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Article: Protective effect of bilberry (Vaccinium myrtillus L.) extracts on cultured Human Corneal Limbal Epithelial Cells (HCLEC)

TitleProtective effect of bilberry (Vaccinium myrtillus L.) extracts on cultured Human Corneal Limbal Epithelial Cells (HCLEC)
Authors
KeywordsCell cycle
Cell viability
Glycosaminoglycans
Hyaluronic acid
Vaccinium myrtillus L.
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/12567
Citation
Phytotherapy Research, 2010, v. 24 n. 4, p. 520-524 How to Cite?
AbstractThe use of bilberry (Vaccinium myrtillus L.) as a food and medicine for improving human vision has a long history all over the world. However, there is lack of convincing evidence from rigorous clinical trials or scientific research. This study investigated the effects of different concentrations of bilberry extracts on the cell viability, cell cycle and the expression of hyaluronic acid and glycosaminoglycans of cultured human corneal limbal epithelial cells. The data showed that bilberry extracts had no cytotoxicity to the corneal limbal epithelial cells at a wide range of concentrations (10 -9-10 -4 M, equalized to the content of cyanidin-3-O-glucoside). Bilberry extract (10 -6, 10 -5 and 10 -4 M) increased cell viability after 48 h incubation. The number of cells decreased in G 0/G 1 phase and increased prominently in S and G 2/M phases after treatment with bilberry extracts at a high concentration (10 -4 M). The expression of glycosaminoglycans increased prominently after incubation with bilberry extracts (10 -7 and 10 -4 M) for 48 h while no significant changes were observed for the expression of hyaluronic acid. The results indicated that bilberry extract may be beneficial for the physiological renewal and homeostasis of corneal epithelial cells. Copyright © 2010 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/130088
ISSN
2015 Impact Factor: 2.694
2015 SCImago Journal Rankings: 0.842
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong20005126
10208016
Funding Information:

This research work was supported by an industrial Support Fund (No. 20005126) and research fund (No: 10208016) from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorSong, Jen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorGe, Jen_HK
dc.contributor.authorDuan, Yen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorShaw, PCen_HK
dc.contributor.authorNg, TBen_HK
dc.contributor.authorTsui, KCen_HK
dc.contributor.authorZhuo, Yen_HK
dc.contributor.authorZhang, KYen_HK
dc.date.accessioned2010-12-23T08:46:46Z-
dc.date.available2010-12-23T08:46:46Z-
dc.date.issued2010en_HK
dc.identifier.citationPhytotherapy Research, 2010, v. 24 n. 4, p. 520-524en_HK
dc.identifier.issn0951-418Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/130088-
dc.description.abstractThe use of bilberry (Vaccinium myrtillus L.) as a food and medicine for improving human vision has a long history all over the world. However, there is lack of convincing evidence from rigorous clinical trials or scientific research. This study investigated the effects of different concentrations of bilberry extracts on the cell viability, cell cycle and the expression of hyaluronic acid and glycosaminoglycans of cultured human corneal limbal epithelial cells. The data showed that bilberry extracts had no cytotoxicity to the corneal limbal epithelial cells at a wide range of concentrations (10 -9-10 -4 M, equalized to the content of cyanidin-3-O-glucoside). Bilberry extract (10 -6, 10 -5 and 10 -4 M) increased cell viability after 48 h incubation. The number of cells decreased in G 0/G 1 phase and increased prominently in S and G 2/M phases after treatment with bilberry extracts at a high concentration (10 -4 M). The expression of glycosaminoglycans increased prominently after incubation with bilberry extracts (10 -7 and 10 -4 M) for 48 h while no significant changes were observed for the expression of hyaluronic acid. The results indicated that bilberry extract may be beneficial for the physiological renewal and homeostasis of corneal epithelial cells. Copyright © 2010 John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/12567en_HK
dc.relation.ispartofPhytotherapy Researchen_HK
dc.rightsPhytotherapy Research. Copyright © John Wiley & Sons Ltd.-
dc.subjectCell cycleen_HK
dc.subjectCell viabilityen_HK
dc.subjectGlycosaminoglycansen_HK
dc.subjectHyaluronic aciden_HK
dc.subjectVaccinium myrtillus L.en_HK
dc.titleProtective effect of bilberry (Vaccinium myrtillus L.) extracts on cultured Human Corneal Limbal Epithelial Cells (HCLEC)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0951-418X&volume=24&issue=4&spage=520&epage=540&date=2010&atitle=Protective+effect+of+bilberry+(Vaccinium+myrtillus+L.)+extracts+on+cultured+human+corneal+limbal+epithelial+cells+(HCLEC)-
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailZhang, KY: ybzhang@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authorityZhang, KY=rp01410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ptr.2974en_HK
dc.identifier.pmid20077406-
dc.identifier.scopuseid_2-s2.0-77951590163en_HK
dc.identifier.hkuros177275en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951590163&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue4en_HK
dc.identifier.spage520en_HK
dc.identifier.epage524en_HK
dc.identifier.isiWOS:000276802500009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSong, J=24339343800en_HK
dc.identifier.scopusauthoridLi, Y=26643217800en_HK
dc.identifier.scopusauthoridGe, J=25421653600en_HK
dc.identifier.scopusauthoridDuan, Y=24340817100en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridShaw, PC=35599523600en_HK
dc.identifier.scopusauthoridNg, TB=35311803300en_HK
dc.identifier.scopusauthoridTsui, KC=36673959100en_HK
dc.identifier.scopusauthoridZhuo, Y=7005221373en_HK
dc.identifier.scopusauthoridZhang, KY=23483121900en_HK

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