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Article: GDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cells

TitleGDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cells
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1994, v. 205 n. 1, p. 722-727 How to Cite?
AbstractRetinoic acid (RA) treatment of HL-60 cells induces surface expression of CD38. This lymphocytic antigen is also a novel bifunctional enzyme catalyzing the synthesis and hydrolysis of cyclic ADP-ribose (cADPR), a Ca2+ mobilizing metabolite of NAD+. The synthetic activity of CD38 is very difficult to detect because of the concurrent hydrolytic activity. In this study, a Ca2+ release assay capable of detecting submicromolar concentrations of cADPR was used to demonstrate the induction of ADP-ribosyl cyclase activity in HL-60 cells by RA. Concommitantly, cADPR hydrolase activity was also increased. The results were further substantiated by using a newly developed assay for GDP-ribosyl cyclase activity. This assay uses NGD+ as substrate instead of NAD+. The resulting fluorescent product, cyclic GDP-ribose, is resistant to hydrolysis and accumulates, making it a highly sensitive and convenient assay for CD38-like enzymes.
Persistent Identifierhttp://hdl.handle.net/10722/132584
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGraeff, RMen_HK
dc.contributor.authorMehta, Ken_HK
dc.contributor.authorLee, HCen_HK
dc.date.accessioned2011-03-28T09:26:32Z-
dc.date.available2011-03-28T09:26:32Z-
dc.date.issued1994en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 1994, v. 205 n. 1, p. 722-727en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/132584-
dc.description.abstractRetinoic acid (RA) treatment of HL-60 cells induces surface expression of CD38. This lymphocytic antigen is also a novel bifunctional enzyme catalyzing the synthesis and hydrolysis of cyclic ADP-ribose (cADPR), a Ca2+ mobilizing metabolite of NAD+. The synthetic activity of CD38 is very difficult to detect because of the concurrent hydrolytic activity. In this study, a Ca2+ release assay capable of detecting submicromolar concentrations of cADPR was used to demonstrate the induction of ADP-ribosyl cyclase activity in HL-60 cells by RA. Concommitantly, cADPR hydrolase activity was also increased. The results were further substantiated by using a newly developed assay for GDP-ribosyl cyclase activity. This assay uses NGD+ as substrate instead of NAD+. The resulting fluorescent product, cyclic GDP-ribose, is resistant to hydrolysis and accumulates, making it a highly sensitive and convenient assay for CD38-like enzymes.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.titleGDP-ribosyl cyclase activity as a measure of CD38 induction by retinoic acid in HL-60 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailGraeff, RM: graeffr@hku.hken_HK
dc.identifier.emailLee, HC: leehc@hku.hken_HK
dc.identifier.authorityGraeff, RM=rp01464en_HK
dc.identifier.authorityLee, HC=rp00545en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/bbrc.1994.2725en_HK
dc.identifier.pmid7999103-
dc.identifier.scopuseid_2-s2.0-0027937693en_HK
dc.identifier.volume205en_HK
dc.identifier.issue1en_HK
dc.identifier.spage722en_HK
dc.identifier.epage727en_HK
dc.identifier.isiWOS:A1994PW78500106-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGraeff, RM=7003614053en_HK
dc.identifier.scopusauthoridMehta, K=7201939452en_HK
dc.identifier.scopusauthoridLee, HC=26642959100en_HK

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