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Article: Electrospinning, characterization and in vitro biological evaluation of nanocomposite fibers containing carbonated hydroxyapatite nanoparticles

TitleElectrospinning, characterization and in vitro biological evaluation of nanocomposite fibers containing carbonated hydroxyapatite nanoparticles
Authors
Issue Date2010
PublisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMM
Citation
Biomedical Materials, 2010, v. 5 n. 5 How to Cite?
AbstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibers containing carbonated hydroxyapatite (CHA) nanoparticles with different CHA amounts (5, 10 and 15 wt%) were electrospun with the aid of ultrasonic power for dispersing the nanoparticles. Scanning electron microscopy and energy-dispersive x-ray spectroscopy results showed that the distribution of CHA within the CHA/PHBV nanocomposite fibers was homogeneous when the CHA content was 10 wt%. Slight particle agglomeration occurred when the CHA content was 15 wt%. The diameters of the electrospun CHA/PHBV nanocomposite fibers and PHBV polymer fibers were around 3 μm. Fourier transform infrared spectroscopic analysis further confirmed the presence of CHA in CHA/PHBV nanocomposite fibers. Both PHBV and CHA/PHBV fibrous membranes exhibited similar tensile properties. Compared with PHBV solvent-cast film, the PHBV fibrous membrane was hydrophobic but the incorporation of CHA nanoparticles dramatically enhanced its wettability. In vitro studies revealed that both types of electrospun fibrous membranes (PHBV and CHA/PHBV) supported the proliferation of human osteoblastic cells (SaOS-2). The alkaline phosphatase activity of SaOS-2 cells seeded on the CHA/PHBV fibrous membranes was higher than that of the cells seeded on the PHBV fibrous membranes after 14 days of cell culture. The electrospun CHA/PHBV nanocomposite fibrous membranes show promises for bone tissue engineering applications. © 2010 IOP Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/132153
ISSN
2015 Impact Factor: 3.361
2015 SCImago Journal Rankings: 0.936
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU 7176/08E
Funding Information:

This work was supported by a GRF grant (HKU 7176/08E) from the Research Grants Council of Hong Kong. The authors acknowledge the assistance provided by technical staff in the Department of Mechanical Engineering and the Department of Orthopaedics and Traumatology, HKU.

References

 

DC FieldValueLanguage
dc.contributor.authorTong, HWen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorLi, ZYen_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2011-03-08T06:50:35Z-
dc.date.available2011-03-08T06:50:35Z-
dc.date.issued2010en_HK
dc.identifier.citationBiomedical Materials, 2010, v. 5 n. 5en_HK
dc.identifier.issn1748-6041en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132153-
dc.description.abstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibers containing carbonated hydroxyapatite (CHA) nanoparticles with different CHA amounts (5, 10 and 15 wt%) were electrospun with the aid of ultrasonic power for dispersing the nanoparticles. Scanning electron microscopy and energy-dispersive x-ray spectroscopy results showed that the distribution of CHA within the CHA/PHBV nanocomposite fibers was homogeneous when the CHA content was 10 wt%. Slight particle agglomeration occurred when the CHA content was 15 wt%. The diameters of the electrospun CHA/PHBV nanocomposite fibers and PHBV polymer fibers were around 3 μm. Fourier transform infrared spectroscopic analysis further confirmed the presence of CHA in CHA/PHBV nanocomposite fibers. Both PHBV and CHA/PHBV fibrous membranes exhibited similar tensile properties. Compared with PHBV solvent-cast film, the PHBV fibrous membrane was hydrophobic but the incorporation of CHA nanoparticles dramatically enhanced its wettability. In vitro studies revealed that both types of electrospun fibrous membranes (PHBV and CHA/PHBV) supported the proliferation of human osteoblastic cells (SaOS-2). The alkaline phosphatase activity of SaOS-2 cells seeded on the CHA/PHBV fibrous membranes was higher than that of the cells seeded on the PHBV fibrous membranes after 14 days of cell culture. The electrospun CHA/PHBV nanocomposite fibrous membranes show promises for bone tissue engineering applications. © 2010 IOP Publishing Ltd.en_HK
dc.languageeng-
dc.publisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMMen_HK
dc.relation.ispartofBiomedical Materialsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsBiomedical Materials (Bristol). Copyright © Institute of Physics Publishing Ltd.-
dc.subject.meshBone and Bones - cytology - metabolism-
dc.subject.meshCarbonates - chemistry-
dc.subject.meshDurapatite - chemistry-
dc.subject.meshNanoparticles - chemistry-
dc.subject.meshNanostructures - chemistry - ultrastructure-
dc.titleElectrospinning, characterization and in vitro biological evaluation of nanocomposite fibers containing carbonated hydroxyapatite nanoparticlesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1748-6041&volume=5&issue=5, article no. 054111&spage=&epage=&date=2010&atitle=Electrospinning,+characterization+and+in+vitro+biological+evaluation+of+nanocomposite+fibers+containing+carbonated+hydroxyapatite+nanoparticles-
dc.identifier.emailWang, M:memwang@hku.hken_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.authorityWang, M=rp00185en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1088/1748-6041/5/5/054111en_HK
dc.identifier.pmid20876957-
dc.identifier.scopuseid_2-s2.0-78649266025en_HK
dc.identifier.hkuros183162-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649266025&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue5en_HK
dc.identifier.eissn1748-605X-
dc.identifier.isiWOS:000282277300012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTong, HW=23476100900en_HK
dc.identifier.scopusauthoridWang, M=15749714100en_HK
dc.identifier.scopusauthoridLi, ZY=35727919500en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK

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