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Article: XIAP-associated factor 1 interacts with and attenuates the trans-activity of four and a Half LIM protein 2

TitleXIAP-associated factor 1 interacts with and attenuates the trans-activity of four and a Half LIM protein 2
Authors
KeywordsFHL2
Gastric cancer
XAF1
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
Citation
Molecular Carcinogenesis, 2011, v. 50 n. 3, p. 199-207 How to Cite?
AbstractXIAP-associated factor 1(XAF1) is a tumor suppressor with its functional mechanisms not fully understood. The zinc-finger cluster located at the N-terminus is the only domain structure. Four and a half LIM domain protein 2 (FHL2) also contains a tandem zinc finger structure, and its protein functions as an important adaptor and modifier in protein-protein interactions. Both of their structures are relatively simple, while the association between them is still unclear. In this study, we detected the interaction between XAF1 and FHL2 by using the yeast two-hybrid system. We identified FHL2 as a XAF1 binding protein. Furthermore, both XAF1 and FHL2 localized to the cytoplasm, mitochondria, and nucleus of gastric cancer cells. Over-expression of XAF1 excluded FHL2 from the nucleus and suppressed the trans-activity of FHL2 in stimulating the transcriptional activities of β-catenin and AP-1. In conclusion, our findings unraveled an antagonistic mechanism between a tumor suppressor and an oncoprotein in cancer cells. © 2010 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/132467
ISSN
2015 Impact Factor: 4.722
2015 SCImago Journal Rankings: 1.393
ISI Accession Number ID
Funding AgencyGrant Number
Simon KY Lee endowed professorship Research Fund
Gordon Chiu Stomach Cancer Research Fund
University Research Council200711159147
National Basic Research Program of China (973 Program)2010CB529306
Funding Information:

This study is supported by the Simon KY Lee endowed professorship Research Fund, Gordon Chiu Stomach Cancer Research Fund, and University Research Council Seed Basic Research Scheme (200711159147) and National Basic Research Program of China (973 Program), 2010CB529306. We thank Professor Kenneth W. Kinzler, Ludwig Center at Johns Hopkins University to kindly provide us the pTOPFLASH plasmid.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Wen_HK
dc.contributor.authorYang, Yen_HK
dc.contributor.authorJiang, Ben_HK
dc.contributor.authorPeng, Jen_HK
dc.contributor.authorTu, Sen_HK
dc.contributor.authorSardet, Cen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorPang, Ren_HK
dc.contributor.authorHung, IFen_HK
dc.contributor.authorTan, VPYen_HK
dc.contributor.authorLam, CSen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorWong, BCen_HK
dc.date.accessioned2011-03-28T09:25:00Z-
dc.date.available2011-03-28T09:25:00Z-
dc.date.issued2011en_HK
dc.identifier.citationMolecular Carcinogenesis, 2011, v. 50 n. 3, p. 199-207en_HK
dc.identifier.issn0899-1987en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132467-
dc.description.abstractXIAP-associated factor 1(XAF1) is a tumor suppressor with its functional mechanisms not fully understood. The zinc-finger cluster located at the N-terminus is the only domain structure. Four and a half LIM domain protein 2 (FHL2) also contains a tandem zinc finger structure, and its protein functions as an important adaptor and modifier in protein-protein interactions. Both of their structures are relatively simple, while the association between them is still unclear. In this study, we detected the interaction between XAF1 and FHL2 by using the yeast two-hybrid system. We identified FHL2 as a XAF1 binding protein. Furthermore, both XAF1 and FHL2 localized to the cytoplasm, mitochondria, and nucleus of gastric cancer cells. Over-expression of XAF1 excluded FHL2 from the nucleus and suppressed the trans-activity of FHL2 in stimulating the transcriptional activities of β-catenin and AP-1. In conclusion, our findings unraveled an antagonistic mechanism between a tumor suppressor and an oncoprotein in cancer cells. © 2010 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/en_HK
dc.relation.ispartofMolecular Carcinogenesisen_HK
dc.subjectFHL2en_HK
dc.subjectGastric canceren_HK
dc.subjectXAF1en_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Nucleus - metabolismen_HK
dc.subject.meshCytoplasm - metabolismen_HK
dc.subject.meshFluorescent Antibody Techniqueen_HK
dc.subject.meshHomeodomain Proteins - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoprecipitationen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteins - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshLIM-Homeodomain Proteinsen_HK
dc.subject.meshLuciferases - metabolismen_HK
dc.subject.meshMitochondria - metabolismen_HK
dc.subject.meshMuscle Proteins - genetics - metabolismen_HK
dc.subject.meshNeoplasm Proteins - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshRNA, Small Interfering - geneticsen_HK
dc.subject.meshStomach Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshTrans-Activatorsen_HK
dc.subject.meshTranscription Factor AP-1 - genetics - metabolismen_HK
dc.subject.meshTranscription Factors - genetics - metabolismen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.subject.meshTranscriptional Activationen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshTwo-Hybrid System Techniquesen_HK
dc.subject.meshbeta Catenin - genetics - metabolismen_HK
dc.titleXIAP-associated factor 1 interacts with and attenuates the trans-activity of four and a Half LIM protein 2en_HK
dc.typeArticleen_HK
dc.identifier.emailPang, R: robertap@hku.hken_HK
dc.identifier.emailHung, IF: ivanhung@hkucc.hku.hken_HK
dc.identifier.emailTan, VPY: vpytan@hku.hken_HK
dc.identifier.emailWang, J: jidewang@gmail.comen_HK
dc.identifier.emailWong, BC: bcywong@hku.hken_HK
dc.identifier.authorityPang, R=rp00274en_HK
dc.identifier.authorityHung, IF=rp00508en_HK
dc.identifier.authorityTan, VPY=rp01458en_HK
dc.identifier.authorityWang, J=rp00491en_HK
dc.identifier.authorityWong, BC=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/mc.20705en_HK
dc.identifier.pmid21104993-
dc.identifier.scopuseid_2-s2.0-79951816405en_HK
dc.identifier.hkuros185099-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79951816405&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue3en_HK
dc.identifier.spage199en_HK
dc.identifier.epage207en_HK
dc.identifier.eissn1098-2744-
dc.identifier.isiWOS:000288176800006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, W=7409428339en_HK
dc.identifier.scopusauthoridYang, Y=36124264300en_HK
dc.identifier.scopusauthoridJiang, B=34770534200en_HK
dc.identifier.scopusauthoridPeng, J=12796973500en_HK
dc.identifier.scopusauthoridTu, S=7202726555en_HK
dc.identifier.scopusauthoridSardet, C=7005616535en_HK
dc.identifier.scopusauthoridZhang, Y=36129128700en_HK
dc.identifier.scopusauthoridPang, R=7004376659en_HK
dc.identifier.scopusauthoridHung, IF=7006103457en_HK
dc.identifier.scopusauthoridTan, VPY=24449627600en_HK
dc.identifier.scopusauthoridLam, CS=35332626500en_HK
dc.identifier.scopusauthoridWang, J=35309087500en_HK
dc.identifier.scopusauthoridWong, BC=7402023340en_HK

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