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Article: Conjugation of latent membrane protein (LMP)-2 epitope to gold nanoparticles as highly immunogenic multiple antigenic peptides for induction of Epstein-Barr virus-specific cytotoxic T-lymphocyte responses in vitro

TitleConjugation of latent membrane protein (LMP)-2 epitope to gold nanoparticles as highly immunogenic multiple antigenic peptides for induction of Epstein-Barr virus-specific cytotoxic T-lymphocyte responses in vitro
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/bc
Citation
Bioconjugate Chemistry, 2009, v. 20 n. 1, p. 24-31 How to Cite?
AbstractNasopharyngeal carcinoma is a neoplasm with a high incidence in Southeast Asia, and it is strongly associated with Epstein-Barr virus (EBV) activation involving the expression of a weakly immunogenic protein, namely, latent membrane protein (LMP)-2. Previous immunological studies already identified the human leukocyte antigen (HLA)-All restricted peptide epitope (SSCSSCPLSK) in the LMP-2 antigen. In this work, we prepared gold nanoparticle (AuNP)-peptide conjugate 1 by treating the nanoparticles with the Af-cysteinated LMP-2 epitope. The AuNP-peptide conjugates have been characterized by TEM (15-24 nm in diameter) and UV-vis spectroscopy (surface plasmon resonance absorption band at λmax = 520 nm). In the presence of a CALNN capping peptide, the AuNP-peptide conjugates are stable in solution without aggregation at room temperature for at least 48 h. By ELIspot studies, AuNP-peptide conjugate 1 was found to elicit a significantly stronger INF-γ response [number of spot forming cells (SPC) = 727 ± 198] from peripheral blood mononuclear cells of healthy HLA-A11 donors when compared to that induced by the unconjugated LMP-2 peptides (SFC = 73 ± 28). Further studies showed that dendritic cells treated with conjugate 1 can effect CD8+ T-cell activation leading to epitope-specific cytotoxic T lymphocyte killing responses in vitro. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/132491
ISSN
2014 Impact Factor: 4.513
2014 SCImago Journal Rankings: 1.604
ISI Accession Number ID
Funding AgencyGrant Number
Innovation and Technology Commission (HKSAR)ITS/211/01
University of Hong Kong
UGC20600433
NSFC/RGCN_HKU 721/04
RGCCERGHKU 7444/03M
Areas of Excellence SchemeAoE/P-10/01
Funding Information:

We acknowledge the financial support of the Innovation and Technology Fund (ITS/211/01) administered by the Innovation and Technology Commission (HKSAR), The University of Hong Kong (University Development Fund and UGC matching grant No. 20600433), NSFC/RGC (N_HKU 721/04), RGCCERG (HKU 7444/03M), and the Areas of Excellence Scheme (AoE/P-10/01) established under the University Grants Council, HKSAR.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorCheung, WHen_HK
dc.contributor.authorChan, VSFen_HK
dc.contributor.authorPang, HWen_HK
dc.contributor.authorWong, MKen_HK
dc.contributor.authorGuo, ZHen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorLin, CLen_HK
dc.contributor.authorYu, WYen_HK
dc.date.accessioned2011-03-28T09:25:21Z-
dc.date.available2011-03-28T09:25:21Z-
dc.date.issued2009en_HK
dc.identifier.citationBioconjugate Chemistry, 2009, v. 20 n. 1, p. 24-31en_HK
dc.identifier.issn1043-1802en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132491-
dc.description.abstractNasopharyngeal carcinoma is a neoplasm with a high incidence in Southeast Asia, and it is strongly associated with Epstein-Barr virus (EBV) activation involving the expression of a weakly immunogenic protein, namely, latent membrane protein (LMP)-2. Previous immunological studies already identified the human leukocyte antigen (HLA)-All restricted peptide epitope (SSCSSCPLSK) in the LMP-2 antigen. In this work, we prepared gold nanoparticle (AuNP)-peptide conjugate 1 by treating the nanoparticles with the Af-cysteinated LMP-2 epitope. The AuNP-peptide conjugates have been characterized by TEM (15-24 nm in diameter) and UV-vis spectroscopy (surface plasmon resonance absorption band at λmax = 520 nm). In the presence of a CALNN capping peptide, the AuNP-peptide conjugates are stable in solution without aggregation at room temperature for at least 48 h. By ELIspot studies, AuNP-peptide conjugate 1 was found to elicit a significantly stronger INF-γ response [number of spot forming cells (SPC) = 727 ± 198] from peripheral blood mononuclear cells of healthy HLA-A11 donors when compared to that induced by the unconjugated LMP-2 peptides (SFC = 73 ± 28). Further studies showed that dendritic cells treated with conjugate 1 can effect CD8+ T-cell activation leading to epitope-specific cytotoxic T lymphocyte killing responses in vitro. © 2009 American Chemical Society.en_HK
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/bcen_HK
dc.relation.ispartofBioconjugate Chemistryen_HK
dc.subject.meshAntigens, Viral - immunologyen_HK
dc.subject.meshCD8-Positive T-Lymphocytes - immunologyen_HK
dc.subject.meshCytotoxicity, Immunologicen_HK
dc.subject.meshGolden_HK
dc.subject.meshHerpesvirus 4, Human - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLymphocyte Activation - immunologyen_HK
dc.subject.meshMetal Nanoparticles - chemistryen_HK
dc.subject.meshT-Lymphocytes, Cytotoxic - immunologyen_HK
dc.subject.meshViral Matrix Proteins - chemistry - immunologyen_HK
dc.titleConjugation of latent membrane protein (LMP)-2 epitope to gold nanoparticles as highly immunogenic multiple antigenic peptides for induction of Epstein-Barr virus-specific cytotoxic T-lymphocyte responses in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, WH: sunnycwh@hku.hken_HK
dc.identifier.emailChan, VSF: sfvchan@hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.authorityCheung, WH=rp00246en_HK
dc.identifier.authorityChan, VSF=rp01459en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/bc800167qen_HK
dc.identifier.pmid19102689en_HK
dc.identifier.scopuseid_2-s2.0-61849144830en_HK
dc.identifier.hkuros155039-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61849144830&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue1en_HK
dc.identifier.spage24en_HK
dc.identifier.epage31en_HK
dc.identifier.isiWOS:000262659700005-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectDevelopment and clinical evaluation of peptide vaccines for immunotherapy of Epstein-Barr virus-positive nasopharyngeal carcinoma-
dc.relation.projectInstitute of molecular technology for drug discovery and synthesis-
dc.identifier.scopusauthoridCheung, WH=36152058800en_HK
dc.identifier.scopusauthoridChan, VSF=35200370000en_HK
dc.identifier.scopusauthoridPang, HW=7102237542en_HK
dc.identifier.scopusauthoridWong, MK=7403908449en_HK
dc.identifier.scopusauthoridGuo, ZH=7404658303en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridLin, CL=27169299500en_HK
dc.identifier.scopusauthoridYu, WY=7403913673en_HK

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