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Article: Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay

TitleOptimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay
Authors
Garcia, JMLagarde, NMa, ESKde Jong, MDPeiris, JSM
KeywordsDiagnosis
H5N1
Influenza
Pseudoparticle
Pseudotyped lentiviral particles
Serology
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2010, v. 47 n. 1, p. 29-33 How to Cite?
DOI: http://dx.doi.org/10.1016/j.jcv.2009.10.009
AbstractBackground: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). Objective: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. Study design: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. Results: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. Conclusion: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/129963
ISSN
1386-6532
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.344
ISI Accession Number ID
WOS:000273111200007
Funding AgencyGrant Number
University Grants Committee of the Hong Kong Special Administrative Region, ChinaAoE/M-12/06
French Ministry of Health, RESPARI Pasteur network
Li Ka Shing Foundation
Funding Information:

This work was supported by the University Grants Committee of the Hong Kong Special Administrative Region, China (Project No AoE/M-12/06), the French Ministry of Health, RESPARI Pasteur network and the Li Ka Shing Foundation

References
References in Scopus
Grants
Control of Pandemic and Inter-pandemic Influenza

 

DC FieldValueLanguage
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorLagarde, Nen_HK
dc.contributor.authorMa, ESKen_HK
dc.contributor.authorde Jong, MDen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-12-23T08:44:51Z-
dc.date.available2010-12-23T08:44:51Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Virology, 2010, v. 47 n. 1, p. 29-33en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129963-
dc.description.abstractBackground: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). Objective: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. Study design: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. Results: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. Conclusion: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDiagnosisen_HK
dc.subjectH5N1en_HK
dc.subjectInfluenzaen_HK
dc.subjectPseudoparticleen_HK
dc.subjectPseudotyped lentiviral particlesen_HK
dc.subjectSerologyen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - immunology-
dc.subject.meshInfluenza, Human - diagnosis - immunology-
dc.subject.meshLentivirus - genetics-
dc.subject.meshNeutralization Tests - methods-
dc.subject.meshVirion - genetics-
dc.titleOptimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=47&issue=1&spage=29&epage=33&date=2010&atitle=Optimization+and+evaluation+of+an+influenza+A+(H5)+pseudotyped+lentiviral+particle-based+serological+assay-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jcv.2009.10.009en_HK
dc.identifier.pmid19897409en_HK
dc.identifier.scopuseid_2-s2.0-72049126376en_HK
dc.identifier.hkuros178058en_US
dc.identifier.hkuros162416-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72049126376&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue1en_HK
dc.identifier.spage29en_HK
dc.identifier.epage33en_HK
dc.identifier.isiWOS:000273111200007-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridLagarde, N=16203012000en_HK
dc.identifier.scopusauthoridMa, ESK=24725277400en_HK
dc.identifier.scopusauthoridde Jong, MD=7201366838en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.citeulike6198222-
dc.identifier.issnl1386-6532-

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