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Article: Activation and inactivation of Ca 2+ release by NAADP +

TitleActivation and inactivation of Ca 2+ release by NAADP +
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1996
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1996, v. 271 n. 15, p. 8513-8516 How to Cite?
AbstractNicotinic acid adenine dinucleotide phosphate (NAADP +) is a recently identified metabolite of NADP + that is as potent as inositol trisphosphate (IP 3) and cyclic ADP-ribose (cADPR) in mobilizing intracellular Ca 2+ in sea urchin eggs and microsomes (Clapper, D. L., Walseth, T. F., Dargie, P. J., and Lee, H. C. (1987) J. Biol. Chem. 262, 9561-9568; Lee, H. C., and Aarhus, R. (1995) J. Biol. Chem. 270, 2152-2157). The mechanism of Ca 2+ release activated by NAADP + and the Ca 2+ stores it acts on are different from those of IP 3 and cADPR. In this study we show that photolyzing caged NAADP + in intact sea urchin eggs elicits long term Ca 2+ oscillations. On the other hand, uncaging threshold amounts of NAADP + produces desensitization. In microsomes, this self-inactivation mechanism exhibits concentration and time dependance. Binding studies show that the NAADP + recaptor is distinct from that of cADPR, and at subthreshold concentrations, NAADP + can fully inactirate subsequent binding to the receptor in a time- dependent manner. Thus, the NAADP +-sensitive Ca 2+ release process has novel regulatory characteristics, which are distinguishable from Ca 2+ release mediated by either IP 3 or cADPR. This battery of release mechanisms may provide the necessary versatility for cells to respond to diverse signals that lead to Ca 2+ mobilization.
Persistent Identifierhttp://hdl.handle.net/10722/132577
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
References

 

DC FieldValueLanguage
dc.contributor.authorAarhus, Ren_HK
dc.contributor.authorDickey, DMen_HK
dc.contributor.authorGraeff, RMen_HK
dc.contributor.authorGee, KRen_HK
dc.contributor.authorWalseth, TFen_HK
dc.contributor.authorHon Cheung Leeen_HK
dc.date.accessioned2011-03-28T09:26:28Z-
dc.date.available2011-03-28T09:26:28Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal Of Biological Chemistry, 1996, v. 271 n. 15, p. 8513-8516en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132577-
dc.description.abstractNicotinic acid adenine dinucleotide phosphate (NAADP +) is a recently identified metabolite of NADP + that is as potent as inositol trisphosphate (IP 3) and cyclic ADP-ribose (cADPR) in mobilizing intracellular Ca 2+ in sea urchin eggs and microsomes (Clapper, D. L., Walseth, T. F., Dargie, P. J., and Lee, H. C. (1987) J. Biol. Chem. 262, 9561-9568; Lee, H. C., and Aarhus, R. (1995) J. Biol. Chem. 270, 2152-2157). The mechanism of Ca 2+ release activated by NAADP + and the Ca 2+ stores it acts on are different from those of IP 3 and cADPR. In this study we show that photolyzing caged NAADP + in intact sea urchin eggs elicits long term Ca 2+ oscillations. On the other hand, uncaging threshold amounts of NAADP + produces desensitization. In microsomes, this self-inactivation mechanism exhibits concentration and time dependance. Binding studies show that the NAADP + recaptor is distinct from that of cADPR, and at subthreshold concentrations, NAADP + can fully inactirate subsequent binding to the receptor in a time- dependent manner. Thus, the NAADP +-sensitive Ca 2+ release process has novel regulatory characteristics, which are distinguishable from Ca 2+ release mediated by either IP 3 or cADPR. This battery of release mechanisms may provide the necessary versatility for cells to respond to diverse signals that lead to Ca 2+ mobilization.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.titleActivation and inactivation of Ca 2+ release by NAADP +en_HK
dc.typeArticleen_HK
dc.identifier.emailGraeff, RM: graeffr@hku.hken_HK
dc.identifier.emailHon Cheung Lee: leehc@hku.hken_HK
dc.identifier.authorityGraeff, RM=rp01464en_HK
dc.identifier.authorityHon Cheung Lee=rp00545en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.271.15.8513en_HK
dc.identifier.pmid8621471-
dc.identifier.scopuseid_2-s2.0-0029664620en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029664620&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume271en_HK
dc.identifier.issue15en_HK
dc.identifier.spage8513en_HK
dc.identifier.epage8516en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridAarhus, R=6701339421en_HK
dc.identifier.scopusauthoridDickey, DM=55292530300en_HK
dc.identifier.scopusauthoridGraeff, RM=7003614053en_HK
dc.identifier.scopusauthoridGee, KR=7101946977en_HK
dc.identifier.scopusauthoridWalseth, TF=7005424273en_HK
dc.identifier.scopusauthoridHon Cheung Lee=26642959100en_HK

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