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Conference Paper: Sonoporation-induced apoptosis and cell cycle arrest: Initial findings

TitleSonoporation-induced apoptosis and cell cycle arrest: Initial findings
Authors
KeywordsApoptosis
Cell cycle
Sonoporation
Ultrasound
Issue Date2011
PublisherAmerican Institute of Physics. The Journal's web site is located at http://proceedings.aip.org/
Citation
The 10th International Symposium on Therapeutic Ultrasound (ISTU 2010), Tokyo, Japan, 9-12 June 2010. In Aip Conference Proceedings, 2011, v. 1359, p. 312-317 How to Cite?
AbstractSonoporation is known to be able to temporarily permeabilize cells, but during this process it may have traumatic impact on cell viability. In this work, we found that sonoporation may induce apoptosis and G 2/M-phase cell cycle arrest in some cells hours after ultrasonic exposure in vitro. Methods: Suspensions of HL-60 leukemia cells were prepared (10 6 cells/ml), and a 1% v/v microbubble solution was added to induce sonoporation during ultrasound exposure. They were then placed 7cm away from a 2.54cm-diameter, 1MHz unfocused ultrasound probe, and these samples were insonated for 1 min with ultrasound pulses (10% duty cycle, 1kHz pulse repetition frequency). In this study, two levels of peak negative ultrasound pressure were used: 0.3MPa and 0.5MPa. After exposure, the cell suspensions were further incubated. They were harvested after 4h, 8h, 12h and 24h to analyze the cell-cycle distribution (sub-G 1, G 0/G 1, S, G 2/M) at these time points using propidium iodide staining and flow cytometry. Results: Some sonoporation-treated cells had undergone apoptosis by 4h, and the largest number of apoptotic cells (sub-G 1 phase) was observed after 12h (0.3 MPa group: 25.0%; 0.5 MPa group: 27.2%). Also, after experiencing sonoporation, some viable cells were stopped in the G 2/M phase without undergoing cytokinesis, and the maximum G 2/M population rise was seen after 12h (0.3 MPa group: +12.2%; 0.5 MPa group: +14.7%). This was accompanied by decreases in the populations of G 0/G 1-phase and S-phase. © 2011 American Institute of Physics.
Persistent Identifierhttp://hdl.handle.net/10722/129660
ISSN
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhong, Wen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorWan, JMFen_HK
dc.contributor.authorYu, ACHen_HK
dc.date.accessioned2010-12-23T08:40:47Z-
dc.date.available2010-12-23T08:40:47Z-
dc.date.issued2011en_HK
dc.identifier.citationThe 10th International Symposium on Therapeutic Ultrasound (ISTU 2010), Tokyo, Japan, 9-12 June 2010. In Aip Conference Proceedings, 2011, v. 1359, p. 312-317en_HK
dc.identifier.issn0094-243Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/129660-
dc.description.abstractSonoporation is known to be able to temporarily permeabilize cells, but during this process it may have traumatic impact on cell viability. In this work, we found that sonoporation may induce apoptosis and G 2/M-phase cell cycle arrest in some cells hours after ultrasonic exposure in vitro. Methods: Suspensions of HL-60 leukemia cells were prepared (10 6 cells/ml), and a 1% v/v microbubble solution was added to induce sonoporation during ultrasound exposure. They were then placed 7cm away from a 2.54cm-diameter, 1MHz unfocused ultrasound probe, and these samples were insonated for 1 min with ultrasound pulses (10% duty cycle, 1kHz pulse repetition frequency). In this study, two levels of peak negative ultrasound pressure were used: 0.3MPa and 0.5MPa. After exposure, the cell suspensions were further incubated. They were harvested after 4h, 8h, 12h and 24h to analyze the cell-cycle distribution (sub-G 1, G 0/G 1, S, G 2/M) at these time points using propidium iodide staining and flow cytometry. Results: Some sonoporation-treated cells had undergone apoptosis by 4h, and the largest number of apoptotic cells (sub-G 1 phase) was observed after 12h (0.3 MPa group: 25.0%; 0.5 MPa group: 27.2%). Also, after experiencing sonoporation, some viable cells were stopped in the G 2/M phase without undergoing cytokinesis, and the maximum G 2/M population rise was seen after 12h (0.3 MPa group: +12.2%; 0.5 MPa group: +14.7%). This was accompanied by decreases in the populations of G 0/G 1-phase and S-phase. © 2011 American Institute of Physics.en_HK
dc.languageengen_US
dc.publisherAmerican Institute of Physics. The Journal's web site is located at http://proceedings.aip.org/-
dc.relation.ispartofAIP Conference Proceedingsen_HK
dc.rightsAIP Conference Proceedings. Copyright © American Institute of Physics.-
dc.rightsAfter publication: Copyright (year) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in (citation of published article) and may be found at (URL/link for published article abstract). Before publication: The following article has been submitted to/accepted by [Name of Journal]. After it is published, it will be found at (URL/link to the entry page of the journal).-
dc.subjectApoptosisen_HK
dc.subjectCell cycleen_HK
dc.subjectSonoporationen_HK
dc.subjectUltrasounden_HK
dc.titleSonoporation-induced apoptosis and cell cycle arrest: Initial findingsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.identifier.authorityYu, ACH=rp00657en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1063/1.3607924en_HK
dc.identifier.scopuseid_2-s2.0-80053651577en_HK
dc.identifier.hkuros176824en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053651577&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1359en_HK
dc.identifier.spage312en_HK
dc.identifier.epage317en_HK
dc.identifier.isiWOS:000295944900050-
dc.publisher.placeUnited Statesen_HK
dc.description.otherThe 10th International Symposium on Therapeutic Ultrasound (ISTU 2010), Tokyo, Japan, 9-12 June 2010. In Aip Conference Proceedings, 2011, v. 1359, p. 312-317-
dc.identifier.scopusauthoridZhong, W=46761637400en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK
dc.identifier.scopusauthoridYu, ACH=8699317700en_HK

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