File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1006/bbrc.1994.2725
- Scopus: eid_2-s2.0-0027937693
- PMID: 7999103
- WOS: WOS:A1994PW78500106
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: GDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cells
Title | GDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cells |
---|---|
Authors | |
Keywords | Chemicals And Cas Registry Numbers |
Issue Date | 1994 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 1994, v. 205 n. 1, p. 722-727 How to Cite? |
Abstract | Retinoic acid (RA) treatment of HL-60 cells induces surface expression of CD38. This lymphocytic antigen is also a novel bifunctional enzyme catalyzing the synthesis and hydrolysis of cyclic ADP-ribose (cADPR), a Ca2+ mobilizing metabolite of NAD+. The synthetic activity of CD38 is very difficult to detect because of the concurrent hydrolytic activity. In this study, a Ca2+ release assay capable of detecting submicromolar concentrations of cADPR was used to demonstrate the induction of ADP-ribosyl cyclase activity in HL-60 cells by RA. Concommitantly, cADPR hydrolase activity was also increased. The results were further substantiated by using a newly developed assay for GDP-ribosyl cyclase activity. This assay uses NGD+ as substrate instead of NAD+. The resulting fluorescent product, cyclic GDP-ribose, is resistant to hydrolysis and accumulates, making it a highly sensitive and convenient assay for CD38-like enzymes. |
Persistent Identifier | http://hdl.handle.net/10722/132584 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Graeff, RM | en_HK |
dc.contributor.author | Mehta, K | en_HK |
dc.contributor.author | Lee, HC | en_HK |
dc.date.accessioned | 2011-03-28T09:26:32Z | - |
dc.date.available | 2011-03-28T09:26:32Z | - |
dc.date.issued | 1994 | en_HK |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 1994, v. 205 n. 1, p. 722-727 | en_HK |
dc.identifier.issn | 0006-291X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/132584 | - |
dc.description.abstract | Retinoic acid (RA) treatment of HL-60 cells induces surface expression of CD38. This lymphocytic antigen is also a novel bifunctional enzyme catalyzing the synthesis and hydrolysis of cyclic ADP-ribose (cADPR), a Ca2+ mobilizing metabolite of NAD+. The synthetic activity of CD38 is very difficult to detect because of the concurrent hydrolytic activity. In this study, a Ca2+ release assay capable of detecting submicromolar concentrations of cADPR was used to demonstrate the induction of ADP-ribosyl cyclase activity in HL-60 cells by RA. Concommitantly, cADPR hydrolase activity was also increased. The results were further substantiated by using a newly developed assay for GDP-ribosyl cyclase activity. This assay uses NGD+ as substrate instead of NAD+. The resulting fluorescent product, cyclic GDP-ribose, is resistant to hydrolysis and accumulates, making it a highly sensitive and convenient assay for CD38-like enzymes. | en_HK |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_HK |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_US |
dc.title | GDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Graeff, RM: graeffr@hku.hk | en_HK |
dc.identifier.email | Lee, HC: leehc@hku.hk | en_HK |
dc.identifier.authority | Graeff, RM=rp01464 | en_HK |
dc.identifier.authority | Lee, HC=rp00545 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1006/bbrc.1994.2725 | en_HK |
dc.identifier.pmid | 7999103 | - |
dc.identifier.scopus | eid_2-s2.0-0027937693 | en_HK |
dc.identifier.volume | 205 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 722 | en_HK |
dc.identifier.epage | 727 | en_HK |
dc.identifier.isi | WOS:A1994PW78500106 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Graeff, RM=7003614053 | en_HK |
dc.identifier.scopusauthorid | Mehta, K=7201939452 | en_HK |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_HK |
dc.identifier.issnl | 0006-291X | - |