Conference Paper: Factors affecting Candida albicans exoenzyme activities in HIV-infected patients

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Title	Factors affecting Candida albicans exoenzyme activities in HIV-infected patients
Authors	Tsang, PCS Thomas, S Perera, RAPM
Keywords	Medical sciences Dentistry
Issue Date	2010
Publisher	Sage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation	The 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795 [How to Cite?]
Abstract	The mortality and morbidity of HIV-infected patients have declined with the advent of highly active antiretroviral therapy (HAART). However, its effect on Candida infections remains inconclusive. Objectives: To determine the phospholipase, proteinase and haemoltyic activities of oral C. albicans in HIV-infected patients on HAART, and the factors affecting their activities. Methods: Altogether 32 C. albicans isolates were tested in vitro for phospholipase, proteinase and haemolytic activities using three different plate assays. Control group comprised of C. albicans collected from 16 healthy individuals. Simple regression was used to study the correlation of the enzymatic activities with gender, age, education level, income, HIV stage of infection, current medications, duration on HAART, history of oral candidiasis, HIV viral load, CD4 count and salivary flow rate. Results: Phospholipase and haemolytic activities were found to be significantly higher (p < 0.05) and proteinase activities were lower in the test group (p < 0.05). There was a significant correlation of the pH value of saliva with the phospholipase activities in the test group (R Sq Linear = 0.594). Patients taking abacavir, a nucleoside reverse transcriptase inhibitor (NRTI), were found to have higher proteinase activities (p < 0.05). However, haemolysin activities were significantly lower in patients taking abacavir (p < 0.05). The use of protease inhibitors ritonavir and lopinavir was also found to be associated with decreased haemolysin activities (p < 0.05). The lower proteinase activities in HIV-infected group could be due to the direct effect of protease inhibitors in HAART while the higher phospholipase and haemolytic activity in the HIV-infected group remains elusive. Conclusion: These finding provide evidence of altered extracellular enzyme activities in C. albicans isolates obtained from HIV patients on HAART, which may affect the infectivity of the Candida isolates.
Description	Session - Yeasts and Fungi
ISSN	0022-0345 2011 Impact Factor: 3.486 2011 SCImago Journal Rankings: 0.220

DC Field	Value
dc.contributor.author	Tsang, PCS
dc.contributor.author	Thomas, S
dc.contributor.author	Perera, RAPM
dc.date.accessioned	2010-12-23T08:39:48Z
dc.date.available	2010-12-23T08:39:48Z
dc.date.issued	2010
dc.description.abstract	The mortality and morbidity of HIV-infected patients have declined with the advent of highly active antiretroviral therapy (HAART). However, its effect on Candida infections remains inconclusive. Objectives: To determine the phospholipase, proteinase and haemoltyic activities of oral C. albicans in HIV-infected patients on HAART, and the factors affecting their activities. Methods: Altogether 32 C. albicans isolates were tested in vitro for phospholipase, proteinase and haemolytic activities using three different plate assays. Control group comprised of C. albicans collected from 16 healthy individuals. Simple regression was used to study the correlation of the enzymatic activities with gender, age, education level, income, HIV stage of infection, current medications, duration on HAART, history of oral candidiasis, HIV viral load, CD4 count and salivary flow rate. Results: Phospholipase and haemolytic activities were found to be significantly higher (p < 0.05) and proteinase activities were lower in the test group (p < 0.05). There was a significant correlation of the pH value of saliva with the phospholipase activities in the test group (R Sq Linear = 0.594). Patients taking abacavir, a nucleoside reverse transcriptase inhibitor (NRTI), were found to have higher proteinase activities (p < 0.05). However, haemolysin activities were significantly lower in patients taking abacavir (p < 0.05). The use of protease inhibitors ritonavir and lopinavir was also found to be associated with decreased haemolysin activities (p < 0.05). The lower proteinase activities in HIV-infected group could be due to the direct effect of protease inhibitors in HAART while the higher phospholipase and haemolytic activity in the HIV-infected group remains elusive. Conclusion: These finding provide evidence of altered extracellular enzyme activities in C. albicans isolates obtained from HIV patients on HAART, which may affect the infectivity of the Candida isolates.
dc.description	Session - Yeasts and Fungi
dc.description.other	The 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795
dc.identifier.citation	The 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795 [How to Cite?]
dc.identifier.hkuros	177464
dc.identifier.issn	0022-0345 2011 Impact Factor: 3.486 2011 SCImago Journal Rankings: 0.220
dc.identifier.issue	Spec. Iss B
dc.identifier.openurl
dc.identifier.uri	http://hdl.handle.net/10722/129600
dc.identifier.volume	89
dc.language	eng
dc.publisher	Sage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
dc.relation.ispartof	Journal of Dental Research
dc.rights	Journal of Dental Research. Copyright © Sage Publications, Inc..
dc.subject	Medical sciences
dc.subject	Dentistry
dc.title	Factors affecting Candida albicans exoenzyme activities in HIV-infected patients
dc.type	Conference_Paper

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Conference Paper: Factors affecting Candida albicans exoenzyme activities in HIV-infected patients

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