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Article: Calcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cells

TitleCalcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cells
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2002
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 2002, v. 32 n. 5, p. 1223-1229 How to Cite?
AbstractCalcineurin has been demonstrated as one of the key enzymes in TCR-mediated signaling cascades that lead to the transcription of a variety of cytokines including IL-2. In this study, we addressed the role of calcineurin in lymphocyte development and peripheral T cell responses using the lymphocytic choriomeningitis virus glycoprotein peptide p33-specific, TCR (P14)-transgenic T cells that were deficient in calcineurin subunit A α-isoform (CNAα-/-). Fetal thymic organ culture of P14/CNAα-/- lobes showed no defect in positive or negative selection of thymocytes. In addition, peptide-induced peripheral T cell deletion was also normal in CNAα-deficient T cells. In terms of mature T cell function, a reduction in proliferation, and IL-2 and IFN-γ production was observed upon stimulation of P14/CNAα-/- T cells with the antigenic peptide. Impaired NF-AT nuclear localization was also observed. These results suggest that CNAα is important for mature T cell function, but has a limited role in thymocyte development.
Persistent Identifierhttp://hdl.handle.net/10722/132501
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, VSFen_HK
dc.contributor.authorWong, Cen_HK
dc.contributor.authorOhashi, PSen_HK
dc.date.accessioned2011-03-28T09:25:28Z-
dc.date.available2011-03-28T09:25:28Z-
dc.date.issued2002en_HK
dc.identifier.citationEuropean Journal Of Immunology, 2002, v. 32 n. 5, p. 1223-1229en_HK
dc.identifier.issn0014-2980en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132501-
dc.description.abstractCalcineurin has been demonstrated as one of the key enzymes in TCR-mediated signaling cascades that lead to the transcription of a variety of cytokines including IL-2. In this study, we addressed the role of calcineurin in lymphocyte development and peripheral T cell responses using the lymphocytic choriomeningitis virus glycoprotein peptide p33-specific, TCR (P14)-transgenic T cells that were deficient in calcineurin subunit A α-isoform (CNAα-/-). Fetal thymic organ culture of P14/CNAα-/- lobes showed no defect in positive or negative selection of thymocytes. In addition, peptide-induced peripheral T cell deletion was also normal in CNAα-deficient T cells. In terms of mature T cell function, a reduction in proliferation, and IL-2 and IFN-γ production was observed upon stimulation of P14/CNAα-/- T cells with the antigenic peptide. Impaired NF-AT nuclear localization was also observed. These results suggest that CNAα is important for mature T cell function, but has a limited role in thymocyte development.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_HK
dc.relation.ispartofEuropean Journal of Immunologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_US
dc.subject.meshAnimalsen_HK
dc.subject.meshAntigens, Viral - immunologyen_HK
dc.subject.meshCD8-Positive T-Lymphocytes - immunology - metabolismen_HK
dc.subject.meshCalcineurin - deficiency - genetics - metabolismen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshClonal Deletionen_HK
dc.subject.meshDNA-Binding Proteins - metabolismen_HK
dc.subject.meshGlycoproteins - immunologyen_HK
dc.subject.meshInterferon-gamma - biosynthesisen_HK
dc.subject.meshInterleukin-2 - biosynthesisen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshNFATC Transcription Factorsen_HK
dc.subject.meshNuclear Proteinsen_HK
dc.subject.meshPeptide Fragments - immunologyen_HK
dc.subject.meshReceptors, Antigen, T-Cell - metabolismen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshT-Lymphocytes - cytology - enzymology - immunologyen_HK
dc.subject.meshTranscription Factors - metabolismen_HK
dc.subject.meshViral Proteins - immunologyen_HK
dc.titleCalcineurin Aα plays an exclusive role in TCR signaling in mature but not in immature T cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, VSF:sfvchan@hku.hken_HK
dc.identifier.authorityChan, VSF=rp01459en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/1521-4141(200205)32:5<1223::AID-IMMU1223>3.0.CO;2-5en_HK
dc.identifier.pmid11981809en_HK
dc.identifier.scopuseid_2-s2.0-0036000926en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036000926&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1223en_HK
dc.identifier.epage1229en_HK
dc.identifier.isiWOS:000175695700003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChan, VSF=35200370000en_HK
dc.identifier.scopusauthoridWong, C=36862857800en_HK
dc.identifier.scopusauthoridOhashi, PS=7006493798en_HK

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