuclear layer of the retina. Functional expression of zfCx52.6 in neuroblastoma cells and Xenopus oocytes led to functional gap junctional channels and, in single oocytes, induced large non-junctional membrane currents indicative of the formation of hemichannels, which were inhibited in reversible fashion by raising extracellular Ca 2+ concentrations.
Persistent Identifierhttp://hdl.handle.net/10722/132703
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZoidl, Gen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorWeickert, Sen_HK
dc.contributor.authorKremer, Men_HK
dc.contributor.authorZoidl, Cen_HK
dc.contributor.authorMitropoulou, Gen_HK
dc.contributor.authorSrinivas, Men_HK
dc.contributor.authorSpray, DCen_HK
dc.contributor.authorDermietzel, Ren_HK
dc.date.accessioned2011-03-28T09:28:23Z-
dc.date.available2011-03-28T09:28:23Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2004, v. 279 n. 4, p. 2913-2921en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132703-
dc.description.abstractGap junction-mediated electrical coupling contributes to synchronous oscillatory activities of neurons, and considerable progress has been made in defining the molecular composition of gap junction channels. In particular, cloning and functional expression of gap junction proteins (connexins (Cx)) from zebrafish retina have shown that this part of the brain possesses a high degree of connexin diversity that may account for differential functional properties of electrical synapses. Here, we report the cloning and functional characterization of a new connexin, designated zebrafish Cx52.6 (zfCx52.6). This connexin shows little similarity to known connexins from fish and higher vertebrates. By combining in situ hybridization with Laser Capture Microdissection and RT-PCR, we found that this novel fish connexin is expressed in horizontal cells in the inner nuclear layer of the retina. Functional expression of zfCx52.6 in neuroblastoma cells and Xenopus oocytes led to functional gap junctional channels and, in single oocytes, induced large non-junctional membrane currents indicative of the formation of hemichannels, which were inhibited in reversible fashion by raising extracellular Ca 2+ concentrations.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.subjectSpecies Index: Animaliaen_US
dc.subjectDanio Rerioen_US
dc.subjectVertebrataen_US
dc.titleMolecular cloning and functional expression of zfCx52.6: A novel connexin with hemichannel-forming properties expressed in horizontal cells of the zebrafish retinaen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.M304850200en_HK
dc.identifier.pmid14583621-
dc.identifier.scopuseid_2-s2.0-1642273622en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642273622&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume279en_HK
dc.identifier.issue4en_HK
dc.identifier.spage2913en_HK
dc.identifier.epage2921en_HK
dc.identifier.isiWOS:000188211300071-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZoidl, G=6602432080en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridWeickert, S=6505902956en_HK
dc.identifier.scopusauthoridKremer, M=7101664224en_HK
dc.identifier.scopusauthoridZoidl, C=6603600306en_HK
dc.identifier.scopusauthoridMitropoulou, G=8772857700en_HK
dc.identifier.scopusauthoridSrinivas, M=35496858800en_HK
dc.identifier.scopusauthoridSpray, DC=7102905269en_HK
dc.identifier.scopusauthoridDermietzel, R=7005778543en_HK

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