File Download
  • No File Attached
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
  • Basic View
  • Metadata View
  • XML View
TitlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
 
AuthorsLiu, T2
Teng, WK1
Chan, BP1
Chew, SY2
 
KeywordsElectrospinning
Neural tissue engineering
Photochemical crosslinking
Type I collagen
 
Issue Date2010
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/
 
CitationJournal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jbm.a.32831
 
AbstractCurrently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.
 
ISSN1549-3296
2012 Impact Factor: 2.834
2012 SCImago Journal Rankings: 1.016
 
DOIhttp://dx.doi.org/10.1002/jbm.a.32831
 
ISI Accession Number IDWOS:000281448700028
Funding AgencyGrant Number
National Medical Research Council (NMRC)NMRC/EDG/0027/2008
A*Star BMRC07/1/22/19/519
AOSpine Award, Hong KongAOSBR-07-06
Funding Information:

Contract grant sponsor: National Medical Research Council (NMRC) Exploratory/Development Grant; contract grant number: NMRC/EDG/0027/2008

 
ReferencesReferences in Scopus
 
GrantsBiomaterials-assisted cell-based therapy for disc degeneration
 
DC FieldValue
dc.contributor.authorLiu, T
 
dc.contributor.authorTeng, WK
 
dc.contributor.authorChan, BP
 
dc.contributor.authorChew, SY
 
dc.date.accessioned2010-12-23T08:34:22Z
 
dc.date.available2010-12-23T08:34:22Z
 
dc.date.issued2010
 
dc.description.abstractCurrently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 ± 5.9% vs. 12.5 ± 2.0%, p < 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37°C, indicated a mass loss of 47.7 ± 7.4% and 68.9 ± 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. © 2010 Wiley Periodicals, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jbm.a.32831
 
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.a.32831
 
dc.identifier.epage282
 
dc.identifier.hkuros177257
 
dc.identifier.isiWOS:000281448700028
Funding AgencyGrant Number
National Medical Research Council (NMRC)NMRC/EDG/0027/2008
A*Star BMRC07/1/22/19/519
AOSpine Award, Hong KongAOSBR-07-06
Funding Information:

Contract grant sponsor: National Medical Research Council (NMRC) Exploratory/Development Grant; contract grant number: NMRC/EDG/0027/2008

 
dc.identifier.issn1549-3296
2012 Impact Factor: 2.834
2012 SCImago Journal Rankings: 1.016
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid20607867
 
dc.identifier.scopuseid_2-s2.0-77956472536
 
dc.identifier.spage276
 
dc.identifier.urihttp://hdl.handle.net/10722/129261
 
dc.identifier.volume95
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biomedical Materials Research - Part A
 
dc.relation.projectBiomaterials-assisted cell-based therapy for disc degeneration
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Biomedical Materials Research Part A. Copyright © John Wiley & Sons, Inc.
 
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)
 
dc.subjectElectrospinning
 
dc.subjectNeural tissue engineering
 
dc.subjectPhotochemical crosslinking
 
dc.subjectType I collagen
 
dc.titlePhotochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Liu, T</contributor.author>
<contributor.author>Teng, WK</contributor.author>
<contributor.author>Chan, BP</contributor.author>
<contributor.author>Chew, SY</contributor.author>
<date.accessioned>2010-12-23T08:34:22Z</date.accessioned>
<date.available>2010-12-23T08:34:22Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>Journal Of Biomedical Materials Research - Part A, 2010, v. 95 n. 1, p. 276-282</identifier.citation>
<identifier.issn>1549-3296</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/129261</identifier.uri>
<description.abstract>Currently available crosslinking methods for electrospun collagen nanofibers do not preserve the fibrous architecture over prolonged periods of time. In addition, electrospinning of collagen often involves solvents that lead to extensive protein denaturation. In this study, we demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorismanalyses, we demonstrate the fraction of collagen helical structure to be significantly greater in acetic acid-spun fibers than HFP-spun fibers (28.9 &#177; 5.9% vs. 12.5 &#177; 2.0%, p &lt; 0.05). By introducing 0.1% (w/v) rose bengal into collagen fibers and subjecting these scaffolds to laser irradiation at a wavelength of 514 nm for 100 sec, biodegradable crosslinked scaffolds were obtained. Scaffold degradation as evaluated by soaking crosslinked collagen scaffolds in PBS at 37&#176;C, indicated a mass loss of 47.7 &#177; 7.4% and 68.9 &#177; 24.7% at day 7 and day 15, respectively. However, these scaffolds retained fibrous architecture for at least 21 days under physiological conditions. Neural stem cell line, C17.2, cultured on crosslinked collagen scaffolds proliferated after 7 days by forming a confluent layer of cells with extensive cellular projections that were indicative of neurite outgrowth. Taken together, these findings support the potential of acetic acid-electrospun photochemical crosslinked collagen nanofibers for neural tissue engineering. &#169; 2010 Wiley Periodicals, Inc.</description.abstract>
<language>eng</language>
<publisher>John Wiley &amp; Sons, Inc. The Journal&apos;s web site is located at http://www.interscience.wiley.com/jpages/0021-9304/</publisher>
<relation.ispartof>Journal of Biomedical Materials Research - Part A</relation.ispartof>
<rights>Journal of Biomedical Materials Research Part A. Copyright &#169; John Wiley &amp; Sons, Inc.</rights>
<rights>Special Statement for Preprint only

Before publication:
&apos;This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright &#169; ([year]) ([Pathological Society of Great Britain and Ireland])&apos;. 

After publication: the preprint notice should be amended to follows: 
&apos;This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]&apos;

For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement &amp; acknowledgement :

&#8216;This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.&#8217; Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX)

(This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)</rights>
<subject>Electrospinning</subject>
<subject>Neural tissue engineering</subject>
<subject>Photochemical crosslinking</subject>
<subject>Type I collagen</subject>
<title>Photochemical crosslinked electrospun collagen nanofibers: Synthesis, characterization and neural stem cell interactions</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=1549-3296&amp;volume=95&amp;issue=1&amp;spage=276&amp;epage=282&amp;date=2010&amp;atitle=Photochemical+crosslinked+electrospun+collagen+nanofibers:+synthesis,+characterization+and+neural+stem+cell+interactions</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1002/jbm.a.32831</identifier.doi>
<identifier.pmid>20607867</identifier.pmid>
<identifier.scopus>eid_2-s2.0-77956472536</identifier.scopus>
<identifier.hkuros>177257</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-77956472536&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>95</identifier.volume>
<identifier.issue>1</identifier.issue>
<identifier.spage>276</identifier.spage>
<identifier.epage>282</identifier.epage>
<identifier.isi>WOS:000281448700028</identifier.isi>
<publisher.place>United States</publisher.place>
<relation.project>Biomaterials-assisted cell-based therapy for disc degeneration</relation.project>
</item>
Author Affiliations
  1. The University of Hong Kong
  2. Nanyang Technological University