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Article: Nuclear CD38 in retinoic acid-induced HL-60 cells

TitleNuclear CD38 in retinoic acid-induced HL-60 cells
Authors
KeywordsADP ribosylation
CD38
cGDP-ribose
NAD + glycohydrolase
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr
Citation
Experimental Cell Research, 2005, v. 303 n. 1, p. 14-21 How to Cite?
AbstractThe cell surface antigen, CD38, is a 45-kDa transmembrane protein which is predominantly expressed on hematopoietic cells during differentiation. As a bifunctional ectoenzyme, it catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD + and hydrolysis of either NAD + or cADPR to ADP-ribose. All-trans-retinoic acid (RA) is a potent and specific inducer of CD38 in myeloid cells. In this report, we demonstrate that the nuclei of RA-treated human HL-60 myeloblastic cells reveal enzymatic activities inherent to CD38. Thus, GDP-ribosyl cyclase and NAD + glycohydrolase activities in the nuclear fraction increased very significantly in response to incubation with RA. With Western blotting, we detected in the nuclear protein fraction from RA-treated cells a ∼43-kDa protein band which was reactive with the CD38-specific monoclonal antibody OKT10. The expression of CD38 in HL-60 nuclei was also shown with FACScan analysis. RA treatment gave rise to an increase in in vitro ADP ribosylation of the ∼43-kDa nuclear protein. Moreover, nuclei isolated from RA-treated HL-60 cells revealed calcium release in response to cADPR, whereas a similar response was not observed in control nuclei. These results suggest that CD38 is expressed in HL-60 cell nuclei during RA-induced differentiation. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/132559
ISSN
2015 Impact Factor: 3.378
2015 SCImago Journal Rankings: 1.900
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYalcintepe, Len_HK
dc.contributor.authorAlbeniz, Ien_HK
dc.contributor.authorAdinCinar, Sen_HK
dc.contributor.authorTiryaki, Den_HK
dc.contributor.authorBermek, Een_HK
dc.contributor.authorGraeff, RMen_HK
dc.contributor.authorLee, HCen_HK
dc.date.accessioned2011-03-28T09:26:17Z-
dc.date.available2011-03-28T09:26:17Z-
dc.date.issued2005en_HK
dc.identifier.citationExperimental Cell Research, 2005, v. 303 n. 1, p. 14-21en_HK
dc.identifier.issn0014-4827en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132559-
dc.description.abstractThe cell surface antigen, CD38, is a 45-kDa transmembrane protein which is predominantly expressed on hematopoietic cells during differentiation. As a bifunctional ectoenzyme, it catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD + and hydrolysis of either NAD + or cADPR to ADP-ribose. All-trans-retinoic acid (RA) is a potent and specific inducer of CD38 in myeloid cells. In this report, we demonstrate that the nuclei of RA-treated human HL-60 myeloblastic cells reveal enzymatic activities inherent to CD38. Thus, GDP-ribosyl cyclase and NAD + glycohydrolase activities in the nuclear fraction increased very significantly in response to incubation with RA. With Western blotting, we detected in the nuclear protein fraction from RA-treated cells a ∼43-kDa protein band which was reactive with the CD38-specific monoclonal antibody OKT10. The expression of CD38 in HL-60 nuclei was also shown with FACScan analysis. RA treatment gave rise to an increase in in vitro ADP ribosylation of the ∼43-kDa nuclear protein. Moreover, nuclei isolated from RA-treated HL-60 cells revealed calcium release in response to cADPR, whereas a similar response was not observed in control nuclei. These results suggest that CD38 is expressed in HL-60 cell nuclei during RA-induced differentiation. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcren_HK
dc.relation.ispartofExperimental Cell Researchen_HK
dc.subjectADP ribosylationen_HK
dc.subjectCD38en_HK
dc.subjectcGDP-riboseen_HK
dc.subjectNAD + glycohydrolaseen_HK
dc.titleNuclear CD38 in retinoic acid-induced HL-60 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailGraeff, RM: graeffr@hku.hken_HK
dc.identifier.emailLee, HC: leehc@hku.hken_HK
dc.identifier.authorityGraeff, RM=rp01464en_HK
dc.identifier.authorityLee, HC=rp00545en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.yexcr.2004.09.010en_HK
dc.identifier.pmid15572023-
dc.identifier.scopuseid_2-s2.0-9644263908en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9644263908&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume303en_HK
dc.identifier.issue1en_HK
dc.identifier.spage14en_HK
dc.identifier.epage21en_HK
dc.identifier.isiWOS:000226111600002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYalcintepe, L=7801526828en_HK
dc.identifier.scopusauthoridAlbeniz, I=6507006678en_HK
dc.identifier.scopusauthoridAdinCinar, S=6507712671en_HK
dc.identifier.scopusauthoridTiryaki, D=6506686737en_HK
dc.identifier.scopusauthoridBermek, E=7003504827en_HK
dc.identifier.scopusauthoridGraeff, RM=7003614053en_HK
dc.identifier.scopusauthoridLee, HC=26642959100en_HK

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