File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An in vitro multi-parametric approach to measuring the effect of implant surface characteristics on cell behaviour

TitleAn in vitro multi-parametric approach to measuring the effect of implant surface characteristics on cell behaviour
Authors
Issue Date2010
PublisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMM
Citation
Biomedical Materials, 2010, v. 5 n. 1, article no. 15002 How to Cite?
AbstractOrthopaedic implants are designed to promote biocompatibility and hence their integration with surrounding tissue. This involves influencing cell-implant interactions through changes in both surface topography and surface roughness. However, the large range of machining techniques used in implant manufacture and inconsistencies in the measurement techniques used for surface characterization make it difficult to measure the impact of surface characteristics on cell-implant interactions. Here, we describe a new in vitro multi-parametric approach that uses commercially available arrays of engineered surfaces that linearly increase in roughness, as measured by Ra, and that can be used to obtain quantitative measurements of cell attachment, differentiation and bone formation. Using this model, we demonstrate that cell attachment above 50% confluency occurs over a narrow range of roughness (Ra from 0.0125 νm to 6.3 νm) and that promotion of cell differentiation and bone development, while significantly influenced by surface topography, does not correlate directly with initial levels of cell attachment. These results compare well with published in vivo implant biocompatibility data indicating that this approach has the potential to offer a rapid, reliable and reproducible in vitro prediction of in vivo implant biocompatibility. © 2010 IOP Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/129337
ISSN
2015 Impact Factor: 3.361
2015 SCImago Journal Rankings: 0.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDavies, JTen_HK
dc.contributor.authorLam, Jen_HK
dc.contributor.authorTomlins, PEen_HK
dc.contributor.authorMarshall, Den_HK
dc.date.accessioned2010-12-23T08:35:31Z-
dc.date.available2010-12-23T08:35:31Z-
dc.date.issued2010en_HK
dc.identifier.citationBiomedical Materials, 2010, v. 5 n. 1, article no. 15002en_HK
dc.identifier.issn1748-6041en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129337-
dc.description.abstractOrthopaedic implants are designed to promote biocompatibility and hence their integration with surrounding tissue. This involves influencing cell-implant interactions through changes in both surface topography and surface roughness. However, the large range of machining techniques used in implant manufacture and inconsistencies in the measurement techniques used for surface characterization make it difficult to measure the impact of surface characteristics on cell-implant interactions. Here, we describe a new in vitro multi-parametric approach that uses commercially available arrays of engineered surfaces that linearly increase in roughness, as measured by Ra, and that can be used to obtain quantitative measurements of cell attachment, differentiation and bone formation. Using this model, we demonstrate that cell attachment above 50% confluency occurs over a narrow range of roughness (Ra from 0.0125 νm to 6.3 νm) and that promotion of cell differentiation and bone development, while significantly influenced by surface topography, does not correlate directly with initial levels of cell attachment. These results compare well with published in vivo implant biocompatibility data indicating that this approach has the potential to offer a rapid, reliable and reproducible in vitro prediction of in vivo implant biocompatibility. © 2010 IOP Publishing Ltd.en_HK
dc.languageengen_US
dc.publisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/BMMen_HK
dc.relation.ispartofBiomedical Materialsen_HK
dc.titleAn in vitro multi-parametric approach to measuring the effect of implant surface characteristics on cell behaviouren_HK
dc.typeArticleen_HK
dc.identifier.emailLam, J: jkwlam@hku.hken_HK
dc.identifier.authorityLam, J=rp01346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1088/1748-6041/5/1/015002en_HK
dc.identifier.pmid20057015-
dc.identifier.scopuseid_2-s2.0-76249093645en_HK
dc.identifier.hkuros176479en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-76249093645&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue1en_HK
dc.identifier.spage15002en_US
dc.identifier.spagearticle no. 15002-
dc.identifier.epagearticle no. 15002-
dc.identifier.isiWOS:000274247400002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDavies, JT=35721763000en_HK
dc.identifier.scopusauthoridLam, J=8404243000en_HK
dc.identifier.scopusauthoridTomlins, PE=6701753012en_HK
dc.identifier.scopusauthoridMarshall, D=23013051700en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats