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- Publisher Website: 10.1002/1521-4141(200205)32:5<1223::AID-IMMU1223>3.0.CO;2-5
- Scopus: eid_2-s2.0-0036000926
- PMID: 11981809
- WOS: WOS:000175695700003
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Article: Calcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cells
Title | Calcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cells |
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Authors | |
Keywords | Chemicals And Cas Registry Numbers |
Issue Date | 2002 |
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de |
Citation | European Journal Of Immunology, 2002, v. 32 n. 5, p. 1223-1229 How to Cite? |
Abstract | Calcineurin has been demonstrated as one of the key enzymes in TCR-mediated signaling cascades that lead to the transcription of a variety of cytokines including IL-2. In this study, we addressed the role of calcineurin in lymphocyte development and peripheral T cell responses using the lymphocytic choriomeningitis virus glycoprotein peptide p33-specific, TCR (P14)-transgenic T cells that were deficient in calcineurin subunit A α-isoform (CNAα-/-). Fetal thymic organ culture of P14/CNAα-/- lobes showed no defect in positive or negative selection of thymocytes. In addition, peptide-induced peripheral T cell deletion was also normal in CNAα-deficient T cells. In terms of mature T cell function, a reduction in proliferation, and IL-2 and IFN-γ production was observed upon stimulation of P14/CNAα-/- T cells with the antigenic peptide. Impaired NF-AT nuclear localization was also observed. These results suggest that CNAα is important for mature T cell function, but has a limited role in thymocyte development. |
Persistent Identifier | http://hdl.handle.net/10722/132501 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.627 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, VSF | en_HK |
dc.contributor.author | Wong, C | en_HK |
dc.contributor.author | Ohashi, PS | en_HK |
dc.date.accessioned | 2011-03-28T09:25:28Z | - |
dc.date.available | 2011-03-28T09:25:28Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | European Journal Of Immunology, 2002, v. 32 n. 5, p. 1223-1229 | en_HK |
dc.identifier.issn | 0014-2980 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/132501 | - |
dc.description.abstract | Calcineurin has been demonstrated as one of the key enzymes in TCR-mediated signaling cascades that lead to the transcription of a variety of cytokines including IL-2. In this study, we addressed the role of calcineurin in lymphocyte development and peripheral T cell responses using the lymphocytic choriomeningitis virus glycoprotein peptide p33-specific, TCR (P14)-transgenic T cells that were deficient in calcineurin subunit A α-isoform (CNAα-/-). Fetal thymic organ culture of P14/CNAα-/- lobes showed no defect in positive or negative selection of thymocytes. In addition, peptide-induced peripheral T cell deletion was also normal in CNAα-deficient T cells. In terms of mature T cell function, a reduction in proliferation, and IL-2 and IFN-γ production was observed upon stimulation of P14/CNAα-/- T cells with the antigenic peptide. Impaired NF-AT nuclear localization was also observed. These results suggest that CNAα is important for mature T cell function, but has a limited role in thymocyte development. | en_HK |
dc.language | eng | en_US |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de | en_HK |
dc.relation.ispartof | European Journal of Immunology | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_US |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Antigens, Viral - immunology | en_HK |
dc.subject.mesh | CD8-Positive T-Lymphocytes - immunology - metabolism | en_HK |
dc.subject.mesh | Calcineurin - deficiency - genetics - metabolism | en_HK |
dc.subject.mesh | Cell Differentiation | en_HK |
dc.subject.mesh | Cell Division | en_HK |
dc.subject.mesh | Clonal Deletion | en_HK |
dc.subject.mesh | DNA-Binding Proteins - metabolism | en_HK |
dc.subject.mesh | Glycoproteins - immunology | en_HK |
dc.subject.mesh | Interferon-gamma - biosynthesis | en_HK |
dc.subject.mesh | Interleukin-2 - biosynthesis | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Mice, Knockout | en_HK |
dc.subject.mesh | Mice, Transgenic | en_HK |
dc.subject.mesh | NFATC Transcription Factors | en_HK |
dc.subject.mesh | Nuclear Proteins | en_HK |
dc.subject.mesh | Peptide Fragments - immunology | en_HK |
dc.subject.mesh | Receptors, Antigen, T-Cell - metabolism | en_HK |
dc.subject.mesh | Signal Transduction | en_HK |
dc.subject.mesh | T-Lymphocytes - cytology - enzymology - immunology | en_HK |
dc.subject.mesh | Transcription Factors - metabolism | en_HK |
dc.subject.mesh | Viral Proteins - immunology | en_HK |
dc.title | Calcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chan, VSF:sfvchan@hku.hk | en_HK |
dc.identifier.authority | Chan, VSF=rp01459 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/1521-4141(200205)32:5<1223::AID-IMMU1223>3.0.CO;2-5 | en_HK |
dc.identifier.pmid | 11981809 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0036000926 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036000926&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 32 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 1223 | en_HK |
dc.identifier.epage | 1229 | en_HK |
dc.identifier.isi | WOS:000175695700003 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Chan, VSF=35200370000 | en_HK |
dc.identifier.scopusauthorid | Wong, C=36862857800 | en_HK |
dc.identifier.scopusauthorid | Ohashi, PS=7006493798 | en_HK |
dc.identifier.issnl | 0014-2980 | - |