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Article: Pharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes

TitlePharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes
Authors
KeywordsCarbenoxolone
Connexin
Electrical synapses
Flufenamic acid
Gap junction
Gating
Issue Date2005
PublisherBlackwell Publishing Ltd
Citation
Journal Of Neurochemistry, 2005, v. 92 n. 5, p. 1033-1043 How to Cite?
AbstractSeveral new findings have emphasized the role of neuron-specific gap junction proteins (connexins) and electrical synapses in processing sensory information and in synchronizing the activity of neuronal networks. We have recently shown that pannexins constitute an additional family of proteins that can form gap junction channels in a heterologous expression system and are also widely expressed in distinct neuronal populations in the brain, where they may represent a novel class of electrical synapses. In this study, we have exploited the hemichannel-forming properties of pannexins to investigate their sensitivity to well-known connexin blockers. By combining biochemical and electrophysiological approaches, we report here further evidence for the interaction of pannexin1 (Px1) with Px2 and demonstrate that the pharmacological sensitivity of heteromeric Px1/Px2 is similar to that of homomeric Px1 channels. In contrast to most connexins, both Px1 and Px1/Px2 hemichannels were not gated by external Ca2+. In addition, they exhibited a remarkable sensitivity to blockade by carbenoxolone (with an IC50 of ∼5 μM), whereas flufenamic acid exerted only a modest inhibitory effect. The opposite was true in the case of connexin46 (Cx46), thus indicating that gap junction blockers are able to selectively modulate pannexin and connexin channels. © 2005 International Society for Neurochemistry.
Persistent Identifierhttp://hdl.handle.net/10722/132698
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorBarbe, MTen_HK
dc.contributor.authorJakob, NJen_HK
dc.contributor.authorMonyer, Hen_HK
dc.date.accessioned2011-03-28T09:28:20Z-
dc.date.available2011-03-28T09:28:20Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Neurochemistry, 2005, v. 92 n. 5, p. 1033-1043en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132698-
dc.description.abstractSeveral new findings have emphasized the role of neuron-specific gap junction proteins (connexins) and electrical synapses in processing sensory information and in synchronizing the activity of neuronal networks. We have recently shown that pannexins constitute an additional family of proteins that can form gap junction channels in a heterologous expression system and are also widely expressed in distinct neuronal populations in the brain, where they may represent a novel class of electrical synapses. In this study, we have exploited the hemichannel-forming properties of pannexins to investigate their sensitivity to well-known connexin blockers. By combining biochemical and electrophysiological approaches, we report here further evidence for the interaction of pannexin1 (Px1) with Px2 and demonstrate that the pharmacological sensitivity of heteromeric Px1/Px2 is similar to that of homomeric Px1 channels. In contrast to most connexins, both Px1 and Px1/Px2 hemichannels were not gated by external Ca2+. In addition, they exhibited a remarkable sensitivity to blockade by carbenoxolone (with an IC50 of ∼5 μM), whereas flufenamic acid exerted only a modest inhibitory effect. The opposite was true in the case of connexin46 (Cx46), thus indicating that gap junction blockers are able to selectively modulate pannexin and connexin channels. © 2005 International Society for Neurochemistry.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltden_US
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.subjectCarbenoxoloneen_HK
dc.subjectConnexinen_HK
dc.subjectElectrical synapsesen_HK
dc.subjectFlufenamic aciden_HK
dc.subjectGap junctionen_HK
dc.subjectGatingen_HK
dc.titlePharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytesen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1471-4159.2004.02947.xen_HK
dc.identifier.pmid15715654-
dc.identifier.scopuseid_2-s2.0-14844334146en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14844334146&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1033en_HK
dc.identifier.epage1043en_HK
dc.identifier.isiWOS:000226964900004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridBarbe, MT=7005131360en_HK
dc.identifier.scopusauthoridJakob, NJ=6507388839en_HK
dc.identifier.scopusauthoridMonyer, H=35600652500en_HK
dc.identifier.citeulike93978-

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