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Article: Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay

TitleOptimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay
Authors
KeywordsDiagnosis
H5N1
Influenza
Pseudoparticle
Pseudotyped lentiviral particles
Serology
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2010, v. 47 n. 1, p. 29-33 How to Cite?
Abstract
Background: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). Objective: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. Study design: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. Results: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. Conclusion: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/129963
ISSN
2013 Impact Factor: 3.466
2013 SCImago Journal Rankings: 1.812
ISI Accession Number ID
Funding AgencyGrant Number
University Grants Committee of the Hong Kong Special Administrative Region, ChinaAoE/M-12/06
French Ministry of Health, RESPARI Pasteur network
Li Ka Shing Foundation
Funding Information:

This work was supported by the University Grants Committee of the Hong Kong Special Administrative Region, China (Project No AoE/M-12/06), the French Ministry of Health, RESPARI Pasteur network and the Li Ka Shing Foundation

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorLagarde, Nen_HK
dc.contributor.authorMa, ESKen_HK
dc.contributor.authorde Jong, MDen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-12-23T08:44:51Z-
dc.date.available2010-12-23T08:44:51Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Virology, 2010, v. 47 n. 1, p. 29-33en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129963-
dc.description.abstractBackground: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). Objective: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. Study design: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. Results: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. Conclusion: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectDiagnosisen_HK
dc.subjectH5N1en_HK
dc.subjectInfluenzaen_HK
dc.subjectPseudoparticleen_HK
dc.subjectPseudotyped lentiviral particlesen_HK
dc.subjectSerologyen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - immunology-
dc.subject.meshInfluenza, Human - diagnosis - immunology-
dc.subject.meshLentivirus - genetics-
dc.subject.meshNeutralization Tests - methods-
dc.subject.meshVirion - genetics-
dc.titleOptimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=47&issue=1&spage=29&epage=33&date=2010&atitle=Optimization+and+evaluation+of+an+influenza+A+(H5)+pseudotyped+lentiviral+particle-based+serological+assay-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jcv.2009.10.009en_HK
dc.identifier.pmid19897409en_HK
dc.identifier.scopuseid_2-s2.0-72049126376en_HK
dc.identifier.hkuros178058en_US
dc.identifier.hkuros162416-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72049126376&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue1en_HK
dc.identifier.spage29en_HK
dc.identifier.epage33en_HK
dc.identifier.isiWOS:000273111200007-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridLagarde, N=16203012000en_HK
dc.identifier.scopusauthoridMa, ESK=24725277400en_HK
dc.identifier.scopusauthoridde Jong, MD=7201366838en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.citeulike6198222-

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