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Article: Blockage of cell-to-cell communication within pancreatic acini is associated with increased basal release of amylase

TitleBlockage of cell-to-cell communication within pancreatic acini is associated with increased basal release of amylase
Authors
Issue Date1986
PublisherRockefeller University Press. The Journal's web site is located at http://www.jcb.org
Citation
Journal Of Cell Biology, 1986, v. 103 n. 2, p. 475-483 How to Cite?
AbstractTo assess whether junctional coupling is involved in the secretory activity of pancreatic acinar cells, dispersed rat acini were incubated for 30 min in the presence of either heptanol (3.5 mM) or octanol (1.0 mM). Exposure to either alkanol caused a marked uncoupling of the acini cells which, in control acini, were extensively coupled. Uncoupling was associated with an increased basal release of amylase that was at least twice that of controls. By contrast, carbamylcholine (10-5 M)-induced maximal amylase secretion, cytosolic pH, and free Ca2+, as well as the structure of gap junctions joining the acini cells, were unaffected. Both uncoupling and the alteration of basal secretion were already observed after only 5 min of exposure to heptanol, they both persisted throughout the 30-min exposure of the alkanols, and were reversible after removal of either heptanol or octanol. Since neither of the two uncouplers appeared to alter unspecifically the secretory machinery and the nonjuctional membrane of acinar cells, the data are consistent with the view that junctional coupling participates in the control of the basal secretion of acinar cells.
Persistent Identifierhttp://hdl.handle.net/10722/132678
ISSN
2015 Impact Factor: 8.717
2015 SCImago Journal Rankings: 7.923
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMeda, Pen_HK
dc.contributor.authorBruzzone, Ren_HK
dc.contributor.authorKnodel, Sen_HK
dc.contributor.authorOrci, Len_HK
dc.date.accessioned2011-03-28T09:28:10Z-
dc.date.available2011-03-28T09:28:10Z-
dc.date.issued1986en_HK
dc.identifier.citationJournal Of Cell Biology, 1986, v. 103 n. 2, p. 475-483en_HK
dc.identifier.issn0021-9525en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132678-
dc.description.abstractTo assess whether junctional coupling is involved in the secretory activity of pancreatic acinar cells, dispersed rat acini were incubated for 30 min in the presence of either heptanol (3.5 mM) or octanol (1.0 mM). Exposure to either alkanol caused a marked uncoupling of the acini cells which, in control acini, were extensively coupled. Uncoupling was associated with an increased basal release of amylase that was at least twice that of controls. By contrast, carbamylcholine (10-5 M)-induced maximal amylase secretion, cytosolic pH, and free Ca2+, as well as the structure of gap junctions joining the acini cells, were unaffected. Both uncoupling and the alteration of basal secretion were already observed after only 5 min of exposure to heptanol, they both persisted throughout the 30-min exposure of the alkanols, and were reversible after removal of either heptanol or octanol. Since neither of the two uncouplers appeared to alter unspecifically the secretory machinery and the nonjuctional membrane of acinar cells, the data are consistent with the view that junctional coupling participates in the control of the basal secretion of acinar cells.en_HK
dc.languageengen_US
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jcb.orgen_HK
dc.relation.ispartofJournal of Cell Biologyen_HK
dc.titleBlockage of cell-to-cell communication within pancreatic acini is associated with increased basal release of amylaseen_HK
dc.typeArticleen_HK
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hken_HK
dc.identifier.authorityBruzzone, R=rp01442en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1083/jcb.103.2.475-
dc.identifier.pmid2426281-
dc.identifier.scopuseid_2-s2.0-0022526848en_HK
dc.identifier.volume103en_HK
dc.identifier.issue2en_HK
dc.identifier.spage475en_HK
dc.identifier.epage483en_HK
dc.identifier.isiWOS:A1986D465300017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMeda, P=7005822187en_HK
dc.identifier.scopusauthoridBruzzone, R=7006793327en_HK
dc.identifier.scopusauthoridKnodel, S=6507826100en_HK
dc.identifier.scopusauthoridOrci, L=35468097500en_HK

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