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Article: Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions

TitlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
Authors
KeywordsElectrospinning
Neural tissue engineering
Photochemical crosslinking
Type I collagen
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/
Citation
Journal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282 How to Cite?
Abstract
Currently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/129261
ISSN
2013 Impact Factor: 2.841
2013 SCImago Journal Rankings: 1.079
ISI Accession Number ID
Funding AgencyGrant Number
National Medical Research Council (NMRC)NMRC/EDG/0027/2008
A*Star BMRC07/1/22/19/519
AOSpine Award, Hong KongAOSBR-07-06
Funding Information:

Contract grant sponsor: National Medical Research Council (NMRC) Exploratory/Development Grant; contract grant number: NMRC/EDG/0027/2008

References
Grants

 

Author Affiliations
  1. The University of Hong Kong
  2. Nanyang Technological University
DC FieldValueLanguage
dc.contributor.authorLiu, Ten_HK
dc.contributor.authorTeng, WKen_HK
dc.contributor.authorChan, BPen_HK
dc.contributor.authorChew, SYen_HK
dc.date.accessioned2010-12-23T08:34:22Z-
dc.date.available2010-12-23T08:34:22Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282en_HK
dc.identifier.issn1549-3296en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129261-
dc.description.abstractCurrently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/en_HK
dc.relation.ispartofJournal of Biomedical Materials Research - Part Aen_HK
dc.rightsJournal of Biomedical Materials Research Part A. Copyright © John Wiley & Sons, Inc.-
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.subjectElectrospinningen_HK
dc.subjectNeural tissue engineeringen_HK
dc.subjectPhotochemical crosslinkingen_HK
dc.subjectType I collagenen_HK
dc.titlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1549-3296&volume=95&issue=1&spage=276&epage=282&date=2010&atitle=Photochemical+crosslinked+electrospun+collagen+nanofibers:+synthesis,+characterization+and+neural+stem+cell+interactions-
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.a.32831en_HK
dc.identifier.pmid20607867-
dc.identifier.scopuseid_2-s2.0-77956472536en_HK
dc.identifier.hkuros177257en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956472536&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue1en_HK
dc.identifier.spage276en_HK
dc.identifier.epage282en_HK
dc.identifier.isiWOS:000281448700028-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectBiomaterials-assisted cell-based therapy for disc degeneration-
dc.identifier.scopusauthoridLiu, T=9736097100en_HK
dc.identifier.scopusauthoridTeng, WK=36515576400en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.scopusauthoridChew, SY=8656381200en_HK

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