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Article: High throughput fluorescence-based assays for cyclic ADP-ribose, NAADP, and their metabolic enzymes

TitleHigh throughput fluorescence-based assays for cyclic ADP-ribose, NAADP, and their metabolic enzymes
Authors
KeywordsADP-ribosyl cyclase
Calcium messengers
Calcium signaling
Calcium stores
CD38
Cyclic ADP-ribose
NAADP
Pyridine nucleotides
Issue Date2003
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/cchts
Citation
Combinatorial Chemistry And High Throughput Screening, 2003, v. 6 n. 4, p. 367-379 How to Cite?
AbstractCyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are two novel Ca2+ messengers derived respectively from NAD and NADP. Since their discovery in sea urchin eggs, both have now been shown to serve messenger functions in a wide range of cells from plant to human. In this article, a series of fluorimetric assays for cADPR, NAADP and their metabolic enzymes is compiled. The enzyme assay makes use of an analog of NAD, nicotinamide guanine dinucleotide, which is non-fluorescent but is cyclized by the enzymes to a fluorescent analog of cADPR, cyclic GDP-ribose. Other NAD utilizing enzymes are not capable of catalyzing the cyclization and thus produce no interference. The fluorimetric assays for cADPR and NAADP make use of coupled-enzyme amplification and can readily detect nanomolar concentrations of either messenger. All the assays described can be performed in multi-well format, allowing ready automation and use in high throughput screening. An added advantage of these assays is that all the required reagents are commercially available, facilitating general adoption of the techniques by all those who are interested in the physiology and enzymology of the novel Ca2+ signaling pathways mediated by cADPR and NAADP.
Persistent Identifierhttp://hdl.handle.net/10722/132562
ISSN
2015 Impact Factor: 1.041
2015 SCImago Journal Rankings: 0.357
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGraeff, RMen_HK
dc.contributor.authorLee, HCen_HK
dc.date.accessioned2011-03-28T09:26:19Z-
dc.date.available2011-03-28T09:26:19Z-
dc.date.issued2003en_HK
dc.identifier.citationCombinatorial Chemistry And High Throughput Screening, 2003, v. 6 n. 4, p. 367-379en_HK
dc.identifier.issn1386-2073en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132562-
dc.description.abstractCyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are two novel Ca2+ messengers derived respectively from NAD and NADP. Since their discovery in sea urchin eggs, both have now been shown to serve messenger functions in a wide range of cells from plant to human. In this article, a series of fluorimetric assays for cADPR, NAADP and their metabolic enzymes is compiled. The enzyme assay makes use of an analog of NAD, nicotinamide guanine dinucleotide, which is non-fluorescent but is cyclized by the enzymes to a fluorescent analog of cADPR, cyclic GDP-ribose. Other NAD utilizing enzymes are not capable of catalyzing the cyclization and thus produce no interference. The fluorimetric assays for cADPR and NAADP make use of coupled-enzyme amplification and can readily detect nanomolar concentrations of either messenger. All the assays described can be performed in multi-well format, allowing ready automation and use in high throughput screening. An added advantage of these assays is that all the required reagents are commercially available, facilitating general adoption of the techniques by all those who are interested in the physiology and enzymology of the novel Ca2+ signaling pathways mediated by cADPR and NAADP.en_HK
dc.languageengen_US
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/cchtsen_HK
dc.relation.ispartofCombinatorial Chemistry and High Throughput Screeningen_HK
dc.subjectADP-ribosyl cyclaseen_HK
dc.subjectCalcium messengersen_HK
dc.subjectCalcium signalingen_HK
dc.subjectCalcium storesen_HK
dc.subjectCD38en_HK
dc.subjectCyclic ADP-riboseen_HK
dc.subjectNAADPen_HK
dc.subjectPyridine nucleotidesen_HK
dc.titleHigh throughput fluorescence-based assays for cyclic ADP-ribose, NAADP, and their metabolic enzymesen_HK
dc.typeArticleen_HK
dc.identifier.emailGraeff, RM: graeffr@hku.hken_HK
dc.identifier.emailLee, HC: leehc@hku.hken_HK
dc.identifier.authorityGraeff, RM=rp01464en_HK
dc.identifier.authorityLee, HC=rp00545en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12769681-
dc.identifier.scopuseid_2-s2.0-0038284834en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038284834&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue4en_HK
dc.identifier.spage367en_HK
dc.identifier.epage379en_HK
dc.identifier.isiWOS:000183019400013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridGraeff, RM=7003614053en_HK
dc.identifier.scopusauthoridLee, HC=26642959100en_HK

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