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Article: Kinetics of neutralizing antibodies in patients naturally infected by H5N1 virus.

TitleKinetics of neutralizing antibodies in patients naturally infected by H5N1 virus.
Authors
Issue Date2010
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2010, v. 5 n. 5, p. e10864 How to Cite?
AbstractBACKGROUND: Little is known about the kinetics of anti-H5 neutralizing antibodies in naturally H5N1-infected patients with severe clinical illness or asymptomatic infection. METHODS: Using H5N1 microneutralisation (MN) and H5-pseudotype particle-based microneutralisation assays (H5pp) we analyzed sera sequentially obtained from 11 severely ill patients diagnosed by RT-PCR (follow-up range 1-139 weeks of disease onset) and 31 asymptomatically infected individuals detected in a sero-epidemiological study after exposure to H5N1 virus (follow-up range: 1-2 month-11 months after exposure). RESULTS: Of 44 sera from 11 patients with H5N1 disease, 70% tested positive by MN (antibody titre > or = 80) after 2 weeks and 100% were positive by 3 weeks after disease onset. The geometric mean MN titers in severely ill patients were 540 at 1-2 months and 173 at 10-12 months and thus were higher than the titers from asymptomatic individuals (149 at 1-2 months, 62.2 at 10-12 months). Fractional polynomial regression analysis demonstrated that in all severely ill patients, positive titers persisted beyond 2 years of disease onset, while 10 of 23 sera collected 10-11 months after exposure in asymptomatically infected individuals tested negative. CONCLUSIONS: Our results indicate that people with asymptomatic H5N1 infection have lower H5N1 antibody titres compared to those with severe illness and that in many asymptomatically infected patients the antibody titer decreased to levels below the threshold of positivity within one year. These data are essential for the design and interpretation of sero-epidemiological studies.
Persistent Identifierhttp://hdl.handle.net/10722/129962
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
AFD
Office of the Assistant Secretary for Preparedness and Response within the U.S. Department of Health and Human Services
University Grants Committee, Hong KongAoE/M-12/06
Wellcome Trust, UK
Funding Information:

This work was supported by a grant from the AFD (French Agency for Development, SISEA project, RESPARI program, http://www.pasteurinternational.org/ip/easysite/pasteur-international-en/scientific-activities/projects/all-our-projects/sisea), by the Office of the Assistant Secretary for Preparedness and Response within the U.S. Department of Health and Human Services, by the Area of Excellence Scheme of the University Grants Committee, Hong Kong (Grant AoE/M-12/06), and by the Wellcome Trust, UK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Grants

 

DC FieldValueLanguage
dc.contributor.authorBuchy, Pen_HK
dc.contributor.authorVong, Sen_HK
dc.contributor.authorChu, Sen_HK
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorHien, TTen_HK
dc.contributor.authorHien, VMen_HK
dc.contributor.authorChanna, Men_HK
dc.contributor.authorHa, Qen_HK
dc.contributor.authorChau, NVen_HK
dc.contributor.authorSimmons, Cen_HK
dc.contributor.authorFarrar, JJen_HK
dc.contributor.authorPeiris, Men_HK
dc.contributor.authorde Jong, MDen_HK
dc.date.accessioned2010-12-23T08:44:50Z-
dc.date.available2010-12-23T08:44:50Z-
dc.date.issued2010en_HK
dc.identifier.citationPlos One, 2010, v. 5 n. 5, p. e10864en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129962-
dc.description.abstractBACKGROUND: Little is known about the kinetics of anti-H5 neutralizing antibodies in naturally H5N1-infected patients with severe clinical illness or asymptomatic infection. METHODS: Using H5N1 microneutralisation (MN) and H5-pseudotype particle-based microneutralisation assays (H5pp) we analyzed sera sequentially obtained from 11 severely ill patients diagnosed by RT-PCR (follow-up range 1-139 weeks of disease onset) and 31 asymptomatically infected individuals detected in a sero-epidemiological study after exposure to H5N1 virus (follow-up range: 1-2 month-11 months after exposure). RESULTS: Of 44 sera from 11 patients with H5N1 disease, 70% tested positive by MN (antibody titre > or = 80) after 2 weeks and 100% were positive by 3 weeks after disease onset. The geometric mean MN titers in severely ill patients were 540 at 1-2 months and 173 at 10-12 months and thus were higher than the titers from asymptomatic individuals (149 at 1-2 months, 62.2 at 10-12 months). Fractional polynomial regression analysis demonstrated that in all severely ill patients, positive titers persisted beyond 2 years of disease onset, while 10 of 23 sera collected 10-11 months after exposure in asymptomatically infected individuals tested negative. CONCLUSIONS: Our results indicate that people with asymptomatic H5N1 infection have lower H5N1 antibody titres compared to those with severe illness and that in many asymptomatically infected patients the antibody titer decreased to levels below the threshold of positivity within one year. These data are essential for the design and interpretation of sero-epidemiological studies.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPloS oneen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntibodies, Neutralizing - immunology-
dc.subject.meshAntibodies, Viral - immunology-
dc.subject.meshKinetics-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - classification - immunology-
dc.subject.meshInfluenza, Human - blood - immunology-
dc.titleKinetics of neutralizing antibodies in patients naturally infected by H5N1 virus.en_HK
dc.typeArticleen_HK
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, M=rp00410en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0010864en_HK
dc.identifier.pmid20532246-
dc.identifier.pmcidPMC2879427-
dc.identifier.scopuseid_2-s2.0-77956503084en_HK
dc.identifier.hkuros178065en_US
dc.identifier.volume5en_HK
dc.identifier.issue5en_HK
dc.identifier.spagee10864en_HK
dc.identifier.epagee10864en_HK
dc.identifier.isiWOS:000278221900014-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridBuchy, P=15019240000en_HK
dc.identifier.scopusauthoridVong, S=6603420595en_HK
dc.identifier.scopusauthoridChu, S=36243207900en_HK
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridHien, TT=7005271267en_HK
dc.identifier.scopusauthoridHien, VM=10043209900en_HK
dc.identifier.scopusauthoridChanna, M=36097732200en_HK
dc.identifier.scopusauthoridHa, Q=7101767746en_HK
dc.identifier.scopusauthoridChau, NV=23007606000en_HK
dc.identifier.scopusauthoridSimmons, C=7103173872en_HK
dc.identifier.scopusauthoridFarrar, JJ=7103292979en_HK
dc.identifier.scopusauthoridPeiris, M=7005486823en_HK
dc.identifier.scopusauthoridde Jong, MD=7201366838en_HK

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