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Article: A Ca2+-linked increase in coupled cAMP synthesis and hydrolysis is an early event in cholinergic and β-adrenergic stimulation of parotid secretion

TitleA Ca2+-linked increase in coupled cAMP synthesis and hydrolysis is an early event in cholinergic and β-adrenergic stimulation of parotid secretion
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1988
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1988, v. 85 n. 21, p. 7867-7871 How to Cite?
AbstractThe dynamics and compartmental characteristics of cAMP metabolism were examined by 18O labeling of cellular adenine nucleotide α phosphoryls in rat parotid gland stimulated to secrete with β-adrenergic and cholinergic agents. The secretory response occurred in association with a rapidly increased rate of cAMP hydrolysis apparently coordinated with an equivalent increase in the rate of cAMP synthesis, since the cellular concentration of cAMP remained unchanged. The magnitude of this metabolic response was equivalent to the metabolism of 10-75 times the cellular content of cAMP within the first minute of stimulation. This increased metabolic rate occurred only during the early (1-3 min) period of stimulation, in what appeared to be an exclusive cellular compartment distinguished by a unique distribution of 18O among adenine nucleotide α phosphoryls. This 18O distribution contrasted with that produced by forskolin, which increased cellular cAMP concentration and elicited only a delayed response missing the early secretory component. The early acceleration of cAMP metabolism appeared linked to a stimulus-induced increase in intracellular Ca2+ concentration, since the Ca2+ ionophore ionomycin produced the same metabolic response in association with secretion. These observations suggest that cAMP metabolism is involved in stimulus-secretion coupling by a Ca2+-linked mechanism different from that in which cAMP plays the role of a second messenger.
Persistent Identifierhttp://hdl.handle.net/10722/132591
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDeeg, MAen_HK
dc.contributor.authorGraeff, RMen_HK
dc.contributor.authorWalseth, TFen_HK
dc.contributor.authorGoldberg, NDen_HK
dc.date.accessioned2011-03-28T09:26:34Z-
dc.date.available2011-03-28T09:26:34Z-
dc.date.issued1988en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1988, v. 85 n. 21, p. 7867-7871en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132591-
dc.description.abstractThe dynamics and compartmental characteristics of cAMP metabolism were examined by 18O labeling of cellular adenine nucleotide α phosphoryls in rat parotid gland stimulated to secrete with β-adrenergic and cholinergic agents. The secretory response occurred in association with a rapidly increased rate of cAMP hydrolysis apparently coordinated with an equivalent increase in the rate of cAMP synthesis, since the cellular concentration of cAMP remained unchanged. The magnitude of this metabolic response was equivalent to the metabolism of 10-75 times the cellular content of cAMP within the first minute of stimulation. This increased metabolic rate occurred only during the early (1-3 min) period of stimulation, in what appeared to be an exclusive cellular compartment distinguished by a unique distribution of 18O among adenine nucleotide α phosphoryls. This 18O distribution contrasted with that produced by forskolin, which increased cellular cAMP concentration and elicited only a delayed response missing the early secretory component. The early acceleration of cAMP metabolism appeared linked to a stimulus-induced increase in intracellular Ca2+ concentration, since the Ca2+ ionophore ionomycin produced the same metabolic response in association with secretion. These observations suggest that cAMP metabolism is involved in stimulus-secretion coupling by a Ca2+-linked mechanism different from that in which cAMP plays the role of a second messenger.en_HK
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.titleA Ca2+-linked increase in coupled cAMP synthesis and hydrolysis is an early event in cholinergic and β-adrenergic stimulation of parotid secretionen_HK
dc.typeArticleen_US
dc.identifier.emailGraeff, RM: graeffr@hku.hken_HK
dc.identifier.authorityGraeff, RM=rp01464en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2460856-
dc.identifier.scopuseid_2-s2.0-0024110529en_HK
dc.identifier.volume85en_HK
dc.identifier.issue21en_HK
dc.identifier.spage7867en_HK
dc.identifier.epage7871en_HK
dc.identifier.isiWOS:A1988Q834100009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDeeg, MA=7004751463en_HK
dc.identifier.scopusauthoridGraeff, RM=7003614053en_HK
dc.identifier.scopusauthoridWalseth, TF=7005424273en_HK
dc.identifier.scopusauthoridGoldberg, ND=23085828700en_HK

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