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Article: Cell-Cell Interaction Underlies Formation Of Fluid In The Male Reproductive Tract Of The Rat

TitleCell-Cell Interaction Underlies Formation Of Fluid In The Male Reproductive Tract Of The Rat
Authors
KeywordsBasal cells
Calcium
Chloride secretion
Epididymis
Transient receptor potential protein
Issue Date2005
PublisherRockefeller University Press. The Journal's web site is located at www.jgp.org/
Citation
Journal Of General Physiology, 2005, v. 125 n. 5, p. 443-454 How to Cite?
AbstractThe Epithelia Lining The Epididymides Of Many Species Consists Of Several Cell Types. We Have Provided Evidence That The Basal Cells Are Essential To The Integrated Functions Of The Epithelium. Basal Cells, But Not Principal Cells, And Other Cells In The Epididymis Express Trpc3 And Cox-1. We Have Isolated Basal Cells From Intact Rat Epididymis Using Antibody-Coated Dynabeads And Subjected Them To Whole-Cell Patch-Clamp Measurement Of Nonselective Cation Channel Activity, A Feature Of Trpc3 Protein, And Fluo-3 Fluorescence Measurement Of Intracellular Ca2+ Concentration. The Results Show That A Nonselective Cation Current Blockable By La3+ (0.1 Mm), Gd3+ (0.1 Mm), Or Skf96365 (20 Μm) Could Be Activated By Lysylbradykinin (200 Nm). In Cells Loaded With Fluo-3, Addition Of Lysylbradykinin (100 Nm) Caused A Sustained Increase Of Intracellular Ca 2+. This Effect Was Blocked By Gd3+ (0.1 Mm) Or Skf96365 (20 Μm) And Was Not Observed In Fluo-3-Loaded Principal Cells. Stimulation Of Basal Cell/Principal Cell Cocultures With Lysylbradykinin (200 Nm) Evoked In Principal Cells A Current With Cftr-Cl- Channel Characteristics. Isolated Principal Cells In The Absence Of Basal Cells Did Not Respond To Lysylbradykinin But Responded To Pge2 (100 Nm) With Activation Of A Cftr-Like Current. Basal Cells, But Not Principal Cells, Released Prostaglandin E2 When Stimulated With Lysylbradykinin (100 Nm). The Release Was Blocked By Skf96365 (20 Μm) And Bapta-Am (0.05 Or 0.1 Mm). Confluent Cell Monolayers Harvested From A Mixture Of Disaggregated Principal Cells And Basal Cells Responded To Lysylbradykinin (100 Nm) And Pge2 (500 Nm) With An Increase In Electrogenic Anion Secretion. The Former Response Was Dependent On Prostaglandin Synthesis As Piroxicam Blocked The Response. However, Cell Cultures Obtained From Principal Cells Alone Responded To Pge2 But Not To Bradykinin. These Results Support The Notion That Basal Cells Regulate Principal Cells Through A Ca2+ And Cox Signaling Pathway.
Persistent Identifierhttp://hdl.handle.net/10722/132541
ISSN
2015 Impact Factor: 4.511
2015 SCImago Journal Rankings: 3.061
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, KHen_US
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorLeung, MCTen_US
dc.contributor.authorShum, WWCen_US
dc.contributor.authorZhou, WLen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2011-03-28T09:26:07Z-
dc.date.available2011-03-28T09:26:07Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of General Physiology, 2005, v. 125 n. 5, p. 443-454en_US
dc.identifier.issn0022-1295en_US
dc.identifier.urihttp://hdl.handle.net/10722/132541-
dc.description.abstractThe Epithelia Lining The Epididymides Of Many Species Consists Of Several Cell Types. We Have Provided Evidence That The Basal Cells Are Essential To The Integrated Functions Of The Epithelium. Basal Cells, But Not Principal Cells, And Other Cells In The Epididymis Express Trpc3 And Cox-1. We Have Isolated Basal Cells From Intact Rat Epididymis Using Antibody-Coated Dynabeads And Subjected Them To Whole-Cell Patch-Clamp Measurement Of Nonselective Cation Channel Activity, A Feature Of Trpc3 Protein, And Fluo-3 Fluorescence Measurement Of Intracellular Ca2+ Concentration. The Results Show That A Nonselective Cation Current Blockable By La3+ (0.1 Mm), Gd3+ (0.1 Mm), Or Skf96365 (20 Μm) Could Be Activated By Lysylbradykinin (200 Nm). In Cells Loaded With Fluo-3, Addition Of Lysylbradykinin (100 Nm) Caused A Sustained Increase Of Intracellular Ca 2+. This Effect Was Blocked By Gd3+ (0.1 Mm) Or Skf96365 (20 Μm) And Was Not Observed In Fluo-3-Loaded Principal Cells. Stimulation Of Basal Cell/Principal Cell Cocultures With Lysylbradykinin (200 Nm) Evoked In Principal Cells A Current With Cftr-Cl- Channel Characteristics. Isolated Principal Cells In The Absence Of Basal Cells Did Not Respond To Lysylbradykinin But Responded To Pge2 (100 Nm) With Activation Of A Cftr-Like Current. Basal Cells, But Not Principal Cells, Released Prostaglandin E2 When Stimulated With Lysylbradykinin (100 Nm). The Release Was Blocked By Skf96365 (20 Μm) And Bapta-Am (0.05 Or 0.1 Mm). Confluent Cell Monolayers Harvested From A Mixture Of Disaggregated Principal Cells And Basal Cells Responded To Lysylbradykinin (100 Nm) And Pge2 (500 Nm) With An Increase In Electrogenic Anion Secretion. The Former Response Was Dependent On Prostaglandin Synthesis As Piroxicam Blocked The Response. However, Cell Cultures Obtained From Principal Cells Alone Responded To Pge2 But Not To Bradykinin. These Results Support The Notion That Basal Cells Regulate Principal Cells Through A Ca2+ And Cox Signaling Pathway.en_US
dc.languageengen_US
dc.publisherRockefeller University Press. The Journal's web site is located at www.jgp.org/en_US
dc.relation.ispartofJournal Of General Physiologyen_US
dc.rightsJournal of General Physiology. Copyright © Rockefeller University Press.-
dc.subjectBasal cellsen_US
dc.subjectCalcium-
dc.subjectChloride secretion-
dc.subjectEpididymis-
dc.subjectTransient receptor potential protein-
dc.titleCell-Cell Interaction Underlies Formation Of Fluid In The Male Reproductive Tract Of The Raten_US
dc.typeArticleen_US
dc.identifier.emailCheung, King Ho :kingho.cheung@hku.hken_US
dc.identifier.authorityCheung, King Ho =rp01463en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1085/jgp.200409205en_US
dc.identifier.pmid15851503-
dc.identifier.pmcidPMC2217504-
dc.identifier.scopuseid_2-s2.0-18544364336en_US
dc.identifier.hkuros103547-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18544364336&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume125en_US
dc.identifier.issue5en_US
dc.identifier.spage443en_US
dc.identifier.epage454en_US
dc.identifier.isiWOS:000228654000002-

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