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Article: Protein expression during the embryonic development of a gastropod

TitleProtein expression during the embryonic development of a gastropod
Authors
Keywords2-DE
Animal proteomics
Apple snail
de novo sequencing
Embryonic development
Gastropod
Issue Date2010
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2010, v. 10 n. 14, p. 2701-2711 How to Cite?
AbstractDespite the potential use of gastropod embryos in basic and applied research, little is known about their protein expression. We examined, for the first time, changes in proteomic profile during embryonic development of Pomacea canaliculata from an embryo without a shell (stage II) to an embryo with a fully formed shell (stage III) to understand the roles that proteins play in critical developmental events, such as the formation of shell, operculum and heart, and the differentiation of head and foot. To analyze protein expression during development, we used 2-DE to detect, MS to analyze, and de novo peptide sequencing followed by MS-BLAST to identify the proteins. The de novo cross-species protein identification method was adopted because of a lack of genomic and proteomic data in the whole class of Gastropoda. 2-DE detected approximately 700 protein spots. Among the 125 spots that were abundant, 52% were identified, a marked improvement over the conventional direct MS-BLAST method. These proteins function in perivitelline fluid utilization, shell formation, protein synthesis and folding, and cell cycle and cell fate determination, providing evidence to support that this embryonic period is a period of dynamic protein synthesis and metabolism. The data shall provide a basis for further studies of how gastropod embryos respond to natural and human-induced changes in the environment. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/130016
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
Funding AgencyGrant Number
HKBUHKBU FRG/08-09/II-08
AoE/P-04/04-2-II
Funding Information:

The authors thank Professor Andrej Shevchenko for his guidance on de novo protein identification, Pak Ki Wong for collecting snails from the field, Yin Ki Tam and Jason Tam for conducting mass spectrometry analyses, and two anonymous reviewers for critical comments. This study was supported by a HKBU postgraduate scholarship to J. S., a grant (HKBU FRG/08-09/II-08) to J. W. Q., and a grant (AoE/P-04/04-2-II) to P. Y. Q.

References

 

DC FieldValueLanguage
dc.contributor.authorSun, Jen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorThiyagarajan, Ven_HK
dc.contributor.authorQian, PYen_HK
dc.contributor.authorQiu, JWen_HK
dc.date.accessioned2010-12-23T08:45:37Z-
dc.date.available2010-12-23T08:45:37Z-
dc.date.issued2010en_HK
dc.identifier.citationProteomics, 2010, v. 10 n. 14, p. 2701-2711en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/130016-
dc.description.abstractDespite the potential use of gastropod embryos in basic and applied research, little is known about their protein expression. We examined, for the first time, changes in proteomic profile during embryonic development of Pomacea canaliculata from an embryo without a shell (stage II) to an embryo with a fully formed shell (stage III) to understand the roles that proteins play in critical developmental events, such as the formation of shell, operculum and heart, and the differentiation of head and foot. To analyze protein expression during development, we used 2-DE to detect, MS to analyze, and de novo peptide sequencing followed by MS-BLAST to identify the proteins. The de novo cross-species protein identification method was adopted because of a lack of genomic and proteomic data in the whole class of Gastropoda. 2-DE detected approximately 700 protein spots. Among the 125 spots that were abundant, 52% were identified, a marked improvement over the conventional direct MS-BLAST method. These proteins function in perivitelline fluid utilization, shell formation, protein synthesis and folding, and cell cycle and cell fate determination, providing evidence to support that this embryonic period is a period of dynamic protein synthesis and metabolism. The data shall provide a basis for further studies of how gastropod embryos respond to natural and human-induced changes in the environment. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subject2-DEen_HK
dc.subjectAnimal proteomicsen_HK
dc.subjectApple snailen_HK
dc.subjectde novo sequencingen_HK
dc.subjectEmbryonic developmenten_HK
dc.subjectGastropoden_HK
dc.titleProtein expression during the embryonic development of a gastropoden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9861&volume=10&issue=14&spage=2701&epage=2711&date=2010&atitle=Protein+expression+during+the+embryonic+development+of+a+gastropoden_US
dc.identifier.emailThiyagarajan, V: rajan@hkucc.hku.hken_HK
dc.identifier.authorityThiyagarajan, V=rp00796en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200900846en_HK
dc.identifier.pmid20455212-
dc.identifier.scopuseid_2-s2.0-77954709515en_HK
dc.identifier.hkuros177919en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954709515&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue14en_HK
dc.identifier.spage2701en_HK
dc.identifier.epage2711en_HK
dc.identifier.isiWOS:000280615200011-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridSun, J=8734512600en_HK
dc.identifier.scopusauthoridZhang, Y=36038958500en_HK
dc.identifier.scopusauthoridThiyagarajan, V=6602476830en_HK
dc.identifier.scopusauthoridQian, PY=35240648600en_HK
dc.identifier.scopusauthoridQiu, JW=7403309861en_HK

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