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Conference Paper: Factors affecting Candida albicans exoenzyme activities in HIV-infected patients

TitleFactors affecting Candida albicans exoenzyme activities in HIV-infected patients
Authors
KeywordsMedical sciences
Dentistry
Issue Date2010
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795 How to Cite?
Abstract
The mortality and morbidity of HIV-infected patients have declined with the advent of highly active antiretroviral therapy (HAART). However, its effect on Candida infections remains inconclusive. Objectives: To determine the phospholipase, proteinase and haemoltyic activities of oral C. albicans in HIV-infected patients on HAART, and the factors affecting their activities. Methods: Altogether 32 C. albicans isolates were tested in vitro for phospholipase, proteinase and haemolytic activities using three different plate assays. Control group comprised of C. albicans collected from 16 healthy individuals. Simple regression was used to study the correlation of the enzymatic activities with gender, age, education level, income, HIV stage of infection, current medications, duration on HAART, history of oral candidiasis, HIV viral load, CD4 count and salivary flow rate. Results: Phospholipase and haemolytic activities were found to be significantly higher (p < 0.05) and proteinase activities were lower in the test group (p < 0.05). There was a significant correlation of the pH value of saliva with the phospholipase activities in the test group (R Sq Linear = 0.594). Patients taking abacavir, a nucleoside reverse transcriptase inhibitor (NRTI), were found to have higher proteinase activities (p < 0.05). However, haemolysin activities were significantly lower in patients taking abacavir (p < 0.05). The use of protease inhibitors ritonavir and lopinavir was also found to be associated with decreased haemolysin activities (p < 0.05). The lower proteinase activities in HIV-infected group could be due to the direct effect of protease inhibitors in HAART while the higher phospholipase and haemolytic activity in the HIV-infected group remains elusive. Conclusion: These finding provide evidence of altered extracellular enzyme activities in C. albicans isolates obtained from HIV patients on HAART, which may affect the infectivity of the Candida isolates.
DescriptionSession - Yeasts and Fungi
Persistent Identifierhttp://hdl.handle.net/10722/129600
ISSN
2013 Impact Factor: 4.144
2013 SCImago Journal Rankings: 1.492

 

DC FieldValueLanguage
dc.contributor.authorTsang, PCSen_US
dc.contributor.authorThomas, Sen_US
dc.contributor.authorPerera, RAPMen_US
dc.date.accessioned2010-12-23T08:39:48Z-
dc.date.available2010-12-23T08:39:48Z-
dc.date.issued2010en_US
dc.identifier.citationThe 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/129600-
dc.descriptionSession - Yeasts and Fungi-
dc.description.abstractThe mortality and morbidity of HIV-infected patients have declined with the advent of highly active antiretroviral therapy (HAART). However, its effect on Candida infections remains inconclusive. Objectives: To determine the phospholipase, proteinase and haemoltyic activities of oral C. albicans in HIV-infected patients on HAART, and the factors affecting their activities. Methods: Altogether 32 C. albicans isolates were tested in vitro for phospholipase, proteinase and haemolytic activities using three different plate assays. Control group comprised of C. albicans collected from 16 healthy individuals. Simple regression was used to study the correlation of the enzymatic activities with gender, age, education level, income, HIV stage of infection, current medications, duration on HAART, history of oral candidiasis, HIV viral load, CD4 count and salivary flow rate. Results: Phospholipase and haemolytic activities were found to be significantly higher (p < 0.05) and proteinase activities were lower in the test group (p < 0.05). There was a significant correlation of the pH value of saliva with the phospholipase activities in the test group (R Sq Linear = 0.594). Patients taking abacavir, a nucleoside reverse transcriptase inhibitor (NRTI), were found to have higher proteinase activities (p < 0.05). However, haemolysin activities were significantly lower in patients taking abacavir (p < 0.05). The use of protease inhibitors ritonavir and lopinavir was also found to be associated with decreased haemolysin activities (p < 0.05). The lower proteinase activities in HIV-infected group could be due to the direct effect of protease inhibitors in HAART while the higher phospholipase and haemolytic activity in the HIV-infected group remains elusive. Conclusion: These finding provide evidence of altered extracellular enzyme activities in C. albicans isolates obtained from HIV patients on HAART, which may affect the infectivity of the Candida isolates.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..-
dc.subjectMedical sciences-
dc.subjectDentistry-
dc.titleFactors affecting Candida albicans exoenzyme activities in HIV-infected patientsen_US
dc.typeConference_Paperen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0345&volume=89 &issue=Spec. Iss B&spage=abstract no. 1795&epage=&date=2010&atitle=Factors+affecting+Candida+albicans+exoenzyme+activities+in+HIV-infected+patients-
dc.identifier.emailTsang, PCS: csptsang@hkucc.hku.hken_US
dc.identifier.hkuros177464en_US
dc.identifier.volume89-
dc.identifier.issueSpec. Iss B-
dc.description.otherThe 88th General Session & Exhibition of the International Association of Dental Research (IADR), Barcelona, Spain, 14-17 July 2010. In Journal of Dental Research, 2010, v. 89, Spec. Iss B, abstract no. 1795-

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