Article: Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions

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TitlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
AuthorsLiu, T2
Teng, WK1
Chan, BP1
Chew, SY2
KeywordsElectrospinning
Neural tissue engineering
Photochemical crosslinking
Type I collagen
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/
CitationJournal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jbm.a.32831
AbstractCurrently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.
ISSN1549-3296
2011 Impact Factor: 2.625
2011 SCImago Journal Rankings: 0.198
DOIhttp://dx.doi.org/10.1002/jbm.a.32831
ReferencesReferences in Scopus
GrantsBiomaterials-assisted cell-based therapy for disc degeneration
DC Field
Value
dc.contributor.authorLiu, T
dc.contributor.authorTeng, WK
dc.contributor.authorChan, BP
dc.contributor.authorChew, SY
dc.date.accessioned2010-12-23T08:34:22Z
dc.date.available2010-12-23T08:34:22Z
dc.date.issued2010
dc.description.abstractCurrently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.
dc.description.grantBiomaterials-assisted cell-based therapy for disc degeneration
dc.description.grantcode97224
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jbm.a.32831
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.a.32831
dc.identifier.epage282
dc.identifier.hkuros177257
dc.identifier.isiWOS:000281448700028
Funding AgencyGrant Number
National Medical Research Council (NMRC)NMRC/EDG/0027/2008
A*Star BMRC07/1/22/19/519
AOSpine Award, Hong KongAOSBR-07-06
Funding Information:

Contract grant sponsor: National Medical Research Council (NMRC) Exploratory/Development Grant; contract grant number: NMRC/EDG/0027/2008

dc.identifier.issn1549-3296
2011 Impact Factor: 2.625
2011 SCImago Journal Rankings: 0.198
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid20607867
dc.identifier.scopuseid_2-s2.0-77956472536
dc.identifier.spage276
dc.identifier.urihttp://hdl.handle.net/10722/129261
dc.identifier.volume95
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Biomedical Materials Research - Part A
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Biomedical Materials Research Part A. Copyright © John Wiley & Sons, Inc.
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)
dc.subjectElectrospinning
dc.subjectNeural tissue engineering
dc.subjectPhotochemical crosslinking
dc.subjectType I collagen
dc.titlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Nanyang Technological University